MUCB_PSEAE
ID MUCB_PSEAE Reviewed; 316 AA.
AC P38108;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Sigma factor AlgU regulatory protein MucB;
DE Flags: Precursor;
GN Name=mucB; Synonyms=algN; OrderedLocusNames=PA0764;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=8412698; DOI=10.1111/j.1365-2958.1993.tb01711.x;
RA Martin D.W., Schurr M.J., Mudd M.H., Deretic V.;
RT "Differentiation of Pseudomonas aeruginosa into the alginate-producing
RT form: inactivation of mucB causes conversion to mucoidy.";
RL Mol. Microbiol. 9:497-506(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAO381, and PAO568;
RX PubMed=8378309; DOI=10.1073/pnas.90.18.8377;
RA Martin D.W., Schurr M.J., Mudd M.H., Govan J.R.W., Holloway B.W.,
RA Deretic V.;
RT "Mechanism of conversion to mucoidy in Pseudomonas aeruginosa infecting
RT cystic fibrosis patients.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:8377-8381(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8444793; DOI=10.1128/jb.175.5.1303-1308.1993;
RA Goldberg J.B., Gorman W.L., Flynn J.L., Ohman D.E.;
RT "A mutation in algN permits trans activation of alginate production by algT
RT in Pseudomonas species.";
RL J. Bacteriol. 175:1303-1308(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Negative regulator of the sigma factor AlgU. Plays a role in
CC the differentiation of P.aeruginosa into the alginate-producing form.
CC Inactivation of mucB causes conversion to mucoidy.
CC -!- INTERACTION:
CC P38108; P38107: mucA; NbExp=4; IntAct=EBI-6406975, EBI-6406981;
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the RseB family. {ECO:0000305}.
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DR EMBL; L09122; AAA25702.1; -; Genomic_DNA.
DR EMBL; L14760; AAA87630.1; -; Genomic_DNA.
DR EMBL; L14761; AAA87633.1; -; Genomic_DNA.
DR EMBL; U49151; AAC43716.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG04153.1; -; Genomic_DNA.
DR PIR; A47064; A47064.
DR RefSeq; NP_249455.1; NC_002516.2.
DR RefSeq; WP_003101960.1; NZ_QZGE01000007.1.
DR PDB; 6IN8; X-ray; 2.20 A; A=22-316.
DR PDB; 6IN9; X-ray; 1.80 A; A/B=22-316.
DR PDB; 6JAU; X-ray; 1.91 A; A=22-316.
DR PDBsum; 6IN8; -.
DR PDBsum; 6IN9; -.
DR PDBsum; 6JAU; -.
DR AlphaFoldDB; P38108; -.
DR SMR; P38108; -.
DR IntAct; P38108; 1.
DR STRING; 287.DR97_1219; -.
DR PaxDb; P38108; -.
DR PRIDE; P38108; -.
DR EnsemblBacteria; AAG04153; AAG04153; PA0764.
DR GeneID; 882064; -.
DR KEGG; pae:PA0764; -.
DR PATRIC; fig|208964.12.peg.794; -.
DR PseudoCAP; PA0764; -.
DR HOGENOM; CLU_054710_0_1_6; -.
DR InParanoid; P38108; -.
DR OMA; DDFRYQY; -.
DR PhylomeDB; P38108; -.
DR BioCyc; PAER208964:G1FZ6-777-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IBA:GO_Central.
DR GO; GO:0045152; F:antisigma factor binding; IBA:GO_Central.
DR GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0032885; P:regulation of polysaccharide biosynthetic process; IMP:PseudoCAP.
DR CDD; cd16327; RseB; 1.
DR Gene3D; 3.30.200.100; -; 1.
DR InterPro; IPR033436; MucB/RseB_C.
DR InterPro; IPR038484; MucB/RseB_C_sf.
DR InterPro; IPR033434; MucB/RseB_N.
DR InterPro; IPR005588; MucB_RseB.
DR PANTHER; PTHR38782; PTHR38782; 1.
DR Pfam; PF03888; MucB_RseB; 1.
DR Pfam; PF17188; MucB_RseB_C; 1.
DR PIRSF; PIRSF005427; RseB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alginate biosynthesis; Periplasm; Reference proteome; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..316
FT /note="Sigma factor AlgU regulatory protein MucB"
FT /id="PRO_0000021786"
FT HELIX 24..37
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 40..48
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 51..61
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 67..73
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 75..77
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 80..84
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 87..92
FT /evidence="ECO:0007829|PDB:6IN9"
FT HELIX 93..95
FT /evidence="ECO:0007829|PDB:6IN9"
FT TURN 96..98
FT /evidence="ECO:0007829|PDB:6IN9"
FT HELIX 101..104
FT /evidence="ECO:0007829|PDB:6JAU"
FT HELIX 115..117
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 119..129
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 132..143
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 148..154
FT /evidence="ECO:0007829|PDB:6IN9"
FT TURN 155..157
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 160..166
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 172..185
FT /evidence="ECO:0007829|PDB:6IN9"
FT HELIX 189..192
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 198..200
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 213..217
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 224..232
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 234..237
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 239..246
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 251..258
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 267..271
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 274..281
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 288..296
FT /evidence="ECO:0007829|PDB:6IN9"
FT HELIX 298..305
FT /evidence="ECO:0007829|PDB:6IN9"
FT STRAND 308..310
FT /evidence="ECO:0007829|PDB:6IN9"
SQ SEQUENCE 316 AA; 34572 MW; DC13C21A7BBB9C2C CRC64;
MRTTSLLLLL GSLMAVPATQ AADASDWLNR LAEADRQNSF QGTFVYERNG SFSTHEIWHR
VESDGAVRER LLQLDGARQE VVRVDGRTQC ISGGLADQLA DAQLWPVRKF DPSQLASWYD
LRLVGESRVA GRPAVVLAVT PRDQHRYGFE LHLDRDTGLP LKSLLLNEKG QLLERFQFTQ
LNTGAAPAED QLQAGAECQV VGPAKADGEK TVAWRSEWLP PGFTLTRSFM RRSPVTPDPV
ACLTYGDGLA RFSVFIEPLH GAMVGDARSQ LGPTVVVSKR LQTDDGGQMV TVVGEVPLGT
AERVALSIRP EAAAQK
