MTPC3_ARATH
ID MTPC3_ARATH Reviewed; 411 AA.
AC Q9M2P2;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Putative metal tolerance protein C3;
DE Short=AtMTPc3;
DE AltName: Full=AtMTP8;
GN Name=MTPC3; Synonyms=MTP8; OrderedLocusNames=At3g58060;
GN ORFNames=T10K17.270;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
CC -!- FUNCTION: Involved in sequestration of excess metal in the cytoplasm
CC into vacuoles to maintain metal homeostasis. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Note=Tonoplast.
CC -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC transporter (TC 2.A.4) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB67634.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL132977; CAB67634.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE79737.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64639.1; -; Genomic_DNA.
DR PIR; T46028; T46028.
DR RefSeq; NP_001319789.1; NM_001339906.1.
DR RefSeq; NP_191365.2; NM_115668.3.
DR AlphaFoldDB; Q9M2P2; -.
DR STRING; 3702.AT3G58060.1; -.
DR TCDB; 2.A.4.5.4; the cation diffusion facilitator (cdf) family.
DR PaxDb; Q9M2P2; -.
DR PRIDE; Q9M2P2; -.
DR ProteomicsDB; 250990; -.
DR EnsemblPlants; AT3G58060.1; AT3G58060.1; AT3G58060.
DR EnsemblPlants; AT3G58060.2; AT3G58060.2; AT3G58060.
DR GeneID; 824975; -.
DR Gramene; AT3G58060.1; AT3G58060.1; AT3G58060.
DR Gramene; AT3G58060.2; AT3G58060.2; AT3G58060.
DR KEGG; ath:AT3G58060; -.
DR Araport; AT3G58060; -.
DR TAIR; locus:2095873; AT3G58060.
DR eggNOG; KOG1485; Eukaryota.
DR HOGENOM; CLU_013430_2_3_1; -.
DR InParanoid; Q9M2P2; -.
DR OMA; VWWLDPV; -.
DR OrthoDB; 874665at2759; -.
DR PhylomeDB; Q9M2P2; -.
DR PRO; PR:Q9M2P2; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M2P2; baseline and differential.
DR Genevisible; Q9M2P2; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:TAIR.
DR GO; GO:0035618; C:root hair; IDA:TAIR.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IGI:TAIR.
DR GO; GO:0006826; P:iron ion transport; IGI:TAIR.
DR GO; GO:0071421; P:manganese ion transmembrane transport; IGI:TAIR.
DR GO; GO:0048316; P:seed development; IEP:TAIR.
DR GO; GO:0009845; P:seed germination; IMP:TAIR.
DR GO; GO:0097577; P:sequestering of iron ion; IGI:TAIR.
DR Gene3D; 1.20.1510.10; -; 1.
DR Gene3D; 3.30.70.1350; -; 1.
DR InterPro; IPR002524; Cation_efflux.
DR InterPro; IPR027470; Cation_efflux_CTD.
DR InterPro; IPR036837; Cation_efflux_CTD_sf.
DR InterPro; IPR027469; Cation_efflux_TMD_sf.
DR Pfam; PF01545; Cation_efflux; 1.
DR Pfam; PF16916; ZT_dimer; 1.
DR SUPFAM; SSF160240; SSF160240; 1.
DR SUPFAM; SSF161111; SSF161111; 1.
DR TIGRFAMs; TIGR01297; CDF; 1.
PE 3: Inferred from homology;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport; Vacuole.
FT CHAIN 1..411
FT /note="Putative metal tolerance protein C3"
FT /id="PRO_0000206122"
FT TOPO_DOM 1..115
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 137..140
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 162..184
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 206..222
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 244..262
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 263..283
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 284..287
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 309..390
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 411 AA; 46241 MW; 05F6A9EA34BFC859 CRC64;
MEVNYCPETP LLSSNDHEAI DHKPKLTGMV SSMKSNFFAD LPQKLRSKID PENPLHLDVS
KAAGLKEDEK EYYERQLATL KSFEEVESFL ARSDEYTIDE KEEEEDRAER AAQELAMQIS
NWANIFLLAL KIYATVKSGS IAIAASTLDS LLDLMAGGIL WFTHLSMKNV NIYKYPIGKL
RVQPVGIIIF AAVMATLGFQ VLLVAAEQLI SNEPSEKMNH VQLIWLYSIM LSATAIKLVL
WIYCKSSRNH IVRAYAKDHH FDVVTNVLGL VAAVLANAFY WWLDPTGAIL LAIYTIVNWS
GTVMENAVSL IGQSAPPEVL QKLTYLVMRQ GGDNIKHVDT VRAYTFGVLY FVEVDIELPE
DLPLKEAHAI GESLQIKLEE LPEVERAFVH LDFECHHKPE HSVLSTIPND L
