MTPC1_ARATH
ID MTPC1_ARATH Reviewed; 471 AA.
AC Q8L725; O82250;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Metal tolerance protein C1;
DE Short=AtMTPc1;
DE AltName: Full=AtMTP6;
GN Name=MTPC1; Synonyms=MTP6; OrderedLocusNames=At2g47830; ORFNames=F17A22.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
CC -!- FUNCTION: Involved in sequestration of excess metal in the cytoplasm
CC into vacuoles to maintain metal homeostasis. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Note=Tonoplast.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8L725-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8L725-2; Sequence=VSP_015095;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing donor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC transporter (TC 2.A.4) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC63637.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC005309; AAC63637.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10893.1; -; Genomic_DNA.
DR EMBL; AY139998; AAM98140.1; -; mRNA.
DR EMBL; BT006611; AAP31955.1; -; mRNA.
DR PIR; A84920; A84920.
DR RefSeq; NP_182304.2; NM_130350.3. [Q8L725-1]
DR AlphaFoldDB; Q8L725; -.
DR STRING; 3702.AT2G47830.1; -.
DR TCDB; 2.A.4.7.2; the cation diffusion facilitator (cdf) family.
DR iPTMnet; Q8L725; -.
DR PaxDb; Q8L725; -.
DR PRIDE; Q8L725; -.
DR EnsemblPlants; AT2G47830.1; AT2G47830.1; AT2G47830. [Q8L725-1]
DR GeneID; 819395; -.
DR Gramene; AT2G47830.1; AT2G47830.1; AT2G47830. [Q8L725-1]
DR KEGG; ath:AT2G47830; -.
DR Araport; AT2G47830; -.
DR TAIR; locus:2043328; AT2G47830.
DR eggNOG; KOG1485; Eukaryota.
DR InParanoid; Q8L725; -.
DR OMA; PNINAIW; -.
DR OrthoDB; 667718at2759; -.
DR PhylomeDB; Q8L725; -.
DR PRO; PR:Q8L725; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8L725; baseline and differential.
DR Genevisible; Q8L725; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IBA:GO_Central.
DR Gene3D; 1.20.1510.10; -; 1.
DR Gene3D; 3.30.70.1350; -; 1.
DR InterPro; IPR002524; Cation_efflux.
DR InterPro; IPR027470; Cation_efflux_CTD.
DR InterPro; IPR036837; Cation_efflux_CTD_sf.
DR InterPro; IPR027469; Cation_efflux_TMD_sf.
DR Pfam; PF01545; Cation_efflux; 1.
DR Pfam; PF16916; ZT_dimer; 1.
DR SUPFAM; SSF160240; SSF160240; 1.
DR SUPFAM; SSF161111; SSF161111; 1.
DR TIGRFAMs; TIGR01297; CDF; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..471
FT /note="Metal tolerance protein C1"
FT /id="PRO_0000206120"
FT TOPO_DOM 1..78
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 100..101
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 123..144
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 166..192
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 214..236
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 258..423
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 424..444
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 445..471
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VAR_SEQ 437..439
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_015095"
SQ SEQUENCE 471 AA; 51027 MW; 9E305E0F3B994B11 CRC64;
MGIIRFQILN PTRICRSTIY YMSCRYLPSS SSSSSPLRVS SSSSQKPSFD FSRRWHFGHP
DHHQQYQKPG EEGEKIFRLG LTADIGLSVA KALTGYLCGS TAIIADAAHS VSDVVLSGVA
LVSYRAANVP KDKEHPYGHG KFETLGALGI SAMLLATGSG IAWHALDLLS IALSAAPEVI
HSGHHHGIDM NHPILALTVT IASISIKEGL YWITKRAGEK QGSGLMMANA WHHRSDAISS
LVALVGVGGS ILGVNFLDPL AGLVVSTMIV NAGLKTGHQS ILELVDAAIP AQQLEPIRQT
ILQVEGVKGC HRLRGRRAGS SLYLDVHIVV DPFSSVSVAH EVGEYVRRQI NLNHPEVSEV
FIHIDPAFLQ FSCSTKDHDS ITKESNICQE IKHVEATVSD IFSSQLSEKL TIKRITPHLL
HSKILLQIVV AMPSTMSIQD VMIAAEHAEK EILKAAPNVA RVSIQLSLNS E
