MTRF1_ARATH
ID MTRF1_ARATH Reviewed; 413 AA.
AC A2RVR7; O80723; Q0WSC9; Q304B8;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Peptide chain release factor 1, mitochondrial {ECO:0000305};
DE Short=AtmtRF1 {ECO:0000305};
DE Flags: Precursor;
GN OrderedLocusNames=At2g47020 {ECO:0000312|Araport:AT2G47020};
GN ORFNames=F14M4.15 {ECO:0000312|EMBL:AAC34223.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia, and cv. No-0;
RX PubMed=17450416; DOI=10.1007/s11103-007-9166-7;
RA Motohashi R., Yamazaki T., Myouga F., Ito T., Ito K., Satou M.,
RA Kobayashi M., Nagata N., Yoshida S., Nagashima A., Tanaka K., Takahashi S.,
RA Shinozaki K.;
RT "Chloroplast ribosome release factor 1 (AtcpRF1) is essential for
RT chloroplast development.";
RL Plant Mol. Biol. 64:481-497(2007).
CC -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC translation in response to the peptide chain termination codons UAG and
CC UAA in mitochondria. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A2RVR7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A2RVR7-2; Sequence=VSP_060804, VSP_060805, VSP_060806;
CC -!- TISSUE SPECIFICITY: Mostly expressed in seedlings, stems and adult
CC plants, and, to a lower extent, in siliques (PubMed:17450416). Barely
CC detected in etiolated seedlings and roots (PubMed:17450416).
CC {ECO:0000269|PubMed:17450416}.
CC -!- PTM: Methylation increases the termination efficiency of RF1.
CC {ECO:0000250|UniProtKB:P0A7I0}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC34223.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004411; AAC34223.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10789.1; -; Genomic_DNA.
DR EMBL; AK228004; BAE99969.1; -; mRNA.
DR EMBL; BT030058; ABN04796.1; -; mRNA.
DR PIR; T02185; T02185.
DR RefSeq; NP_182225.3; NM_130270.4. [A2RVR7-1]
DR AlphaFoldDB; A2RVR7; -.
DR SMR; A2RVR7; -.
DR STRING; 3702.AT2G47020.1; -.
DR PaxDb; A2RVR7; -.
DR PRIDE; A2RVR7; -.
DR ProteomicsDB; 189205; -. [A2RVR7-1]
DR EnsemblPlants; AT2G47020.1; AT2G47020.1; AT2G47020. [A2RVR7-1]
DR GeneID; 819316; -.
DR Gramene; AT2G47020.1; AT2G47020.1; AT2G47020. [A2RVR7-1]
DR KEGG; ath:AT2G47020; -.
DR Araport; AT2G47020; -.
DR TAIR; locus:2041409; AT2G47020.
DR eggNOG; KOG2726; Eukaryota.
DR HOGENOM; CLU_036856_0_2_1; -.
DR InParanoid; A2RVR7; -.
DR PhylomeDB; A2RVR7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; A2RVR7; baseline and differential.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:InterPro.
DR HAMAP; MF_00093; Rel_fac_1; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004373; RF-1.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR TIGRFAMs; TIGR00019; prfA; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Methylation; Mitochondrion; Protein biosynthesis;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..40
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 41..413
FT /note="Peptide chain release factor 1, mitochondrial"
FT /id="PRO_0000451567"
FT MOD_RES 287
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000250|UniProtKB:P0A7I0"
FT VAR_SEQ 1..75
FT /note="MRVLIRPNFLSNLIRYCSRGTHSHDRSLRSVLSSNMIRLYTTGMEPQLSPDL
FT IKIMDQRLSAIEHRNAVLQKLIN -> MLSFRSLSTR (in isoform 2)"
FT /id="VSP_060804"
FT VAR_SEQ 318..321
FT /note="NRAK -> SQST (in isoform 2)"
FT /id="VSP_060805"
FT VAR_SEQ 322..413
FT /note="Missing (in isoform 2)"
FT /id="VSP_060806"
SQ SEQUENCE 413 AA; 46828 MW; 72756EEFEA7491D3 CRC64;
MRVLIRPNFL SNLIRYCSRG THSHDRSLRS VLSSNMIRLY TTGMEPQLSP DLIKIMDQRL
SAIEHRNAVL QKLINQPEYS PEEFSRANKE LRKLRDSMLL INDLRAKQKE IDGLKSLVSE
SSDDKDMLDL AVGELDEAVE EEKRLQTLLL KSLLPKDEAD ERDCILEVRA GTGGEEASLF
AMDIFRMYER YSQKKGWKFD IVDITESDMK GYKEASAAIC GASVYGKLKF ESGIHRVQRI
PITEKSGRIH TSAISVAILP QADEVDVQLR NEDLRIDTYR SGGSGGQHAN TTNSAVRIIH
LPTGMMVSIQ DERSQHMNRA KALKVLCARL YEIERLRIQS SRSKLRSDQI GSGDRSGRIR
TYNFPQGRVT DHRVGITHHA IEDMMQGENL DMFIDALLLR QEMDAIASFS STS
