MTS1_ORYSJ
ID MTS1_ORYSJ Reviewed; 357 AA.
AC Q6YSY5; B9FWE7;
DT 22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=O-methyltransferase 1, chloroplastic {ECO:0000303|PubMed:29901843};
DE Short=OsMTS1 {ECO:0000303|PubMed:29901843};
DE AltName: Full=Acetylserotonin O-methyltransferase MTS1 {ECO:0000305};
DE EC=2.1.1.4 {ECO:0000269|PubMed:29901843};
DE AltName: Full=Protein LESION AND LAMINA BENDING {ECO:0000303|PubMed:26864209};
DE Short=OsLLB {ECO:0000303|PubMed:26864209};
DE AltName: Full=Protein PREMATURE LEAF SENESCENCE 3 {ECO:0000303|PubMed:29901843};
DE AltName: Full=Putative leucine carboxyl methyltransferase MTS1 {ECO:0000305};
DE Flags: Precursor;
GN Name=MTS1 {ECO:0000303|PubMed:29901843};
GN Synonyms=LLB {ECO:0000303|PubMed:26864209},
GN PLS3 {ECO:0000303|PubMed:29901843};
GN OrderedLocusNames=Os07g0247100 {ECO:0000312|EMBL:BAF21201.1},
GN LOC_Os07g14350 {ECO:0000305};
GN ORFNames=OsJ_23703 {ECO:0000312|EMBL:EEE66884.1},
GN OSJNBa0003K21.34 {ECO:0000312|EMBL:BAC84755.1},
GN P0639B07.8 {ECO:0000312|EMBL:BAD30653.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=26864209; DOI=10.1111/nph.13864;
RA Tamiru M., Takagi H., Abe A., Yokota T., Kanzaki H., Okamoto H., Saitoh H.,
RA Takahashi H., Fujisaki K., Oikawa K., Uemura A., Natsume S., Jikumaru Y.,
RA Matsuura H., Umemura K., Terry M.J., Terauchi R.;
RT "A chloroplast-localized protein LESION AND LAMINA BENDING affects defence
RT and growth responses in rice.";
RL New Phytol. 210:1282-1297(2016).
RN [6]
RP FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=29901843; DOI=10.1111/tpj.13995;
RA Hong Y., Zhang Y., Sinumporn S., Yu N., Zhan X., Shen X., Chen D., Yu P.,
RA Wu W., Liu Q., Cao Z., Zhao C., Cheng S., Cao L.;
RT "Premature leaf senescence 3, encoding a methyltransferase, is required for
RT melatonin biosynthesis in rice.";
RL Plant J. 0:0-0(2018).
CC -!- FUNCTION: Involved in melatonin biosynthesis (PubMed:29901843). Can
CC function as acetylserotonin O-methyltransferase (PubMed:29901843).
CC Catalyzes the transfer of a methyl group onto N-acetylserotonin,
CC producing melatonin (N-acetyl-5-methoxytryptamine) (PubMed:29901843).
CC Involved in the regulation of jasmonate- and brassinosteroid-mediated
CC plant growth and defense responses (PubMed:26864209).
CC {ECO:0000269|PubMed:26864209, ECO:0000269|PubMed:29901843}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N-acetylserotonin + S-adenosyl-L-methionine = H(+) + melatonin
CC + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:15573, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16796, ChEBI:CHEBI:17697, ChEBI:CHEBI:57856,
CC ChEBI:CHEBI:59789; EC=2.1.1.4;
CC Evidence={ECO:0000269|PubMed:29901843};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15574;
CC Evidence={ECO:0000269|PubMed:29901843};
CC -!- PATHWAY: Aromatic compound metabolism; melatonin biosynthesis;
CC melatonin from serotonin: step 1/2. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:26864209, ECO:0000269|PubMed:29901843}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaf sheaths, flag leaves and
CC panicles. {ECO:0000269|PubMed:29901843}.
CC -!- DISRUPTION PHENOTYPE: Reduced plant growth and grain yield
CC (PubMed:26864209, PubMed:29901843). Reduced content of chlorophyll in
CC leaves (PubMed:26864209, PubMed:29901843). Reduced number and size of
CC chloroplasts (PubMed:29901843). Altered structure of chloroplasts
CC (PubMed:29901843). Reduced root length and lateral root number
CC (PubMed:29901843). Formation of hypersensitive response (HR)-like
CC lesions (PubMed:26864209). Early heading and premature leaf senescence
CC (PubMed:29901843). Accumulation of the antimicrobial compounds
CC momilactones and phytocassanes, and constitutive expression of
CC pathogenesis-related genes (PubMed:26864209). Over-accumulation of
CC jasmonates and elevated levels of brassinosteroid (PubMed:26864209).
CC Increased leaf lamina bending (PubMed:26864209).
CC {ECO:0000269|PubMed:26864209, ECO:0000269|PubMed:29901843}.
CC -!- MISCELLANEOUS: Overexpression of MTS1 in transgenic lines enhances
CC grain yield. {ECO:0000269|PubMed:29901843}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. LCMT family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP006266; BAC84755.1; -; Genomic_DNA.
DR EMBL; AP004344; BAD30653.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF21201.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT00815.1; -; Genomic_DNA.
DR EMBL; CM000144; EEE66884.1; -; Genomic_DNA.
DR RefSeq; XP_015645234.1; XM_015789748.1.
DR AlphaFoldDB; Q6YSY5; -.
DR SMR; Q6YSY5; -.
DR STRING; 4530.OS07T0247100-01; -.
DR PaxDb; Q6YSY5; -.
DR PRIDE; Q6YSY5; -.
DR EnsemblPlants; Os07t0247100-01; Os07t0247100-01; Os07g0247100.
DR GeneID; 4342830; -.
DR Gramene; Os07t0247100-01; Os07t0247100-01; Os07g0247100.
DR KEGG; osa:4342830; -.
DR eggNOG; ENOG502QQB5; Eukaryota.
DR HOGENOM; CLU_067447_0_0_1; -.
DR InParanoid; Q6YSY5; -.
DR OMA; ILFVAEH; -.
DR OrthoDB; 1141797at2759; -.
DR UniPathway; UPA00837; UER00815.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000007752; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0017096; F:acetylserotonin O-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0030187; P:melatonin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR007213; Ppm1/Ppm2/Tcmp.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF04072; LCM; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Melatonin biosynthesis; Methyltransferase; Plastid;
KW Reference proteome; S-adenosyl-L-methionine; Transferase; Transit peptide.
FT TRANSIT 1..53
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 54..357
FT /note="O-methyltransferase 1, chloroplastic"
FT /id="PRO_0000449397"
FT REGION 50..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 357 AA; 39828 MW; DE80DA642180BDBF CRC64;
MPVLPWLAAA ATTPVRRSPP LPATPRALLR LPASSFPPWS NCAKSGLPPR GPFATAADTP
LGGSLPEPEE ERDTLLDGAL RAARFRDEES RRPDPLFIDP YAAVLLSLDV ASEDKDLLAL
HLMPSAEHYR LVTRYIDDKL QHFISNSDDL RQIVLLTDGM DTRPYRLSWP RLSVVYDVSP
RRVFITASQQ LRGAGAKISR NCVVLHTSSE SPDLQAGLNK NGFNGNRPSL WVLQGLPLFT
FKSLEDLLLV IGNLAMKGSI FIGEVPRFTQ WGAATDMASE QDRLENLFFT QGFRVSFVHY
EEVAKDVGLG LDSPPEIHGR AIFIAEQLRF SDAQMESFRM HFERIEDDAD EDGFEEL
