ID   MTRB_METMA              Reviewed;         108 AA.
AC   P80655; O59639;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 4.
DT   25-MAY-2022, entry version 143.
DE   RecName: Full=Tetrahydromethanopterin S-methyltransferase subunit B;
DE            EC=2.1.1.86;
DE   AltName: Full=N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit B;
GN   Name=mtrB; OrderedLocusNames=MM_1544;
OS   Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM
OS   11833 / OCM 88) (Methanosarcina frisia).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=192952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=9559648; DOI=10.1016/s0014-5793(98)00229-4;
RA   Lienard T., Gottschalk G.;
RT   "Cloning, sequencing and expression of the genes encoding the sodium
RT   translocating N5-methyltetrahydromethanopterin:coenzyme M methyltransferase
RT   of the methylotrophic archaeon Methanosarcina mazei Go1.";
RL   FEBS Lett. 425:204-208(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=12125824;
RA   Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA   Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA   Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA   Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA   Fritz H.-J., Gottschalk G.;
RT   "The genome of Methanosarcina mazei: evidence for lateral gene transfer
RT   between Bacteria and Archaea.";
RL   J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
RN   [3]
RP   PROTEIN SEQUENCE OF 2-16.
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=8774736; DOI=10.1111/j.1432-1033.1996.0857u.x;
RA   Lienard T., Becher B., Marschall M., Bowien S., Gottschalk G.;
RT   "Sodium ion translocation by N5-methyltetrahydromethanopterin: coenzyme M
RT   methyltransferase from Methanosarcina mazei Go1 reconstituted in ether
RT   lipid liposomes.";
RL   Eur. J. Biochem. 239:857-864(1996).
CC   -!- FUNCTION: Part of a complex that catalyzes the formation of methyl-
CC       coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-
CC       tetrahydromethanopterin. This is an energy-conserving, sodium-ion
CC       translocating step.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2
CC         Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2
CC         Na(+)(out); Xref=Rhea:RHEA:53492, ChEBI:CHEBI:29101,
CC         ChEBI:CHEBI:58103, ChEBI:CHEBI:58116, ChEBI:CHEBI:58286,
CC         ChEBI:CHEBI:58319; EC=2.1.1.86;
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); methyl-
CC       coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step
CC       2/2.
CC   -!- SUBUNIT: The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD,
CC       MtrE, MtrF, MtrG and MtrH. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the MtrB family. {ECO:0000305}.
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DR   EMBL; AF042381; AAC38333.1; -; Genomic_DNA.
DR   EMBL; AE008384; AAM31240.1; -; Genomic_DNA.
DR   RefSeq; WP_011033490.1; NC_003901.1.
DR   AlphaFoldDB; P80655; -.
DR   STRING; 192952.MM_1544; -.
DR   EnsemblBacteria; AAM31240; AAM31240; MM_1544.
DR   GeneID; 24879461; -.
DR   KEGG; mma:MM_1544; -.
DR   PATRIC; fig|192952.21.peg.1785; -.
DR   eggNOG; arCOG04867; Archaea.
DR   HOGENOM; CLU_171544_1_0_2; -.
DR   OMA; TNMVYGF; -.
DR   BRENDA; 2.1.1.86; 3270.
DR   UniPathway; UPA00640; UER00698.
DR   Proteomes; UP000000595; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030269; F:tetrahydromethanopterin S-methyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01094; MtrB; 1.
DR   InterPro; IPR008690; MtrB_MeTrfase.
DR   Pfam; PF05440; MtrB; 1.
DR   PIRSF; PIRSF005518; MtrB; 1.
DR   TIGRFAMs; TIGR04166; methano_MtrB; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Direct protein sequencing; Membrane; Methanogenesis;
KW   Methyltransferase; One-carbon metabolism; Reference proteome; Transferase;
KW   Transmembrane; Transmembrane helix.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8774736"
FT   CHAIN           2..108
FT                   /note="Tetrahydromethanopterin S-methyltransferase subunit
FT                   B"
FT                   /id="PRO_0000147516"
FT   TRANSMEM        81..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        20
FT                   /note="T -> N (in Ref. 1; AAC38333)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        104..108
FT                   /note="Missing (in Ref. 1; AAC38333)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   108 AA;  11872 MW;  4D8ABA38776252E5 CRC64;
     MSIVRIAPEI NLVMDTESGT VTQERKDSIQ YSMEPVFERV DKLDAIADDL VNSLSPSKPL
     LNTWPGRENT SYIAGIYSNS FYGIIVGLAF SGLLALIIYI TRLMGGVV