ID   MTRA_METFA              Reviewed;         241 AA.
AC   C7P879;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   13-OCT-2009, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Tetrahydromethanopterin S-methyltransferase subunit A {ECO:0000255|HAMAP-Rule:MF_01093};
DE            EC=2.1.1.86 {ECO:0000255|HAMAP-Rule:MF_01093};
DE   AltName: Full=N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit A {ECO:0000255|HAMAP-Rule:MF_01093};
GN   Name=mtrA {ECO:0000255|HAMAP-Rule:MF_01093}; OrderedLocusNames=Mefer_0943;
OS   Methanocaldococcus fervens (strain DSM 4213 / JCM 15782 / AG86)
OS   (Methanococcus fervens).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=573064;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 4213 / JCM 15782 / AG86;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D.,
RA   Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.,
RA   Lupa-Sieprawska M., Whitman W.B.;
RT   "Complete sequence of chromosome of Methanocaldococcus fervens AG86.";
RL   Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Part of a complex that catalyzes the formation of methyl-
CC       coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-
CC       tetrahydromethanopterin. This is an energy-conserving, sodium-ion
CC       translocating step. {ECO:0000255|HAMAP-Rule:MF_01093}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2
CC         Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2
CC         Na(+)(out); Xref=Rhea:RHEA:53492, ChEBI:CHEBI:29101,
CC         ChEBI:CHEBI:58103, ChEBI:CHEBI:58116, ChEBI:CHEBI:58286,
CC         ChEBI:CHEBI:58319; EC=2.1.1.86; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01093};
CC   -!- COFACTOR:
CC       Name=5-hydroxybenzimidazolylcob(I)amide; Xref=ChEBI:CHEBI:60494;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01093};
CC       Note=Binds 1 5-hydroxybenzimidazolylcobamide group. {ECO:0000255|HAMAP-
CC       Rule:MF_01093};
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); methyl-
CC       coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step
CC       2/2. {ECO:0000255|HAMAP-Rule:MF_01093}.
CC   -!- SUBUNIT: The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD,
CC       MtrE, MtrF, MtrG and MtrH. {ECO:0000255|HAMAP-Rule:MF_01093}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01093};
CC       Single-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01093}.
CC   -!- SIMILARITY: Belongs to the MtrA family. {ECO:0000255|HAMAP-
CC       Rule:MF_01093}.
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DR   EMBL; CP001696; ACV24761.1; -; Genomic_DNA.
DR   RefSeq; WP_015791498.1; NC_013156.1.
DR   AlphaFoldDB; C7P879; -.
DR   SMR; C7P879; -.
DR   STRING; 573064.Mefer_0943; -.
DR   EnsemblBacteria; ACV24761; ACV24761; Mefer_0943.
DR   GeneID; 8365633; -.
DR   KEGG; mfe:Mefer_0943; -.
DR   eggNOG; arCOG03221; Archaea.
DR   HOGENOM; CLU_100863_0_0_2; -.
DR   OMA; EVKGHIT; -.
DR   OrthoDB; 89197at2157; -.
DR   UniPathway; UPA00640; UER00698.
DR   Proteomes; UP000001495; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0050897; F:cobalt ion binding; IEA:InterPro.
DR   GO; GO:0030269; F:tetrahydromethanopterin S-methyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006814; P:sodium ion transport; IEA:InterPro.
DR   HAMAP; MF_01093; MtrA; 1.
DR   InterPro; IPR030688; MeTrfase_MtrA/MtxA.
DR   InterPro; IPR005778; MtrA.
DR   Pfam; PF04208; MtrA; 1.
DR   PIRSF; PIRSF500207; MtrA; 1.
DR   PIRSF; PIRSF009452; MtrA_MtxA; 1.
DR   TIGRFAMs; TIGR01111; mtrA; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cobalt; Membrane; Methanogenesis; Methyltransferase;
KW   One-carbon metabolism; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..241
FT                   /note="Tetrahydromethanopterin S-methyltransferase subunit
FT                   A"
FT                   /id="PRO_0000403061"
FT   TOPO_DOM        1..220
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01093"
FT   TRANSMEM        221..241
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01093"
FT   BINDING         85
FT                   /ligand="5-hydroxybenzimidazolylcob(I)amide"
FT                   /ligand_id="ChEBI:CHEBI:60494"
FT                   /ligand_note="cofactor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01093"
SQ   SEQUENCE   241 AA;  25590 MW;  B1005D145EA2003E CRC64;
     MANKKSPAPG WPIVSGEYVV GNPESCVGVV TLGSHGLEQA CIDAGAAIVG PCHTENLGIE
     KIIANYISNP NIRFMVLCGS EVQGHLTGQC LKALWENGIG DDGGIIGAKG AIPFLENVNK
     EAVERFRRQI VEVVDLIDCE DVNKITQAIK ECISKDPGAI DEEPIVLELE SGGEGTEEEG
     ATVKPTSPEI ALLEARMRIL SEKINSSALL AKYNSGYYNG KIQGIAIGLF VSILLFSLLG
     I