ID   MTS30_SELML             Reviewed;         353 AA.
AC   D8S255;
DT   03-APR-2013, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 1.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Microbial Terpene synthase-like protein 30;
DE            Short=SmMTPSL30;
GN   ORFNames=SELMODRAFT_417418;
OS   Selaginella moellendorffii (Spikemoss).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Lycopodiopsida; Selaginellales; Selaginellaceae; Selaginella.
OX   NCBI_TaxID=88036;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=22908266; DOI=10.1073/pnas.1204300109;
RA   Li G., Kollner T.G., Yin Y., Jiang Y., Chen H., Xu Y., Gershenzon J.,
RA   Pichersky E., Chen F.;
RT   "Nonseed plant Selaginella moellendorfii has both seed plant and microbial
RT   types of terpene synthases.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:14711-14715(2012).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=21551031; DOI=10.1126/science.1203810;
RA   Banks J.A., Nishiyama T., Hasebe M., Bowman J.L., Gribskov M.,
RA   dePamphilis C., Albert V.A., Aono N., Aoyama T., Ambrose B.A., Ashton N.W.,
RA   Axtell M.J., Barker E., Barker M.S., Bennetzen J.L., Bonawitz N.D.,
RA   Chapple C., Cheng C., Correa L.G., Dacre M., DeBarry J., Dreyer I.,
RA   Elias M., Engstrom E.M., Estelle M., Feng L., Finet C., Floyd S.K.,
RA   Frommer W.B., Fujita T., Gramzow L., Gutensohn M., Harholt J., Hattori M.,
RA   Heyl A., Hirai T., Hiwatashi Y., Ishikawa M., Iwata M., Karol K.G.,
RA   Koehler B., Kolukisaoglu U., Kubo M., Kurata T., Lalonde S., Li K., Li Y.,
RA   Litt A., Lyons E., Manning G., Maruyama T., Michael T.P., Mikami K.,
RA   Miyazaki S., Morinaga S., Murata T., Mueller-Roeber B., Nelson D.R.,
RA   Obara M., Oguri Y., Olmstead R.G., Onodera N., Petersen B.L., Pils B.,
RA   Prigge M., Rensing S.A., Riano-Pachon D.M., Roberts A.W., Sato Y.,
RA   Scheller H.V., Schulz B., Schulz C., Shakirov E.V., Shibagaki N.,
RA   Shinohara N., Shippen D.E., Soerensen I., Sotooka R., Sugimoto N.,
RA   Sugita M., Sumikawa N., Tanurdzic M., Theissen G., Ulvskov P., Wakazuki S.,
RA   Weng J.K., Willats W.W., Wipf D., Wolf P.G., Yang L., Zimmer A.D., Zhu Q.,
RA   Mitros T., Hellsten U., Loque D., Otillar R., Salamov A., Schmutz J.,
RA   Shapiro H., Lindquist E., Lucas S., Rokhsar D., Grigoriev I.V.;
RT   "The Selaginella genome identifies genetic changes associated with the
RT   evolution of vascular plants.";
RL   Science 332:960-963(2011).
CC   -!- FUNCTION: No terpene synthase activity detected in vitro.
CC       {ECO:0000269|PubMed:22908266}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
CC   -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC       the catalytic activity, presumably through binding to Mg(2+).
CC       {ECO:0000250}.
CC   -!- MISCELLANEOUS: Selaginella moellendorffii contains two distinct types
CC       of functional terpene synthases (TPS) genes, the typical seed plants
CC       TPS genes (SmTPSs) and a microbial type TPS genes (SmMTPSLs).
CC   -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
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DR   EMBL; JX413789; AFR34009.1; -; mRNA.
DR   EMBL; GL377599; EFJ21571.1; -; Genomic_DNA.
DR   RefSeq; XP_002977567.1; XM_002977521.1.
DR   AlphaFoldDB; D8S255; -.
DR   SMR; D8S255; -.
DR   PRIDE; D8S255; -.
DR   EnsemblPlants; EFJ21571; EFJ21571; SELMODRAFT_417418.
DR   GeneID; 9639225; -.
DR   Gramene; EFJ21571; EFJ21571; SELMODRAFT_417418.
DR   KEGG; smo:SELMODRAFT_417418; -.
DR   eggNOG; ENOG502SHFN; Eukaryota.
DR   HOGENOM; CLU_063343_0_0_1; -.
DR   InParanoid; D8S255; -.
DR   OrthoDB; 1143139at2759; -.
DR   Proteomes; UP000001514; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR034686; Terpene_cyclase-like_2.
DR   SFLD; SFLDG01020; Terpene_Cyclase_Like_2; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   2: Evidence at transcript level;
KW   Magnesium; Metal-binding; Reference proteome.
FT   CHAIN           1..353
FT                   /note="Microbial Terpene synthase-like protein 30"
FT                   /id="PRO_0000421945"
FT   MOTIF           90..94
FT                   /note="DDXXE motif"
FT   BINDING         90
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         90
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         232
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
FT   BINDING         236
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   353 AA;  40525 MW;  F969FBD872156DE9 CRC64;
     MECLMAKLVP RLELPLLPNN IPSACHWDSS SLSQELDQWL ISKLGITDES AKRKIVQSRV
     MLLACLMHPN GERDRVLLAG KHLWVYFLVD DILESSSREG YGALKSIVWS IATTGIYKAS
     EEHDHHDLVL LLLVEVMVEL RKEMPTSLFA RYCKILSIYL DSVQEEVKHQ INNTIPSSEE
     YRLLRRRTGF MEVMACIMTE FCVGINLEEL VVNLGEIREL VKIMDDHIVT VNDLLSLRKE
     YYNGTIYHNW VIVLLAHDCA TFQKSVDRVC EMIKQEEDSI LDLQKKLIIK AKVDKNPELL
     KFAFNVPMAV AGHLKWAFIT ARYHGCDNAL LDGELFHGTW IMDPNQTVIV KNM