MTPC2_ARATH
ID MTPC2_ARATH Reviewed; 393 AA.
AC Q6ICY4; Q9C7C2; Q9LH56;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Metal tolerance protein C2;
DE Short=AtMTPc2;
DE AltName: Full=AtMTP5;
GN Name=MTPC2; Synonyms=MTP5; OrderedLocusNames=At3g12100; ORFNames=T21B14.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
CC -!- FUNCTION: Involved in sequestration of excess metal in the cytoplasm
CC into vacuoles to maintain metal homeostasis. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Note=Tonoplast.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q6ICY4-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC transporter (TC 2.A.4) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51048.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAB01958.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP002063; BAB01958.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC069473; AAG51048.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE75149.1; -; Genomic_DNA.
DR EMBL; BT014889; AAT44130.1; -; mRNA.
DR EMBL; BT015749; AAU84686.1; -; mRNA.
DR RefSeq; NP_187817.2; NM_112045.5. [Q6ICY4-1]
DR AlphaFoldDB; Q6ICY4; -.
DR BioGRID; 5718; 1.
DR STRING; 3702.AT3G12100.1; -.
DR TCDB; 2.A.4.4.6; the cation diffusion facilitator (cdf) family.
DR iPTMnet; Q6ICY4; -.
DR PaxDb; Q6ICY4; -.
DR PRIDE; Q6ICY4; -.
DR ProteomicsDB; 251307; -. [Q6ICY4-1]
DR EnsemblPlants; AT3G12100.1; AT3G12100.1; AT3G12100. [Q6ICY4-1]
DR GeneID; 820384; -.
DR Gramene; AT3G12100.1; AT3G12100.1; AT3G12100. [Q6ICY4-1]
DR KEGG; ath:AT3G12100; -.
DR Araport; AT3G12100; -.
DR TAIR; locus:2099307; AT3G12100.
DR eggNOG; KOG1484; Eukaryota.
DR InParanoid; Q6ICY4; -.
DR OMA; SKCWRQI; -.
DR PhylomeDB; Q6ICY4; -.
DR PRO; PR:Q6ICY4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q6ICY4; baseline and differential.
DR Genevisible; Q6ICY4; AT.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046873; F:metal ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006882; P:cellular zinc ion homeostasis; IEA:InterPro.
DR GO; GO:0030001; P:metal ion transport; IBA:GO_Central.
DR GO; GO:0009624; P:response to nematode; HEP:TAIR.
DR Gene3D; 1.20.1510.10; -; 1.
DR InterPro; IPR002524; Cation_efflux.
DR InterPro; IPR027469; Cation_efflux_TMD_sf.
DR InterPro; IPR045316; Msc2-like.
DR PANTHER; PTHR45755; PTHR45755; 1.
DR Pfam; PF01545; Cation_efflux; 1.
DR SUPFAM; SSF161111; SSF161111; 1.
DR TIGRFAMs; TIGR01297; CDF; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..393
FT /note="Metal tolerance protein C2"
FT /id="PRO_0000206121"
FT TOPO_DOM 1..113
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 135..139
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 161..186
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 208..214
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..259
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 281..283
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..304
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 305..393
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 393 AA; 43815 MW; CCEC26838137DAEF CRC64;
MERSISFNPR GDNELPDDRS SDVGYAANDR RLAYSRSFQQ SHGPRTPAVT EAAKPFLDRT
VSSIDMPPDI YSVDGSDVFF GEGKDVDMAK VSVLEMVWEV FGVVTSGNRQ MKRLFLLIAL
NVLYSTTELS IGIFTGRVGL VSDAFHLTFG CGLLTFSLFA MATSRKKPDH AYSYGYKRLE
VLSAFTNALF LMFMSFSLAV EALHAFIQDE SEHKHYLIVS AVTNLLVNLL GVWFFRNYAR
VNIAYRKAED MNYHSVCLHV ISDSIRSAGL ILASWLLSLG VENAEVLCLG LVSVTVFMLV
MPLFKATGGV LLQMAPPNIP SSALSKCLRQ ITSREDVTEV LQARFWEVVP GHTVGSLRLQ
VKSGIDERPL LQYVYDVYHD LGVQDLTLQT DYT
