MTRE_METMA
ID MTRE_METMA Reviewed; 304 AA.
AC P80651; O59636;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1999, sequence version 2.
DT 25-MAY-2022, entry version 138.
DE RecName: Full=Tetrahydromethanopterin S-methyltransferase subunit E;
DE EC=2.1.1.86;
DE AltName: Full=N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit E;
GN Name=mtrE; OrderedLocusNames=MM_1547;
OS Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM
OS 11833 / OCM 88) (Methanosarcina frisia).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=192952;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX PubMed=9559648; DOI=10.1016/s0014-5793(98)00229-4;
RA Lienard T., Gottschalk G.;
RT "Cloning, sequencing and expression of the genes encoding the sodium
RT translocating N5-methyltetrahydromethanopterin:coenzyme M methyltransferase
RT of the methylotrophic archaeon Methanosarcina mazei Go1.";
RL FEBS Lett. 425:204-208(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX PubMed=12125824;
RA Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA Fritz H.-J., Gottschalk G.;
RT "The genome of Methanosarcina mazei: evidence for lateral gene transfer
RT between Bacteria and Archaea.";
RL J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
RN [3]
RP PROTEIN SEQUENCE OF 1-15.
RC STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX PubMed=8774736; DOI=10.1111/j.1432-1033.1996.0857u.x;
RA Lienard T., Becher B., Marschall M., Bowien S., Gottschalk G.;
RT "Sodium ion translocation by N5-methyltetrahydromethanopterin: coenzyme M
RT methyltransferase from Methanosarcina mazei Go1 reconstituted in ether
RT lipid liposomes.";
RL Eur. J. Biochem. 239:857-864(1996).
CC -!- FUNCTION: Part of a complex that catalyzes the formation of methyl-
CC coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-
CC tetrahydromethanopterin. This is an energy-conserving, sodium-ion
CC translocating step.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2
CC Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2
CC Na(+)(out); Xref=Rhea:RHEA:53492, ChEBI:CHEBI:29101,
CC ChEBI:CHEBI:58103, ChEBI:CHEBI:58116, ChEBI:CHEBI:58286,
CC ChEBI:CHEBI:58319; EC=2.1.1.86;
CC -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); methyl-
CC coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step
CC 2/2.
CC -!- SUBUNIT: The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD,
CC MtrE, MtrF, MtrG and MtrH. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the MtrE family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF042381; AAC38330.1; -; Genomic_DNA.
DR EMBL; AE008384; AAM31243.1; -; Genomic_DNA.
DR RefSeq; WP_011033493.1; NC_003901.1.
DR AlphaFoldDB; P80651; -.
DR STRING; 192952.MM_1547; -.
DR EnsemblBacteria; AAM31243; AAM31243; MM_1547.
DR GeneID; 24840897; -.
DR KEGG; mma:MM_1547; -.
DR PATRIC; fig|192952.21.peg.1788; -.
DR eggNOG; arCOG04870; Archaea.
DR HOGENOM; CLU_958513_0_0_2; -.
DR OMA; QMGNIHR; -.
DR BRENDA; 2.1.1.86; 3270.
DR UniPathway; UPA00640; UER00698.
DR Proteomes; UP000000595; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0012506; C:vesicle membrane; IEA:InterPro.
DR GO; GO:0030269; F:tetrahydromethanopterin S-methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006814; P:sodium ion transport; IEA:InterPro.
DR HAMAP; MF_01098; MtrE; 1.
DR InterPro; IPR005780; MeTrfase_E.
DR Pfam; PF04206; MtrE; 1.
DR PIRSF; PIRSF016509; MtrE; 1.
DR TIGRFAMs; TIGR01113; mtrE; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Direct protein sequencing; Membrane; Methanogenesis;
KW Methyltransferase; One-carbon metabolism; Reference proteome; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..304
FT /note="Tetrahydromethanopterin S-methyltransferase subunit
FT E"
FT /id="PRO_0000147541"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 259..279
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 2
FT /note="E -> D (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 4
FT /note="L -> R (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 7
FT /note="M -> A (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 9
FT /note="V -> G (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 12
FT /note="L -> I (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 304 AA; 32489 MW; E1741B8BF21B07F7 CRC64;
MEPLIGMGVL ALIGVAATIA GASEDLESDI GSQSNPNSQV QLAPQMMFPH RIFNKAISGE
PPSNALMCSI GAAIATVLIS EFTVSPLFAL VFGSVIAASV HATFAVTATM GRCASQSRFK
QPIYLDMIRS HTPAIMGYAF ITTFCVLIVS YLMTVVLGHP FPLTMLAFIW GITIGAIGSS
TGDVHYGAER EFQQFEFGSG LNASNSGNIV RYAESGLRNG FDNSWFCSKF GGPTTGIAFG
MTVFLGSWIT TIFDPAQGLS MGWLSVIAGV IIVLILIIWN WKIEVQARKA YGPYKEDKAE
EASA
