MTP8_ORYSJ
ID MTP8_ORYSJ Reviewed; 316 AA.
AC Q8H329; Q7XZF0;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Metal tolerance protein 8;
DE Short=OsMTP8;
GN Name=MTP8; OrderedLocusNames=Os08g0422200, LOC_Os08g32650;
GN ORFNames=OSJNBa0077M12.119;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Jiu Caiqing; TISSUE=Root;
RA Huang J., Jiang Y., Zhang H.-S.;
RT "Molecular cloning and characterization of a cDNA encoding cation
RT transporter from rice.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Involved in sequestration of excess metal in the cytoplasm
CC into vacuoles to maintain metal homeostasis. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Note=Tonoplast.
CC -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC transporter (TC 2.A.4) family. SLC30A subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAP74355.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
CC Sequence=BAF23740.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY305863; AAP74355.1; ALT_SEQ; mRNA.
DR EMBL; AP005301; BAC24961.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF23740.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014964; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015650558.1; XM_015795072.1.
DR AlphaFoldDB; Q8H329; -.
DR SMR; Q8H329; -.
DR STRING; 4530.OS08T0422200-00; -.
DR PaxDb; Q8H329; -.
DR PRIDE; Q8H329; -.
DR GeneID; 4345598; -.
DR KEGG; osa:4345598; -.
DR eggNOG; KOG1484; Eukaryota.
DR InParanoid; Q8H329; -.
DR OrthoDB; 973492at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046873; F:metal ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006882; P:cellular zinc ion homeostasis; IEA:InterPro.
DR GO; GO:0030001; P:metal ion transport; IBA:GO_Central.
DR Gene3D; 1.20.1510.10; -; 1.
DR InterPro; IPR002524; Cation_efflux.
DR InterPro; IPR036837; Cation_efflux_CTD_sf.
DR InterPro; IPR027469; Cation_efflux_TMD_sf.
DR InterPro; IPR045316; Msc2-like.
DR PANTHER; PTHR45755; PTHR45755; 1.
DR Pfam; PF01545; Cation_efflux; 1.
DR SUPFAM; SSF160240; SSF160240; 1.
DR SUPFAM; SSF161111; SSF161111; 1.
DR TIGRFAMs; TIGR01297; CDF; 1.
PE 2: Evidence at transcript level;
KW Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..316
FT /note="Metal tolerance protein 8"
FT /id="PRO_0000400016"
FT TOPO_DOM 1..15
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..45
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..80
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..116
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 138..176
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 198..202
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 224..316
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 147..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 316 AA; 35039 MW; D40BA8D28C9DDD13 CRC64;
MGPVRHILNE RKSRKIAAFL LINTAYMFVE FTSGFMSDSL GLISDACHML FDCAALAIGL
YASYIARLPA NGLYNYGRGR FEVLSGYVNA VFLVLVGALI VLESFERILE PREISTSSLL
TVSIGGLVVN VIGLVFFHEE HHHAHGEAHS CNGGLQSSEN HNKSRNRHHI DHNMEGIFLH
VLADTMGSVG VVISTLLIKY KGWLIADPIC SVFISIMIVS SVLPLLRNSA EILLQRVPRS
LEKDIKEALD DVMKIKGVIG VHNFHVWNLT NTDIVGTFHL HITTEADKSS IREKASDIFH
EAGIQDLTIQ IECVKR
