ID   MTRH_METKA              Reviewed;         322 AA.
AC   O32869;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2002, sequence version 2.
DT   25-MAY-2022, entry version 122.
DE   RecName: Full=Tetrahydromethanopterin S-methyltransferase subunit H {ECO:0000255|HAMAP-Rule:MF_01501};
DE            EC=2.1.1.86 {ECO:0000255|HAMAP-Rule:MF_01501};
DE   AltName: Full=N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit H {ECO:0000255|HAMAP-Rule:MF_01501};
GN   Name=mtrH {ECO:0000255|HAMAP-Rule:MF_01501}; OrderedLocusNames=MK0662;
OS   Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938).
OC   Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae;
OC   Methanopyrus.
OX   NCBI_TaxID=190192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9461302; DOI=10.1111/j.1432-1033.1997.00783.x;
RA   Harms U., Thauer R.K.;
RT   "Identification of the active site histidine in the corrinoid protein MtrA
RT   of the energy-conserving methyltransferase complex from Methanobacterium
RT   thermoautotrophicum.";
RL   Eur. J. Biochem. 250:783-788(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX   PubMed=11930014; DOI=10.1073/pnas.032671499;
RA   Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N.,
RA   Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A.,
RA   Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G.,
RA   Koonin E.V., Kozyavkin S.A.;
RT   "The complete genome of hyperthermophile Methanopyrus kandleri AV19 and
RT   monophyly of archaeal methanogens.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
CC   -!- FUNCTION: Part of a complex that catalyzes the formation of methyl-
CC       coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-
CC       tetrahydromethanopterin. This is an energy-conserving, sodium-ion
CC       translocating step. MtrH catalyzes the transfer of the methyl group
CC       from methyl-tetrahydromethanopterin to the corrinoid prosthetic group
CC       of MtrA. {ECO:0000255|HAMAP-Rule:MF_01501}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2
CC         Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2
CC         Na(+)(out); Xref=Rhea:RHEA:53492, ChEBI:CHEBI:29101,
CC         ChEBI:CHEBI:58103, ChEBI:CHEBI:58116, ChEBI:CHEBI:58286,
CC         ChEBI:CHEBI:58319; EC=2.1.1.86; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01501};
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); methyl-
CC       coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step
CC       2/2. {ECO:0000255|HAMAP-Rule:MF_01501}.
CC   -!- SUBUNIT: The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD,
CC       MtrE, MtrF, MtrG and MtrH. {ECO:0000255|HAMAP-Rule:MF_01501}.
CC   -!- SIMILARITY: Belongs to the MtrH family. {ECO:0000255|HAMAP-
CC       Rule:MF_01501}.
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DR   EMBL; Y14428; CAA74773.1; -; Genomic_DNA.
DR   EMBL; AE009439; AAM01877.1; -; Genomic_DNA.
DR   AlphaFoldDB; O32869; -.
DR   SMR; O32869; -.
DR   STRING; 190192.MK0662; -.
DR   EnsemblBacteria; AAM01877; AAM01877; MK0662.
DR   KEGG; mka:MK0662; -.
DR   PATRIC; fig|190192.8.peg.701; -.
DR   HOGENOM; CLU_048697_0_0_2; -.
DR   OMA; YARHKIV; -.
DR   UniPathway; UPA00640; UER00698.
DR   Proteomes; UP000001826; Chromosome.
DR   GO; GO:0030269; F:tetrahydromethanopterin S-methyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.20.20.20; -; 1.
DR   HAMAP; MF_01501; MtrH; 1.
DR   InterPro; IPR011005; Dihydropteroate_synth-like.
DR   InterPro; IPR023467; MeTrfase_MtrH/MtxH.
DR   InterPro; IPR028342; MtrH.
DR   Pfam; PF02007; MtrH; 1.
DR   PIRSF; PIRSF500206; MtrH; 1.
DR   PIRSF; PIRSF004960; MtrH_MtxH; 1.
DR   SUPFAM; SSF51717; SSF51717; 1.
DR   TIGRFAMs; TIGR01114; mtrH; 1.
PE   3: Inferred from homology;
KW   Methanogenesis; Methyltransferase; One-carbon metabolism;
KW   Reference proteome; Transferase.
FT   CHAIN           1..322
FT                   /note="Tetrahydromethanopterin S-methyltransferase subunit
FT                   H"
FT                   /id="PRO_0000147564"
FT   CONFLICT        1..4
FT                   /note="MFYY -> MDRCSTT (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        117
FT                   /note="A -> G (in Ref. 1; CAA74773)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   322 AA;  34963 MW;  40661B810E6406EC CRC64;
     MFYYPGKEQK VCDICGVKVG GQPGEYPTVL AGTIFYAGHK IVKDEDKGIF DEEAAEELIK
     MEEELADETG NPMMAHIMGE SEEAIIRYLD WVADVTDAPI IVDSTEAEVK VAAVKHAQEV
     GLAERVVYNS INASVEDEEI QAIKESDCNS AIVLAFNPMD ASVEGRMKIL TEGEEGVSEK
     GMLEISDECG IENPLIDTAY TPFGSGAGTA YKVTLAVKAK LGLPVGGAPH NVPSAWDWLR
     DFMKKLKEEG KEEWAELAHE SSDWGSNVVA ATLCCDYLLF GPIENAPAIW PVVAMVDALI
     VEANEDVGVE PQVEEHPANI VR