ID   MT_EISFE                Reviewed;          75 AA.
AC   P81695; O96822; Q2I6A2;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 53.
DE   RecName: Full=Cadmium-metallothionein;
DE            Short=MT;
DE   Flags: Precursor;
GN   Name=MT;
OS   Eisenia fetida (Red wiggler worm).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Annelida; Clitellata;
OC   Oligochaeta; Crassiclitellata; Lumbricina; Lumbricidae; Lumbricinae;
OC   Eisenia.
OX   NCBI_TaxID=6396;
RN   [1]
RP   PROTEIN SEQUENCE OF 1-41, NUCLEOTIDE SEQUENCE [MRNA] OF 2-75, SUBUNIT,
RP   CADMIUM-BINDING, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=10632728; DOI=10.1046/j.1432-1327.2000.01035.x;
RA   Gruber C., Stuerzenbaum S., Gehrig P., Sack R., Hunziker P., Berger B.,
RA   Dallinger R.;
RT   "Isolation and characterization of a self-sufficient one-domain protein.
RT   (Cd)-metallothionein from Eisenia foetida.";
RL   Eur. J. Biochem. 267:573-582(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 11-75, AND INDUCTION.
RC   TISSUE=Coelomocyte;
RX   PubMed=16683633; DOI=10.1021/es052299x;
RA   Brulle F., Mitta G., Cocquerelle C., Vieau D., Lemiere S., Lepretre A.,
RA   Vandenbulcke F.;
RT   "Cloning and real-time PCR testing of 14 potential biomarkers in Eisenia
RT   fetida following cadmium exposure.";
RL   Environ. Sci. Technol. 40:2844-2850(2006).
CC   -!- FUNCTION: Metallothioneins have a high content of cysteine residues
CC       that bind various heavy metals. Binds 4 cadmium ions per molecule.
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:10632728}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- TISSUE SPECIFICITY: Posterior alimentary canal.
CC   -!- INDUCTION: By cadmium. {ECO:0000269|PubMed:16683633}.
CC   -!- MASS SPECTROMETRY: Mass=4355.13; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:16683633};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ236886; CAA15423.1; -; mRNA.
DR   EMBL; DQ286714; ABC48927.1; -; mRNA.
DR   AlphaFoldDB; P81695; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR017980; Metalthion_4_echinoid/annelid.
DR   Pfam; PF05522; Metallothio_6; 1.
PE   1: Evidence at protein level;
KW   Cadmium; Cytoplasm; Direct protein sequencing; Metal-binding;
KW   Metal-thiolate cluster.
FT   CHAIN           1..41
FT                   /note="Cadmium-metallothionein"
FT                   /id="PRO_0000197428"
FT   PROPEP          42..75
FT                   /id="PRO_0000386445"
FT   CONFLICT        11
FT                   /note="A -> Q (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   75 AA;  7411 MW;  FDE2C30BA606494B CRC64;
     DTQCCGKSTC AREGSTCCCT NCRCLKSECL PGCKKLCCAD AEKGKCGNAG CKCGAACKCS
     AGSCAAGCKK GCCGD