MUG20_SCHPO
ID MUG20_SCHPO Reviewed; 151 AA.
AC Q9HGN4;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2012, sequence version 2.
DT 25-MAY-2022, entry version 75.
DE RecName: Full=Linear element protein Mug20 {ECO:0000312|PomBase:SPBC36B7.06c};
DE AltName: Full=Meiotically up-regulated gene 20 protein;
GN Name=mug20 {ECO:0000312|PomBase:SPBC36B7.06c};
GN ORFNames=SPBC36B7.06c {ECO:0000312|PomBase:SPBC36B7.06c};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [3]
RP DEVELOPMENTAL STAGE.
RX PubMed=16303567; DOI=10.1016/j.cub.2005.10.038;
RA Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L.,
RA Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P.,
RA Smith G.R., Moreno S.;
RT "A large-scale screen in S. pombe identifies seven novel genes required for
RT critical meiotic events.";
RL Curr. Biol. 15:2056-2062(2005).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND INTERACTION WITH REC10.
RX PubMed=19756689; DOI=10.1007/s00412-009-0241-5;
RA Spirek M., Estreicher A., Csaszar E., Wells J., McFarlane R.J., Watts F.Z.,
RA Loidl J.;
RT "SUMOylation is required for normal development of linear elements and
RT wild-type meiotic recombination in Schizosaccharomyces pombe.";
RL Chromosoma 119:59-72(2010).
RN [6]
RP FUNCTION, IDENTIFICATION IN THE LINEAR ELEMENT COMPLEX, SUBCELLULAR
RP LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=23395004; DOI=10.1016/j.molcel.2013.01.008;
RA Fowler K.R., Gutierrez-Velasco S., Martin-Castellanos C., Smith G.R.;
RT "Protein determinants of meiotic DNA break hot spots.";
RL Mol. Cell 49:983-996(2013).
RN [7]
RP MUTAGENESIS OF LEU-52 AND VAL-78.
RX PubMed=28469148; DOI=10.1038/s41598-017-00742-3;
RA Ma L., Fowler K.R., Martin-Castellanos C., Smith G.R.;
RT "Functional organization of protein determinants of meiotic DNA break
RT hotspots.";
RL Sci. Rep. 7:1393-1393(2017).
RN [8]
RP SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=33825974; DOI=10.1007/s00412-021-00757-w;
RA Ding D.Q., Matsuda A., Okamasa K., Hiraoka Y.;
RT "Linear elements are stable structures along the chromosome axis in fission
RT yeast meiosis.";
RL Chromosoma 130:s00412.021.00757-s00412.021.00757(2021).
CC -!- FUNCTION: During meiotic DNA recombination, binds to and may help
CC activate DNA double-strand break (DSB) hotspot sites.
CC {ECO:0000269|PubMed:23395004}.
CC -!- SUBUNIT: Component of linear elements (LinEs), which are similar to
CC synaptonemal complexes, at least composed of rec27, rec25, rec10 and
CC mug20 (PubMed:23395004). Interacts with rec10 (PubMed:19756689).
CC {ECO:0000269|PubMed:19756689, ECO:0000269|PubMed:23395004}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC {ECO:0000269|PubMed:16823372, ECO:0000269|PubMed:23395004,
CC ECO:0000269|PubMed:33825974}. Chromosome {ECO:0000269|PubMed:23395004}.
CC Note=Localizes to chromosomal double-strand break (DSB) hotspot sites
CC during the meiotic cell cycle. {ECO:0000269|PubMed:23395004}.
CC -!- DEVELOPMENTAL STAGE: Up-regulated during meiosis (PubMed:16303567).
CC Present from pre-meiotic DNA replication and disappears following
CC meiotic prophase I (PubMed:23395004, PubMed:33825974).
CC {ECO:0000269|PubMed:16303567, ECO:0000269|PubMed:23395004,
CC ECO:0000269|PubMed:33825974}.
CC -!- DISRUPTION PHENOTYPE: Abnormal linear element formation
CC (PubMed:33825974, PubMed:23395004). Decreases meiotic gene conversion
CC at ade6 (PubMed:23395004). Decreases crossing-over between ade6 and
CC arg1, and lys4 and his4 (PubMed:23395004). Abnormal sporulation
CC (PubMed:33825974). {ECO:0000269|PubMed:23395004,
CC ECO:0000269|PubMed:33825974}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CU329671; CAC05727.2; -; Genomic_DNA.
DR RefSeq; NP_595988.2; NM_001021895.2.
DR AlphaFoldDB; Q9HGN4; -.
DR SMR; Q9HGN4; -.
DR BioGRID; 277506; 53.
DR STRING; 4896.SPBC36B7.06c.1; -.
DR PaxDb; Q9HGN4; -.
DR EnsemblFungi; SPBC36B7.06c.1; SPBC36B7.06c.1:pep; SPBC36B7.06c.
DR GeneID; 2540990; -.
DR KEGG; spo:SPBC36B7.06c; -.
DR PomBase; SPBC36B7.06c; mug20.
DR VEuPathDB; FungiDB:SPBC36B7.06c; -.
DR HOGENOM; CLU_1732541_0_0_1; -.
DR OMA; HDRVLQS; -.
DR PRO; PR:Q9HGN4; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0000785; C:chromatin; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0030998; C:linear element; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:1903343; P:positive regulation of meiotic DNA double-strand break formation; EXP:PomBase.
DR GO; GO:0062209; P:spatial regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination; EXP:PomBase.
PE 1: Evidence at protein level;
KW Chromosome; Coiled coil; Cytoplasm; Meiosis; Nucleus; Reference proteome.
FT CHAIN 1..151
FT /note="Linear element protein Mug20"
FT /id="PRO_0000278495"
FT COILED 56..140
FT /evidence="ECO:0000255"
FT MUTAGEN 52
FT /note="L->P: Decreases meiotic gene conversion at ade6,
FT crossing-over between ade6 and arg1, and abolishes double-
FT strand break (DSB) formation at a DSB hotspot."
FT /evidence="ECO:0000269|PubMed:28469148"
FT MUTAGEN 78
FT /note="V->E: Decreases meiotic gene conversion at ade6,
FT crossing-over between ade6 and arg1, and double-strand
FT break (DSB) formation at a DSB hotspot."
FT /evidence="ECO:0000269|PubMed:28469148"
SQ SEQUENCE 151 AA; 17239 MW; CB2B62175FD5CE78 CRC64;
MSEIITSLQT ELSNRHANMV SEAVLAFSEH HDSALHSYFL NARVKDVILE SLRTTEEKLR
ALDKLQLDAS KNLSNLLVEY NSINAELESV NSKISSISSQ EYALEEFQAL SQNFEKNLVQ
HRRNSQNKLK CAMEKLKMIE TSTHKAILDN F
