MTPC4_ARATH
ID MTPC4_ARATH Reviewed; 457 AA.
AC Q8H1G3; Q949W1; Q9C531;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Metal tolerance protein C4;
DE Short=AtMTPc4;
DE AltName: Full=AtMTP7;
GN Name=MTPC4; Synonyms=MTP7; OrderedLocusNames=At1g51610;
GN ORFNames=F19C24.16, F5D21.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
CC -!- FUNCTION: Involved in sequestration of excess metal in the cytoplasm
CC into vacuoles to maintain metal homeostasis. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Note=Tonoplast.
CC -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC transporter (TC 2.A.4) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG50870.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAG52617.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC024261; AAG52617.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC025294; AAG50870.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE32690.1; -; Genomic_DNA.
DR EMBL; AY050856; AAK92793.1; -; mRNA.
DR EMBL; AY150383; AAN12928.1; -; mRNA.
DR PIR; G96554; G96554.
DR RefSeq; NP_564594.1; NM_104039.4.
DR AlphaFoldDB; Q8H1G3; -.
DR STRING; 3702.AT1G51610.1; -.
DR TCDB; 2.A.4.6.2; the cation diffusion facilitator (cdf) family.
DR iPTMnet; Q8H1G3; -.
DR PaxDb; Q8H1G3; -.
DR PRIDE; Q8H1G3; -.
DR ProteomicsDB; 250991; -.
DR EnsemblPlants; AT1G51610.1; AT1G51610.1; AT1G51610.
DR GeneID; 841586; -.
DR Gramene; AT1G51610.1; AT1G51610.1; AT1G51610.
DR KEGG; ath:AT1G51610; -.
DR Araport; AT1G51610; -.
DR TAIR; locus:2017562; AT1G51610.
DR eggNOG; KOG2802; Eukaryota.
DR HOGENOM; CLU_021126_1_1_1; -.
DR InParanoid; Q8H1G3; -.
DR OMA; GIQNLWT; -.
DR OrthoDB; 667718at2759; -.
DR PhylomeDB; Q8H1G3; -.
DR PRO; PR:Q8H1G3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8H1G3; baseline and differential.
DR Genevisible; Q8H1G3; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006829; P:zinc ion transport; IEA:InterPro.
DR Gene3D; 1.20.1510.10; -; 1.
DR InterPro; IPR002524; Cation_efflux.
DR InterPro; IPR027469; Cation_efflux_TMD_sf.
DR InterPro; IPR040177; SLC30A9.
DR PANTHER; PTHR13414; PTHR13414; 1.
DR Pfam; PF01545; Cation_efflux; 1.
DR SUPFAM; SSF161111; SSF161111; 1.
DR TIGRFAMs; TIGR01297; CDF; 1.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport; Vacuole.
FT CHAIN 1..457
FT /note="Metal tolerance protein C4"
FT /id="PRO_0000206123"
FT TOPO_DOM 1..115
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 137..141
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 163..183
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 205..220
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 242..267
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 289..297
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 319..457
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 435
FT /note="E -> G (in Ref. 3; AAK92793)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 457 AA; 50104 MW; BDCBDF16A6F37BE0 CRC64;
MQSSHRILSR LLHSPRKGYI RASVGGSVHF LALFDEKDNG FVDTTHRSFS SLIRSSSHVR
GLISTNCLNK GLGVRCSVSL DRETPLIDTY SSHRNFFTRA KQVKRIEIND QHSQRAVTTA
LWCNFLVFSL KFGVWWTSSS HVIMAEVVHS VADFANQALL AYGLSSSRRA PDALHPYGYS
KERFVWSLIS AVGIFCLGSG ATIVNGVQNL WTSSPPPNME LAAVVIGGSF LIEGASLLVA
IQSVKKGAAQ EGMTIRDYIW RGHDPTSVAV MTEDGAAVAG LAIAAASLVA VRMTGNPIYD
PIGSIVVGNL LGMVAIFLIQ RNRHALIGRA MDDQDMSKVL KFLRNDSVVD SLYDCKSEVI
GPGSFRFKAE IDFNGQMVVQ NYLKRTGREE WAKMFREAAK NGDDSAMLNI MSNYGEEVVT
ALGSEVDRLE KEIQELVPGI QHVDIEAHNP TPTDPSL
