MTUS2_MOUSE
ID MTUS2_MOUSE Reviewed; 1353 AA.
AC Q3UHD3; B9EKJ2; Q7TPP7; Q8CHD2; Q9CY74;
DT 06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Microtubule-associated tumor suppressor candidate 2 homolog;
DE AltName: Full=Cardiac zipper protein;
DE AltName: Full=Microtubule plus-end tracking protein TIP150;
DE Short=Tracking protein of 150 kDa;
GN Name=Mtus2; Synonyms=Cazip, Kiaa0774, Tip150;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Placenta;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC TISSUE=Brain, and Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 201-1117 AND 1155-1353 (ISOFORM
RP 1).
RX PubMed=12465718; DOI=10.1093/dnares/9.5.179;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: I.
RT The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 9:179-188(2002).
RN [4]
RP ALTERNATIVE SPLICING, AND TISSUE SPECIFICITY.
RX PubMed=19806667; DOI=10.1002/dvdy.22107;
RA Du Puy L., Beqqali A., Monshouwer-Kloots J., Haagsman H.P., Roelen B.A.,
RA Passier R.;
RT "CAZIP, a novel protein expressed in the developing heart and nervous
RT system.";
RL Dev. Dyn. 238:2903-2911(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-364 AND SER-365, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Heart;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Binds microtubules. Together with MAPRE1 may target the
CC microtubule depolymerase KIF2C to the plus-end of microtubules. May
CC regulate the dynamics of microtubules at their growing distal tip (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. Interacts with KIF2C and MAPRE1; the interaction is
CC direct and probably targets MTUS2 and KIF2C to microtubules (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Note=Associated with the
CC microtubule network at the growing distal tip (the plus-end) of
CC microtubules. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q3UHD3-1; Sequence=Displayed;
CC Name=3;
CC IsoId=Q3UHD3-3; Sequence=VSP_023545, VSP_023546;
CC Name=4;
CC IsoId=Q3UHD3-4; Sequence=VSP_023544, VSP_023548;
CC -!- TISSUE SPECIFICITY: Expressed in heart from early embryonic development
CC onwards. Later during embryonic development, expressed in brain and
CC nervous system, in limb buds and in the epithelium lining the bronchia
CC of the lung. Detected in adult brain, heart and eye, but not detected
CC in other adult tissues examined. {ECO:0000269|PubMed:19806667}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the MTUS1 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC41447.2; Type=Miscellaneous discrepancy; Note=Aberrant splicing.; Evidence={ECO:0000305};
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DR EMBL; AK019985; BAB31952.1; -; mRNA.
DR EMBL; AK147457; BAE27924.1; -; mRNA.
DR EMBL; BC055016; AAH55016.1; -; mRNA.
DR EMBL; BC150942; AAI50943.1; -; mRNA.
DR EMBL; AB093263; BAC41447.2; ALT_SEQ; Unassigned_RNA.
DR CCDS; CCDS19881.1; -. [Q3UHD3-1]
DR RefSeq; NP_084196.4; NM_029920.7. [Q3UHD3-1]
DR RefSeq; XP_006504953.1; XM_006504890.3.
DR AlphaFoldDB; Q3UHD3; -.
DR SMR; Q3UHD3; -.
DR BioGRID; 218742; 1.
DR IntAct; Q3UHD3; 1.
DR MINT; Q3UHD3; -.
DR STRING; 10090.ENSMUSP00000082694; -.
DR iPTMnet; Q3UHD3; -.
DR PhosphoSitePlus; Q3UHD3; -.
DR EPD; Q3UHD3; -.
DR MaxQB; Q3UHD3; -.
DR PaxDb; Q3UHD3; -.
DR PeptideAtlas; Q3UHD3; -.
DR PRIDE; Q3UHD3; -.
DR ProteomicsDB; 290120; -. [Q3UHD3-1]
DR ProteomicsDB; 290121; -. [Q3UHD3-3]
DR ProteomicsDB; 290122; -. [Q3UHD3-4]
DR Antibodypedia; 22727; 93 antibodies from 24 providers.
DR Ensembl; ENSMUST00000085554; ENSMUSP00000082690; ENSMUSG00000029651. [Q3UHD3-4]
DR Ensembl; ENSMUST00000085558; ENSMUSP00000082694; ENSMUSG00000029651. [Q3UHD3-1]
DR Ensembl; ENSMUST00000110515; ENSMUSP00000106144; ENSMUSG00000029651. [Q3UHD3-3]
DR GeneID; 77521; -.
DR KEGG; mmu:77521; -.
DR UCSC; uc009aon.2; mouse. [Q3UHD3-1]
DR UCSC; uc009aop.2; mouse. [Q3UHD3-3]
DR UCSC; uc009aoq.2; mouse. [Q3UHD3-4]
DR CTD; 23281; -.
DR MGI; MGI:1915388; Mtus2.
DR VEuPathDB; HostDB:ENSMUSG00000029651; -.
DR eggNOG; ENOG502QQFP; Eukaryota.
DR GeneTree; ENSGT00950000183026; -.
DR HOGENOM; CLU_005465_0_0_1; -.
DR InParanoid; Q3UHD3; -.
DR OMA; NTCQQKI; -.
DR OrthoDB; 250283at2759; -.
DR PhylomeDB; Q3UHD3; -.
DR TreeFam; TF333416; -.
DR BioGRID-ORCS; 77521; 1 hit in 58 CRISPR screens.
DR ChiTaRS; Mtus2; mouse.
DR PRO; PR:Q3UHD3; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q3UHD3; protein.
DR Bgee; ENSMUSG00000029651; Expressed in interventricular septum and 175 other tissues.
DR ExpressionAtlas; Q3UHD3; baseline and differential.
DR Genevisible; Q3UHD3; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005881; C:cytoplasmic microtubule; ISO:MGI.
DR GO; GO:0045171; C:intercellular bridge; ISO:MGI.
DR GO; GO:0015630; C:microtubule cytoskeleton; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR InterPro; IPR033339; MTUS2.
DR PANTHER; PTHR24200:SF14; PTHR24200:SF14; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton; Microtubule;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..1353
FT /note="Microtubule-associated tumor suppressor candidate 2
FT homolog"
FT /id="PRO_0000280112"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 81..101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 125..259
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 274..615
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 626..964
FT /note="Mediates interaction with MAPRE1"
FT /evidence="ECO:0000250"
FT REGION 786..1134
FT /note="Localization to the growing distal tip of
FT microtubules"
FT /evidence="ECO:0000250"
FT REGION 786..874
FT /note="Sufficient for interaction with KIF2C"
FT /evidence="ECO:0000250"
FT REGION 799..823
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 847..972
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1315..1353
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 976..1319
FT /evidence="ECO:0000255"
FT COMPBIAS 86..101
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 125..139
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 147..170
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 226..249
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 329..343
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 346..360
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 445..478
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 497..513
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 520..584
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 594..615
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 799..814
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 847..874
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 893..914
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 364
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 365
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..1201
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_023544"
FT VAR_SEQ 1..1005
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023545"
FT VAR_SEQ 1006..1023
FT /note="AIQGFDALAVSTKHFFGK -> MGHHCCKPYICLQCLDKT (in isoform
FT 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023546"
FT VAR_SEQ 1202..1210
FT /note="RKTTEEQLE -> MSLRTWARH (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_023548"
SQ SEQUENCE 1353 AA; 147356 MW; BFBFF33BA1A91B43 CRC64;
MSVPLAPKKS CFGQLRDHRE GAKNNNESIL RTGDTNANQI MLEVSSSCDE AKSRDLDDEL
GNSNLSRPQY HSHFQKEPLH LQGFGKGSQA GSTSQRESQA SLTVHRQLSE EHAVKRGALQ
APQCVQGPSL SSWRNAVGQA SPEASAKKDA EIPRHIPKDK LAKTLDNEEL KRASSCSAAA
GSVPPTDLQP VQLDTLGPQD HVPARGEGPQ RTPASHSPGK GFSPGEGTSE GNSVYLPKPS
TSEAKGSSPS DTKMEGPHGL DVYNERITHA ELTPSSASAS KENPGLRHPE VCLGQGTGKS
KVELKSVQPR NEEGLTSAQA QGPGCHEERS MSPVERQELL EKAYREATSQ GNSSHRQLGV
RRGSSLEEMT GVSAGVEGSQ QATPTLSAAP AGEAGTRLTG KMSAGVGRMA RETASGQTAP
DVGQAAPVRR DPTESVPSEV SGEERRLGSG NSGSTKLLAS GPSAGGSRTD TSGLLSPRGS
NLEARKGKEM VAENRNLLEN AAQTDNTPAG VDSAFSTPAP LLHPETTVNS SHHPTPPGSS
SQELGVFSGD TGSPSVASPP TDGGQVLNTS PKVPDRTTCS SGIPKPPTHP KDTPSSQEAR
EKLETEKMEE RAEAKPILMP KPKHVRPKII TYIRRNPQAL SQGDASLVPV GLPYAPPTCG
MPLPQEEKAA SRDLQPSANM YEKLKPDLQK PRVFPSGLMV SGIKPPAHHF SQMSEKFLQE
VADHPGKEEF CSPPYTHYEV PPTFYRSAML LKPQLGLGAM SRLPSTKSRI LIASQRSSAS
AIHPPGSLTT AASFYGSDPS DLKKASNSNA AKASLPKSGL RPPGYSRLPA AKLAAFGFVR
SSSISAVPSS QSLDSVQPEQ SRPVTRSTFG NEEQAPLKQA LPSKDTPKGA GRAAPASSSN
ATTPRRSLLP APKSTSTPAG AKKELQKDPE AKKPAVSSPK RTASAATKPH SPGYPKQRTS
APRNEFPPKP DLQAREAERQ LAQRLRDRCE WQARQLGLAR RELKKAIQGF DALAVSTKHF
FGKSERALAK EKELSIELAN IRDEVAFNTA KCEKLQKEKE TLERRFEEEL RRLGWQQQAE
VQELQERLQQ QFQAESARLQ AEHQDQLLRM RCQHQEQVED ITASHEAALL EMENNHTVAI
TILQDDHDHK VQELMSTHEF EKKELEENFE KLRLTLQDQV DTLTFQSQSL RDRARRFEEA
LRKTTEEQLE IALAPYQHLE EDMQSLKQVL EMKNQQIHLQ EKKIIELEKL VEKNIILEER
IQVLQQQNED LKARIDQNTV VTRQLSEENA NLQEYVEKET QEKKRLSRTN EELLWKLQTG
DPTSPIKLSP TSPVYRGSSS GPSSPARVST TPR
