ID   MTR49_DEIRA             Reviewed;         408 AA.
AC   Q9RYA1;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase DR_0049 {ECO:0000305};
DE            EC=2.1.1.- {ECO:0000269|PubMed:26590840};
DE   AltName: Full=23S rRNA m5C2499 methyltransferase {ECO:0000305};
GN   OrderedLocusNames=DR_0049 {ECO:0000312|EMBL:AAF09641.1};
OS   Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG
OS   4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422).
OC   Bacteria; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae;
OC   Deinococcus.
OX   NCBI_TaxID=243230;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB
RC   9279 / R1 / VKM B-1422;
RX   PubMed=10567266; DOI=10.1126/science.286.5444.1571;
RA   White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D.,
RA   Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L.,
RA   Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M.,
RA   Vamathevan J.J., Lam P., McDonald L.A., Utterback T.R., Zalewski C.,
RA   Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D.,
RA   Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C.,
RA   Fraser C.M.;
RT   "Genome sequence of the radioresistant bacterium Deinococcus radiodurans
RT   R1.";
RL   Science 286:1571-1577(1999).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB
RC   9279 / R1 / VKM B-1422;
RX   PubMed=26590840; DOI=10.1007/s00792-015-0800-z;
RA   Mundus J., Flyvbjerg K.F., Kirpekar F.;
RT   "Identification of the methyltransferase targeting C2499 in Deinococcus
RT   radiodurans 23S ribosomal RNA.";
RL   Extremophiles 20:91-99(2016).
CC   -!- FUNCTION: Specifically methylates the cytosine at position 2499
CC       (m5C2499) of 23S rRNA. {ECO:0000269|PubMed:26590840}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cytidine(2499) in 23S rRNA + S-adenosyl-L-methionine = 5-
CC         methylcytidine(2499) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:62012, Rhea:RHEA-COMP:16008, Rhea:RHEA-COMP:16009,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74483, ChEBI:CHEBI:82748;
CC         Evidence={ECO:0000269|PubMed:26590840};
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE000513; AAF09641.1; -; Genomic_DNA.
DR   PIR; G75565; G75565.
DR   RefSeq; NP_293775.1; NC_001263.1.
DR   RefSeq; WP_010886697.1; NZ_CP015081.1.
DR   AlphaFoldDB; Q9RYA1; -.
DR   SMR; Q9RYA1; -.
DR   STRING; 243230.DR_0049; -.
DR   EnsemblBacteria; AAF09641; AAF09641; DR_0049.
DR   KEGG; dra:DR_0049; -.
DR   PATRIC; fig|243230.17.peg.214; -.
DR   eggNOG; COG1092; Bacteria.
DR   HOGENOM; CLU_014042_0_0_0; -.
DR   InParanoid; Q9RYA1; -.
DR   OMA; DCFCHTG; -.
DR   OrthoDB; 468283at2; -.
DR   Proteomes; UP000002524; Chromosome I.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.130.10; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR015947; PUA-like_sf.
DR   InterPro; IPR036974; PUA_sf.
DR   InterPro; IPR041532; RlmI_PUA-like.
DR   InterPro; IPR019614; SAM-dep_methyl-trfase.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF10672; Methyltrans_SAM; 1.
DR   Pfam; PF17785; PUA_3; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   SUPFAM; SSF88697; SSF88697; 1.
PE   1: Evidence at protein level;
KW   Methyltransferase; Reference proteome; RNA-binding; rRNA processing;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..408
FT                   /note="Ribosomal RNA large subunit methyltransferase
FT                   DR_0049"
FT                   /id="PRO_0000448943"
SQ   SEQUENCE   408 AA;  45007 MW;  9DBB6185085D0AFC CRC64;
     MSAPASSAPR LRLRVSKAAE LHIRDGHPWV YESSVREQNR EGEPGELAVV YDRRDRFLAI
     GLYDPFSPLR LRVLHTGMPT QLDDAWWAAR LDAALARRAA LFGPLTAFGD TDGYRVLNGE
     SDGFPGLVVD RYAGVLVMKL YTAAWFPHLR RMLELFAARA PDFAVVLRLS RNIAERAADL
     GLHDGQVIYG ELAGDSVVFR ESGLRFEAEV RQGQKTGFFL DQRENRRRVE GLSEGRRVLN
     AFSFSGGFSL YAARGGASEV TSLDISAHAL RSAERNFALN PELSAVHKTV QADVFEWLPA
     GKGSGADYDL VILDPPSLAR REAEREGAIR AYGKLAEGGL TRLAPGGILV SASCSAHVSA
     EEFEEAVMSA VRRSGRRWRK LLSSRHAPDH HASFAEAEYL KAVFLQMD