MTRD_METAC
ID MTRD_METAC Reviewed; 249 AA.
AC Q8TU00;
DT 28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=Tetrahydromethanopterin S-methyltransferase subunit D {ECO:0000255|HAMAP-Rule:MF_01097};
DE EC=2.1.1.86 {ECO:0000255|HAMAP-Rule:MF_01097};
DE AltName: Full=N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit D {ECO:0000255|HAMAP-Rule:MF_01097};
GN Name=mtrD {ECO:0000255|HAMAP-Rule:MF_01097}; OrderedLocusNames=MA_0275;
OS Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS C2A).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=188937;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX PubMed=11932238; DOI=10.1101/gr.223902;
RA Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT physiological diversity.";
RL Genome Res. 12:532-542(2002).
CC -!- FUNCTION: Part of a complex that catalyzes the formation of methyl-
CC coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-
CC tetrahydromethanopterin. This is an energy-conserving, sodium-ion
CC translocating step. {ECO:0000255|HAMAP-Rule:MF_01097}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2
CC Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2
CC Na(+)(out); Xref=Rhea:RHEA:53492, ChEBI:CHEBI:29101,
CC ChEBI:CHEBI:58103, ChEBI:CHEBI:58116, ChEBI:CHEBI:58286,
CC ChEBI:CHEBI:58319; EC=2.1.1.86; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01097};
CC -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); methyl-
CC coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step
CC 2/2. {ECO:0000255|HAMAP-Rule:MF_01097}.
CC -!- SUBUNIT: The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD,
CC MtrE, MtrF, MtrG and MtrH. {ECO:0000255|HAMAP-Rule:MF_01097}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01097};
CC Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01097}.
CC -!- SIMILARITY: Belongs to the MtrD family. {ECO:0000255|HAMAP-
CC Rule:MF_01097}.
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DR EMBL; AE010299; AAM03728.1; -; Genomic_DNA.
DR RefSeq; WP_011020333.1; NC_003552.1.
DR AlphaFoldDB; Q8TU00; -.
DR STRING; 188937.MA_0275; -.
DR EnsemblBacteria; AAM03728; AAM03728; MA_0275.
DR GeneID; 1472167; -.
DR KEGG; mac:MA_0275; -.
DR HOGENOM; CLU_1109510_0_0_2; -.
DR InParanoid; Q8TU00; -.
DR OMA; HDPKFKR; -.
DR OrthoDB; 95538at2157; -.
DR PhylomeDB; Q8TU00; -.
DR UniPathway; UPA00640; UER00698.
DR Proteomes; UP000002487; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0012506; C:vesicle membrane; IEA:InterPro.
DR GO; GO:0030269; F:tetrahydromethanopterin S-methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006814; P:sodium ion transport; IEA:InterPro.
DR HAMAP; MF_01097; MtrD; 1.
DR InterPro; IPR005779; MeTrfase_D.
DR Pfam; PF04207; MtrD; 1.
DR PIRSF; PIRSF016552; MtrD; 1.
DR TIGRFAMs; TIGR01112; mtrD; 1.
PE 3: Inferred from homology;
KW Cell membrane; Membrane; Methanogenesis; Methyltransferase;
KW One-carbon metabolism; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..249
FT /note="Tetrahydromethanopterin S-methyltransferase subunit
FT D"
FT /id="PRO_0000147528"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01097"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01097"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01097"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01097"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01097"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01097"
SQ SEQUENCE 249 AA; 25352 MW; 85A9E4CAA18B808D CRC64;
MIDALMANIL WLVFIIIGGV LISWSVHFVP VGGAPAAMAQ ATGIGTGTVQ LAAGAGLTGL
VSAGFMMNVT DNLPLILASG AVGAMIMISV TMIVGTWVYV YGVGCVPSSA KVKYDPITKY
RQDLYVSQGT EGHGLPTVSF VSGVIGGLLG GIGGALVYYS LIEVGLTAGL STGTSSGVTG
HELVGIAAMF AIGIFFVNAV IPSYNIGGTI EGFHDPKWKK WPKAVISSFV ATILCAIVAV
IAISQLGGI
