MTT1_USTMD
ID MTT1_USTMD Reviewed; 300 AA.
AC A0A0U2IR85;
DT 18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT 16-MAR-2016, sequence version 1.
DT 03-AUG-2022, entry version 18.
DE RecName: Full=Mitochondrial tricarboxylate transporter 1 {ECO:0000303|PubMed:26639528};
DE AltName: Full=Itaconic acid/2-hydroxyparaconate biosynthesis cluster protein MTT1 {ECO:0000305};
GN Name=MTT1 {ECO:0000303|PubMed:26639528}; ORFNames=UMAG_05079;
OS Ustilago maydis (Corn smut fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX NCBI_TaxID=5270;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP SUBCELLULAR LOCATION.
RC STRAIN=MB215;
RX PubMed=26639528; DOI=10.1111/1751-7915.12329;
RA Geiser E., Przybilla S.K., Friedrich A., Buckel W., Wierckx N., Blank L.M.,
RA Boelker M.;
RT "Ustilago maydis produces itaconic acid via the unusual intermediate trans-
RT aconitate.";
RL Microb. Biotechnol. 9:116-126(2016).
RN [2]
RP FUNCTION.
RX PubMed=27750034; DOI=10.1016/j.ymben.2016.10.006;
RA Geiser E., Przybilla S.K., Engel M., Kleineberg W., Buettner L.,
RA Sarikaya E., Hartog T.D., Klankermayer J., Leitner W., Boelker M.,
RA Blank L.M., Wierckx N.;
RT "Genetic and biochemical insights into the itaconate pathway of Ustilago
RT maydis enable enhanced production.";
RL Metab. Eng. 38:427-435(2016).
CC -!- FUNCTION: Mitochondrial tricarboxylate transporter; part of the gene
CC cluster that mediates the biosynthesis of itaconic acid and 2-
CC hydroxyparaconate (PubMed:26639528, PubMed:27750034). Cis-aconitate is
CC secreted by the mitochondrial tricarboxylate transporter MTT1
CC (PubMed:26639528). In the cytosol cis-aconitate is converted into
CC trans-aconitate via isomerization by the aconitate-delta-isomerase ADI1
CC (PubMed:26639528). Decarboxylation of trans-aconitate by the trans-
CC aconitate decarboxylase TAD1 then leads then to the production of
CC itaconic acid (PubMed:26639528). The cytochrome P450 monooxygenase CYP3
CC further converts itaconate to 2-hydroxyparaconate via oxidation of the
CC double bond, leading to a transient epoxide, which can subsequently be
CC lactonized to produce 2-hydroxyparaconate (PubMed:27750034). Secretion
CC of itaconate and possibly 2-hydroxyparaconate into the medium is
CC mediated by the major facilitator ITP1 (PubMed:26639528,
CC PubMed:27750034). The glyoxalase domain-containing protein RDO1 is not
CC involved in the biosynthesis of itaconate and 2-hydroxyparaconate,
CC however, it might play a role in the further conversion of 2-
CC hydroxyparaconate to itatartarate (PubMed:27750034).
CC {ECO:0000269|PubMed:26639528, ECO:0000269|PubMed:27750034}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane
CC {ECO:0000269|PubMed:26639528}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Decreases the production of itaconic acid
CC (PubMed:26639528). {ECO:0000269|PubMed:26639528}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; KT852988; ALS30799.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0U2IR85; -.
DR SMR; A0A0U2IR85; -.
DR VEuPathDB; FungiDB:UMAG_05079; -.
DR OMA; YPTEYLK; -.
DR BioCyc; MetaCyc:MON-20621; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR002067; Mit_carrier.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00926; MITOCARRIER.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 3: Inferred from homology;
KW Membrane; Mitochondrion; Repeat; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..300
FT /note="Mitochondrial tricarboxylate transporter 1"
FT /id="PRO_0000438674"
FT TRANSMEM 11..31
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..191
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 277..297
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 8..98
FT /note="Solcar 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 107..197
FT /note="Solcar 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 209..294
FT /note="Solcar 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
SQ SEQUENCE 300 AA; 31965 MW; 3FD280D6F3E0EEF9 CRC64;
MPPSGRKVSP SVSVVAGATA GAVEGVATFP IEYLKTVSQF APRDVHGNQQ RLSPIEVVRS
TLQKEGPKGL FRGCTAMVVG NAGKAGVRFF AFENFRSMLK NKSTGKLSNS SNYLAGMGAG
TLEAIFAVTP SETIKTKLID DSKRAKPRYE QGLVRGTASI VRQEGLAGIY QGVVPVVMRQ
GSASAIRLGT YSALRDWLPK AHGSGSSLIN WLATFSIGAA SGVVAVYGTM PFDVLKTRMQ
AIDAARYRST WHCLTNTLKT EGAAALWRGS VSRSMRLIVS GGVIFSVYEQ VVWLLAGPES
