MUCA_PSEAE
ID MUCA_PSEAE Reviewed; 194 AA.
AC P38107;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Sigma factor AlgU negative regulatory protein;
GN Name=mucA; OrderedLocusNames=PA0763;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=8412698; DOI=10.1111/j.1365-2958.1993.tb01711.x;
RA Martin D.W., Schurr M.J., Mudd M.H., Deretic V.;
RT "Differentiation of Pseudomonas aeruginosa into the alginate-producing
RT form: inactivation of mucB causes conversion to mucoidy.";
RL Mol. Microbiol. 9:497-506(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAO381, and PAO568;
RX PubMed=8378309; DOI=10.1073/pnas.90.18.8377;
RA Martin D.W., Schurr M.J., Mudd M.H., Govan J.R.W., Holloway B.W.,
RA Deretic V.;
RT "Mechanism of conversion to mucoidy in Pseudomonas aeruginosa infecting
RT cystic fibrosis patients.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:8377-8381(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Negative regulator of the sigma factor AlgU. Plays a role in
CC the differentiation of P.aeruginosa into the alginate-producing form.
CC Inactivation of mucA causes a switch from the non-mucoid to mucoid
CC state resulting in constitutive expression of alginate biosynthetic
CC genes.
CC -!- INTERACTION:
CC P38107; Q9HVX1: algW; NbExp=3; IntAct=EBI-6406981, EBI-6407097;
CC P38107; P38108: mucB; NbExp=4; IntAct=EBI-6406981, EBI-6406975;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the RseA family. {ECO:0000305}.
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DR EMBL; L14760; AAA87629.1; -; Genomic_DNA.
DR EMBL; U49151; AAC43715.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG04152.1; -; Genomic_DNA.
DR PIR; S36207; S36207.
DR RefSeq; NP_249454.1; NC_002516.2.
DR RefSeq; WP_003101958.1; NZ_QZGE01000007.1.
DR PDB; 6IN7; X-ray; 1.96 A; A=1-80.
DR PDB; 6IN9; X-ray; 1.80 A; C/D=106-194.
DR PDB; 6JAU; X-ray; 1.91 A; B=106-194.
DR PDBsum; 6IN7; -.
DR PDBsum; 6IN9; -.
DR PDBsum; 6JAU; -.
DR AlphaFoldDB; P38107; -.
DR SMR; P38107; -.
DR IntAct; P38107; 3.
DR STRING; 287.DR97_1220; -.
DR PaxDb; P38107; -.
DR PRIDE; P38107; -.
DR EnsemblBacteria; AAG04152; AAG04152; PA0763.
DR GeneID; 879357; -.
DR KEGG; pae:PA0763; -.
DR PATRIC; fig|208964.12.peg.793; -.
DR PseudoCAP; PA0763; -.
DR HOGENOM; CLU_105872_1_0_6; -.
DR InParanoid; P38107; -.
DR OMA; PEWNIAE; -.
DR PhylomeDB; P38107; -.
DR BioCyc; PAER208964:G1FZ6-776-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016989; F:sigma factor antagonist activity; IMP:PseudoCAP.
DR GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0032885; P:regulation of polysaccharide biosynthetic process; IMP:PseudoCAP.
DR CDD; cd16328; RseA_N; 1.
DR Gene3D; 1.10.10.880; -; 1.
DR InterPro; IPR005573; Anti-sigma_E_RseA_C.
DR InterPro; IPR005572; Anti-sigma_E_RseA_N.
DR InterPro; IPR036147; Anti-sigma_E_RseA_N_sf.
DR Pfam; PF03873; RseA_C; 1.
DR Pfam; PF03872; RseA_N; 1.
DR SUPFAM; SSF89069; SSF89069; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alginate biosynthesis; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..194
FT /note="Sigma factor AlgU negative regulatory protein"
FT /id="PRO_0000096642"
FT TRANSMEM 89..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT HELIX 3..14
FT /evidence="ECO:0007829|PDB:6IN7"
FT HELIX 20..32
FT /evidence="ECO:0007829|PDB:6IN7"
FT HELIX 34..50
FT /evidence="ECO:0007829|PDB:6IN7"
FT HELIX 62..70
FT /evidence="ECO:0007829|PDB:6IN7"
FT HELIX 153..160
FT /evidence="ECO:0007829|PDB:6IN9"
FT HELIX 163..177
FT /evidence="ECO:0007829|PDB:6IN9"
FT HELIX 183..189
FT /evidence="ECO:0007829|PDB:6IN9"
SQ SEQUENCE 194 AA; 20999 MW; 421755F4F113769B CRC64;
MSREALQETL SAVMDNEADE LELRRVLAAC GEDAELRSTW SRYQLARSVM HREPTLPKLD
IAAAVSAALA DEAAPPKAEK GPWRMVGRLA VAASVTLAVL AGVRLYNQND ALPQMAQQGT
TPQIALPQVK GPAVLAGYSE EQGAPQVITN SSSSDTRWHE QRLPIYLRQH VQQSAVSGTE
SALPYARAAS LENR
