ID   MTX1_MACFA              Reviewed;         317 AA.
AC   Q4R3I0;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Metaxin-1;
DE   AltName: Full=Mitochondrial outer membrane import complex protein 1;
GN   Name=MTX1; ORFNames=QtsA-16827;
OS   Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9541;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   International consortium for macaque cDNA sequencing and analysis;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications.";
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in transport of proteins into the mitochondrion.
CC       Essential for embryonic development (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with MTX2/metaxin-2 (By similarity). Associates with
CC       the mitochondrial contact site and cristae organizing system (MICOS)
CC       complex, composed of at least MICOS10/MIC10, CHCHD3/MIC19,
CC       CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13
CC       (By similarity). This complex was also known under the names MINOS or
CC       MitOS complex (By similarity). The MICOS complex associates with
CC       mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together
CC       described as components of the mitochondrial outer membrane sorting
CC       assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner
CC       membrane protein TMEM11 and with HSPA9 (By similarity). The MICOS and
CC       SAM complexes together with DNAJC11 are part of a large protein complex
CC       spanning both membranes termed the mitochondrial intermembrane space
CC       bridging (MIB) complex (By similarity). Interacts with ARMC1 (By
CC       similarity). {ECO:0000250|UniProtKB:Q13505}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000250}.
CC   -!- PTM: Ubiquitinated by PRKN during mitophagy, leading to its degradation
CC       and enhancement of mitophagy. Deubiquitinated by USP30.
CC       {ECO:0000250|UniProtKB:Q13505}.
CC   -!- SIMILARITY: Belongs to the metaxin family. {ECO:0000305}.
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DR   EMBL; AB179286; BAE02337.1; -; mRNA.
DR   RefSeq; NP_001272082.1; NM_001285153.1.
DR   AlphaFoldDB; Q4R3I0; -.
DR   SMR; Q4R3I0; -.
DR   STRING; 9541.XP_005541625.1; -.
DR   GeneID; 101926624; -.
DR   CTD; 4580; -.
DR   eggNOG; KOG3028; Eukaryota.
DR   OrthoDB; 1472286at2759; -.
DR   Proteomes; UP000233100; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0001401; C:SAM complex; IEA:InterPro.
DR   GO; GO:0007005; P:mitochondrion organization; IEA:InterPro.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR   InterPro; IPR040079; Glutathione_S-Trfase.
DR   InterPro; IPR017410; Metaxin1/3.
DR   InterPro; IPR033468; Metaxin_GST.
DR   InterPro; IPR019564; Sam37/metaxin_N.
DR   Pfam; PF17171; GST_C_6; 1.
DR   Pfam; PF10568; Tom37; 1.
DR   PIRSF; PIRSF038150; Metaxin; 1.
DR   SFLD; SFLDS00019; Glutathione_Transferase_(cytos; 1.
DR   SUPFAM; SSF47616; SSF47616; 1.
PE   2: Evidence at transcript level;
KW   Isopeptide bond; Membrane; Mitochondrion; Mitochondrion outer membrane;
KW   Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport; Ubl conjugation.
FT   CHAIN           1..317
FT                   /note="Metaxin-1"
FT                   /id="PRO_0000320056"
FT   TRANSMEM        164..184
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CROSSLNK        38
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in ubiquitin)"
FT                   /evidence="ECO:0000250|UniProtKB:Q13505"
FT   CROSSLNK        41
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in ubiquitin)"
FT                   /evidence="ECO:0000250|UniProtKB:Q13505"
FT   CROSSLNK        78
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in ubiquitin)"
FT                   /evidence="ECO:0000250|UniProtKB:Q13505"
SQ   SEQUENCE   317 AA;  35678 MW;  0323DAC6D695AD5E CRC64;
     MAAPMELFCW SGGWGLPSVD LDSLAVLTYA RFTGAPLKVH KISNPWRSPS GTLPALRTSH
     GEVISVPHKI ITHLRKEKYN ADYDLSARQG ADTLAFMSLL EEKLLPVLVH TFWIDTKNYV
     EVTRKWYAEA MPFPLNFFLP GRMQRQYMER LELLSGEHMP EDEEELEKEL YREARECLTL
     LSQRLGSQKF FFGDAPASLD AFVFSYLALL LQAKLPSGKL QAHLRGLHNL CAYCTHILSL
     YFPWDGAEVP PPRQTPAGPE TEEEPYRRRN QILSVLAGLA AMVGYALLSG IVSIQRATPA
     RAPGTRALGM AEEDEEE