MTW1_YEAST
ID MTW1_YEAST Reviewed; 289 AA.
AC P39731; D6VPI3;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Kinetochore-associated protein MTW1;
DE AltName: Full=Mis12-like protein;
GN Name=MTW1; Synonyms=DSN3, NSL2; OrderedLocusNames=YAL034W-A;
GN ORFNames=YAL034AW;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=10398680; DOI=10.1101/gad.13.13.1664;
RA Goshima G., Saitoh S., Yanagida M.;
RT "Proper metaphase spindle length is determined by centromere proteins Mis12
RT and Mis6 required for faithful chromosome segregation.";
RL Genes Dev. 13:1664-1677(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7731988; DOI=10.1073/pnas.92.9.3809;
RA Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N.,
RA Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J.,
RA Storms R.K.;
RT "The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995).
RN [3]
RP SEQUENCE REVISION.
RA Vo D.T.;
RL Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=12455957; DOI=10.1128/ec.1.2.229-240.2002;
RA Euskirchen G.M.;
RT "Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a new group of proteins important for
RT chromosome segregation in Saccharomyces cerevisiae.";
RL Eukaryot. Cell 1:229-240(2002).
RN [6]
RP FUNCTION, IDENTIFICATION IN THE MIND COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=14602074; DOI=10.1016/s1534-5807(03)00322-8;
RA Pinsky B.A., Tatsutani S.Y., Collins K.A., Biggins S.;
RT "An Mtw1 complex promotes kinetochore biorientation that is monitored by
RT the Ipl1/Aurora protein kinase.";
RL Dev. Cell 5:735-745(2003).
RN [7]
RP IDENTIFICATION IN THE MIND COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=14657030; DOI=10.1093/emboj/cdg636;
RA Scharfenberger M., Ortiz J., Grau N., Janke C., Schiebel E., Lechner J.;
RT "Nsl1p is essential for the establishment of bipolarity and the
RT localization of the Dam-Duo complex.";
RL EMBO J. 22:6584-6597(2003).
RN [8]
RP IDENTIFICATION IN THE MIND COMPLEX, AND FUNCTION OF THE MIND COMPLEX.
RX PubMed=14633972; DOI=10.1101/gad.1144403;
RA De Wulf P., McAinsh A.D., Sorger P.K.;
RT "Hierarchical assembly of the budding yeast kinetochore from multiple
RT subcomplexes.";
RL Genes Dev. 17:2902-2921(2003).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=14690591; DOI=10.1016/s1097-2765(03)00476-3;
RA Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M.,
RA Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E.,
RA Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N.;
RT "Assigning function to yeast proteins by integration of technologies.";
RL Mol. Cell 12:1353-1365(2003).
RN [10]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Acts as essential component of the kinetochore MIND complex,
CC which is required for the spindle checkpoint and kinetochore integrity.
CC MIND plays a role in establishing a bipolar spindle-kinetochore
CC interaction by joining kinetochore subunits contacting DNA to those
CC contacting microtubules. {ECO:0000269|PubMed:14602074,
CC ECO:0000269|PubMed:14633972}.
CC -!- SUBUNIT: Component of the MIND kinetochore complex, which is composed
CC of at least MTW1, NNF1, NSL1 and DSN1. {ECO:0000269|PubMed:14602074,
CC ECO:0000269|PubMed:14633972, ECO:0000269|PubMed:14657030}.
CC -!- INTERACTION:
CC P39731; P47149: NNF1; NbExp=6; IntAct=EBI-11606, EBI-12098;
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere, kinetochore
CC {ECO:0000269|PubMed:14602074, ECO:0000269|PubMed:14657030}. Cytoplasm,
CC cytoskeleton, spindle pole {ECO:0000269|PubMed:12455957}.
CC Note=Associated with the kinetochore. {ECO:0000269|PubMed:14602074,
CC ECO:0000269|PubMed:14657030}.
CC -!- MISCELLANEOUS: Present with 2610 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the mis12 family. {ECO:0000305}.
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DR EMBL; AB027473; BAA77792.1; -; Genomic_DNA.
DR EMBL; U12980; AAC04997.1; -; Genomic_DNA.
DR EMBL; BK006935; DAA06953.1; -; Genomic_DNA.
DR PIR; S70293; S70293.
DR RefSeq; NP_009367.1; NM_001178179.1.
DR AlphaFoldDB; P39731; -.
DR SMR; P39731; -.
DR BioGRID; 31731; 188.
DR ComplexPortal; CPX-1156; Central kinetochore CTF19 complex.
DR ComplexPortal; CPX-1186; Nuclear MIS12/MIND complex.
DR DIP; DIP-1464N; -.
DR IntAct; P39731; 17.
DR MINT; P39731; -.
DR STRING; 4932.YAL034W-A; -.
DR iPTMnet; P39731; -.
DR MaxQB; P39731; -.
DR PaxDb; P39731; -.
DR PRIDE; P39731; -.
DR EnsemblFungi; YAL034W-A_mRNA; YAL034W-A; YAL034W-A.
DR GeneID; 851197; -.
DR KEGG; sce:YAL034W-A; -.
DR SGD; S000000032; MTW1.
DR VEuPathDB; FungiDB:YAL034W-A; -.
DR eggNOG; ENOG502S72R; Eukaryota.
DR HOGENOM; CLU_046437_0_0_1; -.
DR InParanoid; P39731; -.
DR OMA; CTQAMET; -.
DR BioCyc; YEAST:G3O-28844-MON; -.
DR PRO; PR:P39731; -.
DR Proteomes; UP000002311; Chromosome I.
DR RNAct; P39731; protein.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000776; C:kinetochore; IPI:ComplexPortal.
DR GO; GO:0000818; C:nuclear MIS12/MIND complex; IDA:SGD.
DR GO; GO:0000922; C:spindle pole; IDA:SGD.
DR GO; GO:0008608; P:attachment of spindle microtubules to kinetochore; IC:ComplexPortal.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IDA:SGD.
DR GO; GO:0051382; P:kinetochore assembly; IBA:GO_Central.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IBA:GO_Central.
DR GO; GO:0034501; P:protein localization to kinetochore; IMP:SGD.
DR InterPro; IPR008685; Centromere_Mis12.
DR PANTHER; PTHR14527; PTHR14527; 1.
DR Pfam; PF05859; Mis12; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Cytoplasm;
KW Cytoskeleton; Kinetochore; Mitosis; Reference proteome.
FT CHAIN 1..289
FT /note="Kinetochore-associated protein MTW1"
FT /id="PRO_0000096641"
FT COILED 105..147
FT /evidence="ECO:0000255"
SQ SEQUENCE 289 AA; 33243 MW; D6F94C290106082B CRC64;
MSAPTMRSTS ILTEHLGYPP ISLVDDIINA VNEIMYKCTA AMEKYLLSKS KIGEEDYGEE
IKSGVAKLES LLENSVDKNF DKLELYVLRN VLRIPEEYLD ANVFRLENQK DLVIVDENEL
KKSEEKLREK VNDVELAFKK NEMLLKRVTK VKRLLFTIRG FKQKLNELLK CKDDVQLQKI
LESLKPIDDT MTLLTDSLRK LYVDSESTSS TEEVEALLQR LKTNGKQNNK DFRTRYIDIR
TNNVLRKLGL LGDKEDEKQS AKPDARTQAG DIVSIDIEEP QLDLLDDVL
