ID   MTP9_ARATH              Reviewed;         402 AA.
AC   Q9SAJ7;
DT   02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 2.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Metal tolerance protein 9;
DE            Short=AtMTP9;
GN   Name=MTP9; OrderedLocusNames=At1g79520; ORFNames=T8K14.6;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
CC   -!- FUNCTION: Involved in sequestration of excess metal in the cytoplasm
CC       into vacuoles to maintain metal homeostasis. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}. Note=Tonoplast.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q9SAJ7-1; Sequence=Displayed;
CC   -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC       transporter (TC 2.A.4) family. SLC30A subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD30224.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC007202; AAD30224.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE36258.1; -; Genomic_DNA.
DR   PIR; E96826; E96826.
DR   RefSeq; NP_178070.2; NM_106600.3. [Q9SAJ7-1]
DR   AlphaFoldDB; Q9SAJ7; -.
DR   STRING; 3702.AT1G79520.2; -.
DR   iPTMnet; Q9SAJ7; -.
DR   PaxDb; Q9SAJ7; -.
DR   PRIDE; Q9SAJ7; -.
DR   EnsemblPlants; AT1G79520.1; AT1G79520.1; AT1G79520. [Q9SAJ7-1]
DR   GeneID; 844290; -.
DR   Gramene; AT1G79520.1; AT1G79520.1; AT1G79520. [Q9SAJ7-1]
DR   KEGG; ath:AT1G79520; -.
DR   Araport; AT1G79520; -.
DR   eggNOG; KOG1485; Eukaryota.
DR   HOGENOM; CLU_013430_2_3_1; -.
DR   InParanoid; Q9SAJ7; -.
DR   PhylomeDB; Q9SAJ7; -.
DR   PRO; PR:Q9SAJ7; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9SAJ7; baseline and differential.
DR   Genevisible; Q9SAJ7; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008324; F:cation transmembrane transporter activity; IBA:GO_Central.
DR   Gene3D; 1.20.1510.10; -; 1.
DR   Gene3D; 3.30.70.1350; -; 1.
DR   InterPro; IPR002524; Cation_efflux.
DR   InterPro; IPR027470; Cation_efflux_CTD.
DR   InterPro; IPR036837; Cation_efflux_CTD_sf.
DR   InterPro; IPR027469; Cation_efflux_TMD_sf.
DR   Pfam; PF01545; Cation_efflux; 1.
DR   Pfam; PF16916; ZT_dimer; 1.
DR   SUPFAM; SSF160240; SSF160240; 1.
DR   SUPFAM; SSF161111; SSF161111; 1.
DR   TIGRFAMs; TIGR01297; CDF; 1.
PE   3: Inferred from homology;
KW   Alternative splicing; Ion transport; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport; Vacuole.
FT   CHAIN           1..402
FT                   /note="Metal tolerance protein 9"
FT                   /id="PRO_0000400005"
FT   TOPO_DOM        1..114
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        115..135
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        136..140
FT                   /note="Vacuolar"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        141..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        162..182
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        183..203
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        204..221
FT                   /note="Vacuolar"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        222..242
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        243..257
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        258..278
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        279..284
FT                   /note="Vacuolar"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        285..305
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        306..402
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   402 AA;  45561 MW;  0107F765007B3D38 CRC64;
     MAATEHRGLS GGDYNVDLLP IDQDDSPPSS WRLSLDTFRL PSSSPLSSGR HNGRTRLSRY
     LRTPKKERKV SEYYKQQEKL LEGFNEMETI NETGFVSGAP TEEELKKLAK SERLAVHISN
     AANLVLFVAK VYASVESRSM AVIASTLDSL LDLLSGFILW FTANAMRTPN NFRYPIGKRR
     MQPVGIIVFA SVMATLGLQV ILESTRLLVS KNGSHMSSTE EKWMIGIMAS ATVVKFLLML
     YCRSFQNEIV RAYAQDHLFD VITNSVGLAT AVLAVKFYWW IDPSGAILIA LYTISTWART
     VLENVHSLIG RSAPPDFLAK LTFLIWNHHE KIKHIDTVRA YTFGSHYFVE VDIVLPEDMR
     LHEAHNIGET LQEKLEQLSE VERAFVHIDF EFTHRPEHKC KV