MUB6_ARATH
ID MUB6_ARATH Reviewed; 119 AA.
AC Q8GWJ6; Q9LM54;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Membrane-anchored ubiquitin-fold protein 6;
DE Short=AtMUB6;
DE Short=Membrane-anchored ub-fold protein 6;
DE Flags: Precursor;
GN Name=MUB6; OrderedLocusNames=At1g22050; ORFNames=F2E2.12, F2E2_11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP IDENTIFICATION, NOMENCLATURE, ISOPRENYLATION AT CYS-116, METHYLATION AT
RP CYS-116, MUTAGENESIS OF CYS-116, TISSUE SPECIFICITY, INDUCTION, AND
RP SUBCELLULAR LOCATION.
RX PubMed=16831869; DOI=10.1074/jbc.m602283200;
RA Downes B.P., Saracco S.A., Lee S.S., Crowell D.N., Vierstra R.D.;
RT "MUBS: a family of ubiquitin-fold proteins that are plasma membrane-
RT anchored by prenylation.";
RL J. Biol. Chem. 281:27145-27157(2006).
CC -!- FUNCTION: May serve as docking site to facilitate the association of
CC other proteins to the plasma membrane.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:16831869};
CC Lipid-anchor {ECO:0000269|PubMed:16831869}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:16831869}.
CC -!- INDUCTION: Not induced by pathogens, cycloheximide and ozone treatment.
CC {ECO:0000269|PubMed:16831869}.
CC -!- MISCELLANEOUS: Heat stable and remains soluble at temperatures
CC exceeding 90 degrees Celsius.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF86549.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC069252; AAF86549.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30189.1; -; Genomic_DNA.
DR EMBL; AK118802; BAC43392.1; -; mRNA.
DR EMBL; BT005453; AAO63873.1; -; mRNA.
DR PIR; E86353; E86353.
DR RefSeq; NP_173624.1; NM_102055.3.
DR AlphaFoldDB; Q8GWJ6; -.
DR SMR; Q8GWJ6; -.
DR BioGRID; 24049; 6.
DR IntAct; Q8GWJ6; 1.
DR STRING; 3702.AT1G22050.1; -.
DR PaxDb; Q8GWJ6; -.
DR PRIDE; Q8GWJ6; -.
DR ProteomicsDB; 250742; -.
DR DNASU; 838810; -.
DR EnsemblPlants; AT1G22050.1; AT1G22050.1; AT1G22050.
DR GeneID; 838810; -.
DR Gramene; AT1G22050.1; AT1G22050.1; AT1G22050.
DR KEGG; ath:AT1G22050; -.
DR Araport; AT1G22050; -.
DR TAIR; locus:2030616; AT1G22050.
DR eggNOG; ENOG502S2V5; Eukaryota.
DR HOGENOM; CLU_136465_1_0_1; -.
DR InParanoid; Q8GWJ6; -.
DR OMA; NRCSCSI; -.
DR OrthoDB; 1555986at2759; -.
DR PhylomeDB; Q8GWJ6; -.
DR PRO; PR:Q8GWJ6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8GWJ6; baseline and differential.
DR Genevisible; Q8GWJ6; AT.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR017000; MUB.
DR InterPro; IPR000626; Ubiquitin-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR InterPro; IPR040015; UBL3-like.
DR InterPro; IPR039540; UBL3-like_ubiquitin_dom.
DR PANTHER; PTHR13169; PTHR13169; 1.
DR Pfam; PF13881; Rad60-SLD_2; 1.
DR PIRSF; PIRSF032572; MUB; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50053; UBIQUITIN_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Lipoprotein; Membrane; Methylation; Palmitate; Prenylation;
KW Reference proteome.
FT CHAIN 1..116
FT /note="Membrane-anchored ubiquitin-fold protein 6"
FT /id="PRO_0000248171"
FT PROPEP 117..119
FT /note="Removed in mature form"
FT /id="PRO_0000248172"
FT DOMAIN 8..76
FT /note="Ubiquitin-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
FT MOD_RES 116
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000269|PubMed:16831869"
FT LIPID 114
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT LIPID 116
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000269|PubMed:16831869"
FT MUTAGEN 116
FT /note="C->S: Loss of prenylation and membrane
FT localization."
FT /evidence="ECO:0000269|PubMed:16831869"
SQ SEQUENCE 119 AA; 13510 MW; 28F0773F22B53667 CRC64;
MAGEEDWIEL KFRLADGTDI GPSKYNQSMT VSSLKEKLIS QWPKDKENTP KTVNDMKLIN
AGKILENNRT LAESRLPVCE LPGMIITMHI VLRLPTLDKK SEKLQNDPPM KNRCVCTIL
