MUC20_MOUSE
ID MUC20_MOUSE Reviewed; 656 AA.
AC Q8BUE7; Q76I84; Q8R0X0;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Mucin-20;
DE Short=MUC-20;
DE Flags: Precursor;
GN Name=Muc20;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Kidney;
RX PubMed=14565953; DOI=10.1074/jbc.m304558200;
RA Higuchi T., Orita T., Nakanishi S., Katsuya K., Watanabe H., Yamasaki Y.,
RA Waga I., Nanayama T., Yamamoto Y., Munger W., Sun H.-W., Falk R.J.,
RA Jennette J.C., Alcorta D.A., Li H., Yamamoto T., Saito Y., Nakamura M.;
RT "Molecular cloning, genomic structure, and expression analysis of MUC20, a
RT novel mucin protein, up-regulated in injured kidney.";
RL J. Biol. Chem. 279:1968-1979(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INTERACTION WITH MET.
RX PubMed=15314156; DOI=10.1128/mcb.24.17.7456-7468.2004;
RA Higuchi T., Orita T., Katsuya K., Yamasaki Y., Akiyama K., Li H.,
RA Yamamoto T., Saito Y., Nakamura M.;
RT "MUC20 suppresses the hepatocyte growth factor-induced Grb2-Ras pathway by
RT binding to a multifunctional docking site of met.";
RL Mol. Cell. Biol. 24:7456-7468(2004).
CC -!- FUNCTION: May regulate MET signaling cascade. Seems to decrease
CC hepatocyte growth factor (HGF)-induced transient MAPK activation.
CC Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway.
CC Inhibits HGF-induced proliferation of MMP1 and MMP9 expression (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with MET; oligomerization increases affinity for
CC MET. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Apical cell membrane
CC {ECO:0000250}. Basolateral cell membrane {ECO:0000250}. Cell
CC projection, microvillus membrane {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Highly expressed in kidney. Up-regulated in renal
CC tissues during renal injury. {ECO:0000269|PubMed:14565953}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH26367.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Mucin database;
CC URL="http://www.medkem.gu.se/mucinbiology/databases/";
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DR EMBL; AB098732; BAD06719.1; -; mRNA.
DR EMBL; AK085640; BAC39492.1; -; mRNA.
DR EMBL; AK165477; BAE38212.1; -; mRNA.
DR EMBL; BC026367; AAH26367.1; ALT_INIT; mRNA.
DR CCDS; CCDS49830.1; -.
DR RefSeq; NP_001139346.1; NM_001145874.1.
DR RefSeq; NP_666183.2; NM_146071.2.
DR AlphaFoldDB; Q8BUE7; -.
DR BioGRID; 230247; 1.
DR STRING; 10090.ENSMUSP00000110769; -.
DR GlyGen; Q8BUE7; 2 sites.
DR iPTMnet; Q8BUE7; -.
DR PhosphoSitePlus; Q8BUE7; -.
DR PaxDb; Q8BUE7; -.
DR PRIDE; Q8BUE7; -.
DR ProteomicsDB; 287524; -.
DR Antibodypedia; 35139; 211 antibodies from 24 providers.
DR DNASU; 224116; -.
DR Ensembl; ENSMUST00000041123; ENSMUSP00000041221; ENSMUSG00000035638.
DR GeneID; 224116; -.
DR KEGG; mmu:224116; -.
DR UCSC; uc007yzi.2; mouse.
DR CTD; 200958; -.
DR MGI; MGI:2385039; Muc20.
DR VEuPathDB; HostDB:ENSMUSG00000035638; -.
DR eggNOG; ENOG502SVUK; Eukaryota.
DR GeneTree; ENSGT00730000111453; -.
DR HOGENOM; CLU_026298_0_0_1; -.
DR InParanoid; Q8BUE7; -.
DR OrthoDB; 878577at2759; -.
DR PhylomeDB; Q8BUE7; -.
DR Reactome; R-MMU-8851805; MET activates RAS signaling.
DR Reactome; R-MMU-913709; O-linked glycosylation of mucins.
DR Reactome; R-MMU-977068; Termination of O-glycan biosynthesis.
DR BioGRID-ORCS; 224116; 0 hits in 72 CRISPR screens.
DR PRO; PR:Q8BUE7; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q8BUE7; protein.
DR Bgee; ENSMUSG00000035638; Expressed in lumbar subsegment of spinal cord and 50 other tissues.
DR ExpressionAtlas; Q8BUE7; baseline and differential.
DR Genevisible; Q8BUE7; MM.
DR GO; GO:0016324; C:apical plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009925; C:basal plasma membrane; ISO:MGI.
DR GO; GO:0016323; C:basolateral plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0031528; C:microvillus membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005741; C:mitochondrial outer membrane; ISS:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0048012; P:hepatocyte growth factor receptor signaling pathway; IDA:MGI.
DR GO; GO:0043065; P:positive regulation of apoptotic process; ISS:UniProtKB.
DR GO; GO:0043410; P:positive regulation of MAPK cascade; IPI:MGI.
DR InterPro; IPR034551; MUC20.
DR PANTHER; PTHR37358; PTHR37358; 2.
PE 1: Evidence at protein level;
KW Cell membrane; Cell projection; Glycoprotein; Membrane; Reference proteome;
KW Repeat; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..656
FT /note="Mucin-20"
FT /id="PRO_0000317470"
FT REPEAT 180..188
FT /note="1"
FT REPEAT 189..197
FT /note="2"
FT REPEAT 198..206
FT /note="3"
FT REPEAT 210..218
FT /note="4"
FT REPEAT 219..227
FT /note="5"
FT REGION 85..125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 159..232
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 180..227
FT /note="Approximate repeats"
FT REGION 329..348
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 399..603
FT /note="Involved in oligomerization"
FT /evidence="ECO:0000250"
FT REGION 560..592
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 604..656
FT /note="Interaction with MET"
FT /evidence="ECO:0000250"
FT CARBOHYD 366
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 570
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 99
FT /note="T -> A (in Ref. 1; BAD06719)"
FT /evidence="ECO:0000305"
FT CONFLICT 199
FT /note="T -> I (in Ref. 1; BAD06719)"
FT /evidence="ECO:0000305"
FT CONFLICT 215
FT /note="P -> L (in Ref. 1; BAD06719)"
FT /evidence="ECO:0000305"
FT CONFLICT 219
FT /note="T -> A (in Ref. 1; BAD06719)"
FT /evidence="ECO:0000305"
FT CONFLICT 262
FT /note="T -> K (in Ref. 1; BAD06719)"
FT /evidence="ECO:0000305"
FT CONFLICT 273
FT /note="A -> T (in Ref. 1; BAD06719)"
FT /evidence="ECO:0000305"
FT CONFLICT 293
FT /note="S -> P (in Ref. 1; BAD06719)"
FT /evidence="ECO:0000305"
FT CONFLICT 297
FT /note="V -> I (in Ref. 1; BAD06719)"
FT /evidence="ECO:0000305"
FT CONFLICT 329
FT /note="Y -> H (in Ref. 1; BAD06719)"
FT /evidence="ECO:0000305"
FT CONFLICT 374
FT /note="N -> T (in Ref. 1; BAD06719)"
FT /evidence="ECO:0000305"
FT CONFLICT 391
FT /note="A -> V (in Ref. 1; BAD06719)"
FT /evidence="ECO:0000305"
FT CONFLICT 509
FT /note="A -> T (in Ref. 1; BAD06719)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 656 AA; 68720 MW; 5A0BECDE6F3297DA CRC64;
MGSVWGLAVP LLVFCWKVGV SVSSPGLDIS RSVPLVTTNN MEVSTFTQRD RPSSERAFQT
TNLIQYVPLD TQTLSTESAS NALSATSISS EVNSRDTQTT SFVTKTRKTH TTTPAASSLE
AQTTSPNLST LNIQITSPIA SSLDAQTIFP VSLSLSTQTT SPAPSFLDTQ TTSPEPSSLT
TSPAPSSLIT SPTPSSLTTS PAPSFLDTQT TSPAPSSLTT SPAPSSLDTQ TISPIELTLK
TQTISTVTET RTVSIRIPSD LTVMHTIPTE TLAPSNSPRT GMSTVQTGTV WDSIEAVFDT
LCTDDSSEEA RKITVDLLTL AHTSTEVEYL SSESSSSSDS SAGVLSSSRV LGPDSATPAK
GLVAFNITHI KLSNCITEIE TTITISGAPG ASLSPTEATA ALFTSEILTL PPPTEAKPIF
PETTSLSGIL STAGTPALAT TLEGTVSTSA ITESETAVAQ TLTSVGTSVT VRRNPLENTS
TLSIETQSHT EVLGTITVPM VAGSTMGEAA SFVSFTALDS SSLSVVVTTE SSATSETLTT
GNTTNSSFLT ESHPPFSIYS TTASTSKNPN ITLTKTTASP KPPTHPTTSA STAWIRKTTK
HDPGEDGGFL LVRLTVASPK DLTEHNAREK LMNQLRRELH ARMPLVHMSF LSIRRG
