MTR2_YEAST
ID MTR2_YEAST Reviewed; 184 AA.
AC P34232; D6VX14;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=mRNA transport regulator MTR2;
GN Name=MTR2; OrderedLocusNames=YKL186C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7865887; DOI=10.1091/mbc.5.11.1253;
RA Kadowaki T., Hitomi M., Chen S., Tartakoff A.M.;
RT "Nuclear mRNA accumulation causes nucleolar fragmentation in yeast mtr2
RT mutant.";
RL Mol. Biol. Cell 5:1253-1263(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8154185; DOI=10.1002/yea.320091208;
RA Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J.,
RA Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W.;
RT "Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI
RT from Saccharomyces cerevisiae reveals 23 open reading frames including the
RT FAS1 gene.";
RL Yeast 9:1343-1348(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-125, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-125, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 14-178 IN COMPLEX WITH MEX67.
RX PubMed=12835756; DOI=10.1038/sj.embor.embor883;
RA Fribourg S., Conti E.;
RT "Structural similarity in the absence of sequence homology of the messenger
RT RNA export factors Mtr2 and p15.";
RL EMBO Rep. 4:699-703(2003).
CC -!- FUNCTION: Affects mRNA transport from the nucleus to the cytoplasm.
CC -!- SUBUNIT: Interacts with MEX67. {ECO:0000269|PubMed:12835756}.
CC -!- INTERACTION:
CC P34232; Q99257: MEX67; NbExp=2; IntAct=EBI-11585, EBI-11642;
CC -!- SUBCELLULAR LOCATION: Nucleus.
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DR EMBL; S77467; AAB33484.1; -; Genomic_DNA.
DR EMBL; X74151; CAA52252.1; -; Genomic_DNA.
DR EMBL; Z28186; CAA82029.1; -; Genomic_DNA.
DR EMBL; AY558555; AAS56881.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA08980.1; -; Genomic_DNA.
DR PIR; S34684; S34684.
DR RefSeq; NP_012735.1; NM_001179752.1.
DR PDB; 1OF5; X-ray; 2.80 A; B=1-184.
DR PDB; 4WWU; X-ray; 3.30 A; C/F/I/L=1-184.
DR PDBsum; 1OF5; -.
DR PDBsum; 4WWU; -.
DR AlphaFoldDB; P34232; -.
DR SMR; P34232; -.
DR BioGRID; 33936; 154.
DR ComplexPortal; CPX-1142; mRNA nuclear export factor complex, MEX67-MTR2.
DR DIP; DIP-4856N; -.
DR IntAct; P34232; 8.
DR MINT; P34232; -.
DR STRING; 4932.YKL186C; -.
DR TCDB; 1.I.1.1.1; the nuclear pore complex (npc) family.
DR iPTMnet; P34232; -.
DR MaxQB; P34232; -.
DR PaxDb; P34232; -.
DR PRIDE; P34232; -.
DR EnsemblFungi; YKL186C_mRNA; YKL186C; YKL186C.
DR GeneID; 853649; -.
DR KEGG; sce:YKL186C; -.
DR SGD; S000001669; MTR2.
DR VEuPathDB; FungiDB:YKL186C; -.
DR eggNOG; ENOG502RZK7; Eukaryota.
DR HOGENOM; CLU_128326_0_0_1; -.
DR InParanoid; P34232; -.
DR OMA; FDCHLIP; -.
DR BioCyc; YEAST:G3O-31949-MON; -.
DR EvolutionaryTrace; P34232; -.
DR PRO; PR:P34232; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P34232; protein.
DR GO; GO:0044613; C:nuclear pore central transport channel; IBA:GO_Central.
DR GO; GO:0042272; C:nuclear RNA export factor complex; IPI:SGD.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0006406; P:mRNA export from nucleus; IC:ComplexPortal.
DR GO; GO:0006913; P:nucleocytoplasmic transport; IBA:GO_Central.
DR GO; GO:0016973; P:poly(A)+ mRNA export from nucleus; IMP:SGD.
DR GO; GO:0006606; P:protein import into nucleus; IBA:GO_Central.
DR GO; GO:0000055; P:ribosomal large subunit export from nucleus; IMP:SGD.
DR GO; GO:0000056; P:ribosomal small subunit export from nucleus; IDA:ComplexPortal.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR045875; NTF2/Mtr2.
DR InterPro; IPR019488; Nucl_pore_RNA_shuttling_Mtr2.
DR PANTHER; PTHR12612; PTHR12612; 1.
DR Pfam; PF10429; Mtr2; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Nucleus; Phosphoprotein; Reference proteome; Transport.
FT CHAIN 1..184
FT /note="mRNA transport regulator MTR2"
FT /id="PRO_0000096634"
FT REGION 111..135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 117..132
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 125
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
FT HELIX 15..31
FT /evidence="ECO:0007829|PDB:1OF5"
FT HELIX 39..42
FT /evidence="ECO:0007829|PDB:1OF5"
FT STRAND 47..49
FT /evidence="ECO:0007829|PDB:4WWU"
FT STRAND 52..54
FT /evidence="ECO:0007829|PDB:1OF5"
FT STRAND 57..60
FT /evidence="ECO:0007829|PDB:1OF5"
FT HELIX 62..72
FT /evidence="ECO:0007829|PDB:1OF5"
FT STRAND 77..88
FT /evidence="ECO:0007829|PDB:1OF5"
FT TURN 89..92
FT /evidence="ECO:0007829|PDB:1OF5"
FT STRAND 93..103
FT /evidence="ECO:0007829|PDB:1OF5"
FT STRAND 115..117
FT /evidence="ECO:0007829|PDB:4WWU"
FT STRAND 145..154
FT /evidence="ECO:0007829|PDB:1OF5"
FT HELIX 155..159
FT /evidence="ECO:0007829|PDB:1OF5"
FT STRAND 166..175
FT /evidence="ECO:0007829|PDB:1OF5"
SQ SEQUENCE 184 AA; 20784 MW; D3AE487A11FA2750 CRC64;
MNTNSNTMVM NDANQAQITA TFTKKILAHL DDPDSNKLAQ FVQLFNPNNC RIIFNATPFA
QATVFLQMWQ NQVVQTQHAL TGVDYHAIPG SGTLICNVNC KVRFDESGRD KMGQDATVPI
QPNNTGNRNR PNDMNKPRPL WGPYFGISLQ LIIDDRIFRN DFNGVISGFN YNMVYKPEDS
LLKI
