MTPB_ARATH
ID MTPB_ARATH Reviewed; 375 AA.
AC Q6DBM8; Q9ZW23;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Metal tolerance protein B;
DE Short=AtMTP4;
DE Short=AtMTPb;
GN Name=MTPB; Synonyms=MTP4; OrderedLocusNames=At2g29410; ORFNames=F16P2.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
CC -!- FUNCTION: Involved in sequestration of excess zinc in the cytoplasm
CC into vacuoles to maintain zinc homeostasis. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Note=Tonoplast.
CC -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC transporter (TC 2.A.4) family. SLC30A subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC95197.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AC004561; AAC95197.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002685; AEC08251.1; -; Genomic_DNA.
DR EMBL; BT014994; AAT70445.1; -; mRNA.
DR EMBL; BT015899; AAU95435.1; -; mRNA.
DR PIR; A84696; A84696.
DR RefSeq; NP_180502.2; NM_128495.4.
DR AlphaFoldDB; Q6DBM8; -.
DR SMR; Q6DBM8; -.
DR BioGRID; 2840; 22.
DR IntAct; Q6DBM8; 22.
DR STRING; 3702.AT2G29410.1; -.
DR PaxDb; Q6DBM8; -.
DR PRIDE; Q6DBM8; -.
DR ProteomicsDB; 251408; -.
DR EnsemblPlants; AT2G29410.1; AT2G29410.1; AT2G29410.
DR GeneID; 817490; -.
DR Gramene; AT2G29410.1; AT2G29410.1; AT2G29410.
DR KEGG; ath:AT2G29410; -.
DR Araport; AT2G29410; -.
DR TAIR; locus:2043117; AT2G29410.
DR eggNOG; KOG1482; Eukaryota.
DR HOGENOM; CLU_013430_0_1_1; -.
DR InParanoid; Q6DBM8; -.
DR OMA; RTWGWAR; -.
DR OrthoDB; 973492at2759; -.
DR PhylomeDB; Q6DBM8; -.
DR PRO; PR:Q6DBM8; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q6DBM8; baseline and differential.
DR Genevisible; Q6DBM8; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0071577; P:zinc ion transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1510.10; -; 1.
DR InterPro; IPR002524; Cation_efflux.
DR InterPro; IPR036837; Cation_efflux_CTD_sf.
DR InterPro; IPR027469; Cation_efflux_TMD_sf.
DR Pfam; PF01545; Cation_efflux; 1.
DR SUPFAM; SSF160240; SSF160240; 1.
DR SUPFAM; SSF161111; SSF161111; 1.
DR TIGRFAMs; TIGR01297; CDF; 1.
PE 2: Evidence at transcript level;
KW Ion transport; Membrane; Metal-binding; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Vacuole; Zinc; Zinc transport.
FT CHAIN 1..375
FT /note="Metal tolerance protein B"
FT /id="PRO_0000206119"
FT TOPO_DOM 1..57
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 79..84
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..122
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 144..160
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 182..240
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 262..264
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 286..375
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 182..206
FT /note="Required for zinc-binding"
FT /evidence="ECO:0000250"
SQ SEQUENCE 375 AA; 42340 MW; 0921D759E49DBD80 CRC64;
MELEQICILK PDDEEEMESP SPSKTEENLG VVPLSCAFTR QEHCVSETKE REESTRRLSS
LIFLYLIVMS VQIVGGFKAN SLAVMTDAAH LLSDVAGLCV SLLAIKVSSW EANPRNSFGF
KRLEVLAAFL SVQLIWLVSG VIIHEAIQRL LSRSREVNGE IMFGISAFGF FMNLVMVLWL
GHNHSHHHHD HHHHHHNHKH QHQHHHKEVV AEEEEEEMNP LKGEKSSSKE MNINIQGAYL
HAMADMIQSL GVMIGGGIIW VKPKWVLVDL ICTLVFSAFA LAATLPILKN IFGILMERVP
RDMDIEKLER GLKRIDGVKI VYDLHVWEIT VGRIVLSCHI LPEPGASPKE IITGVRNFCR
KSYGIYHATV QVESE
