MTP12_ARATH
ID MTP12_ARATH Reviewed; 300 AA.
AC Q9SI03; F4IFA1;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Metal tolerance protein 12;
DE Short=AtMTP12;
GN Name=MTP12; OrderedLocusNames=At2g04620; ORFNames=F7D11.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: Involved in sequestration of excess metal in the cytoplasm
CC into vacuoles to maintain metal homeostasis. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Note=Tonoplast.
CC -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC transporter (TC 2.A.4) family. SLC30A subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AEC05849.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007231; AAD32753.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05849.1; ALT_SEQ; Genomic_DNA.
DR PIR; D84459; D84459.
DR RefSeq; NP_178539.2; NM_126491.3.
DR AlphaFoldDB; Q9SI03; -.
DR SMR; Q9SI03; -.
DR BioGRID; 405; 1.
DR IntAct; Q9SI03; 2.
DR TCDB; 2.A.4.4.9; the cation diffusion facilitator (cdf) family.
DR PaxDb; Q9SI03; -.
DR PeptideAtlas; Q9SI03; -.
DR PRIDE; Q9SI03; -.
DR EnsemblPlants; AT2G04620.2; AT2G04620.2; AT2G04620.
DR GeneID; 815004; -.
DR Gramene; AT2G04620.2; AT2G04620.2; AT2G04620.
DR KEGG; ath:AT2G04620; -.
DR Araport; AT2G04620; -.
DR TAIR; locus:2052311; AT2G04620.
DR eggNOG; KOG1484; Eukaryota.
DR InParanoid; Q9SI03; -.
DR OrthoDB; 1507860at2759; -.
DR PRO; PR:Q9SI03; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SI03; baseline and differential.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; HDA:TAIR.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046873; F:metal ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006882; P:cellular zinc ion homeostasis; IEA:InterPro.
DR GO; GO:0030001; P:metal ion transport; IBA:GO_Central.
DR GO; GO:0071578; P:zinc ion import across plasma membrane; IGI:TAIR.
DR Gene3D; 1.20.1510.10; -; 1.
DR InterPro; IPR002524; Cation_efflux.
DR InterPro; IPR027469; Cation_efflux_TMD_sf.
DR InterPro; IPR045316; Msc2-like.
DR PANTHER; PTHR45755; PTHR45755; 1.
DR Pfam; PF01545; Cation_efflux; 1.
DR SUPFAM; SSF161111; SSF161111; 1.
DR TIGRFAMs; TIGR01297; CDF; 1.
PE 3: Inferred from homology;
KW Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..300
FT /note="Metal tolerance protein 12"
FT /id="PRO_0000400008"
FT TOPO_DOM 1..26
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 48..50
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 51..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 72..91
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 113..128
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..160
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 182..186
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 208..300
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 300 AA; 33071 MW; 6A184AF3DD1B0914 CRC64;
MESPESFSTM FMKPIRHILS EKKSRKIALF LLINTAYMVV EFVAGFMSNS LGLISDACHM
LFDCAALAIG LYASYISRLP ANHQYNYGRG RFEVLSGYVN AVFLVLVGAL IVLESIERIL
DPQEISTNSL LVVSVGGLLV NIVGLIFFHE EHHHAHGGSG IFLHVLADTM GSVGVVISTL
LIKYKGWLVA DPASSIFISI LIIASVIPLL RNSAEILLQR VPRAHRQDLK EAMRNILKTK
GVCSIQRLHV WSFTNSDVVA TLHLLVSADS DKTDTKLQVS RLLEDAGVKD WTLQVESVNS
