MUG61_SCHPO
ID MUG61_SCHPO Reviewed; 844 AA.
AC O13712; Q9USD6;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Meiotically up-regulated gene 61 protein;
GN Name=mug61; ORFNames=SPAC14C4.05c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 637-835, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=ATCC 38364 / 968;
RX PubMed=10759889; DOI=10.1046/j.1365-2443.2000.00317.x;
RA Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T.,
RA Hiraoka Y.;
RT "Large-scale screening of intracellular protein localization in living
RT fission yeast cells by the use of a GFP-fusion genomic DNA library.";
RL Genes Cells 5:169-190(2000).
RN [3]
RP INTERACTION WITH SAD1, AND SUBCELLULAR LOCATION.
RX PubMed=14655046; DOI=10.1007/s00438-003-0938-8;
RA Miki F., Kurabayashi A., Tange Y., Okazaki K., Shimanuki M., Niwa O.;
RT "Two-hybrid search for proteins that interact with Sad1 and Kms1, two
RT membrane-bound components of the spindle pole body in fission yeast.";
RL Mol. Genet. Genomics 270:449-461(2004).
RN [4]
RP FUNCTION IN MEIOSIS.
RX PubMed=16303567; DOI=10.1016/j.cub.2005.10.038;
RA Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L.,
RA Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P.,
RA Smith G.R., Moreno S.;
RT "A large-scale screen in S. pombe identifies seven novel genes required for
RT critical meiotic events.";
RL Curr. Biol. 15:2056-2062(2005).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Required for correct meiotic chromosome segregation.
CC {ECO:0000269|PubMed:16303567}.
CC -!- SUBUNIT: Interacts with sad1. {ECO:0000269|PubMed:14655046}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC membrane protein. Nucleus membrane; Multi-pass membrane protein.
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DR EMBL; CU329670; CAB11198.1; -; Genomic_DNA.
DR EMBL; AB027838; BAA87142.1; -; Genomic_DNA.
DR PIR; T37690; T37690.
DR RefSeq; NP_594910.1; NM_001020342.2.
DR AlphaFoldDB; O13712; -.
DR SMR; O13712; -.
DR BioGRID; 278082; 146.
DR IntAct; O13712; 18.
DR STRING; 4896.SPAC14C4.05c.1; -.
DR iPTMnet; O13712; -.
DR MaxQB; O13712; -.
DR PaxDb; O13712; -.
DR PRIDE; O13712; -.
DR EnsemblFungi; SPAC14C4.05c.1; SPAC14C4.05c.1:pep; SPAC14C4.05c.
DR GeneID; 2541585; -.
DR KEGG; spo:SPAC14C4.05c; -.
DR PomBase; SPAC14C4.05c; -.
DR VEuPathDB; FungiDB:SPAC14C4.05c; -.
DR HOGENOM; CLU_337438_0_0_1; -.
DR InParanoid; O13712; -.
DR OMA; FCEIPEE; -.
DR PRO; PR:O13712; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0034506; C:chromosome, centromeric core domain; IDA:PomBase.
DR GO; GO:0099115; C:chromosome, subtelomeric region; IDA:PomBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000792; C:heterochromatin; IDA:PomBase.
DR GO; GO:0005639; C:integral component of nuclear inner membrane; IDA:PomBase.
DR GO; GO:0005635; C:nuclear envelope; IDA:PomBase.
DR GO; GO:0031965; C:nuclear membrane; IDA:PomBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003682; F:chromatin binding; IEA:InterPro.
DR GO; GO:0062239; F:heterochromatin-nuclear membrane anchor activity; EXP:PomBase.
DR GO; GO:0140698; P:attachment of telomeric heterochromatin to nuclear envelope; IMP:PomBase.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0071763; P:nuclear membrane organization; IMP:PomBase.
DR InterPro; IPR025856; HeH/LEM_domain.
DR InterPro; IPR044780; Heh2/Src1.
DR InterPro; IPR018996; MSC.
DR PANTHER; PTHR47808; PTHR47808; 1.
DR Pfam; PF12949; HeH; 1.
DR Pfam; PF09402; MSC; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Chromosome partition; Endoplasmic reticulum; Meiosis;
KW Membrane; Nucleotide-binding; Nucleus; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..844
FT /note="Meiotically up-regulated gene 61 protein"
FT /id="PRO_0000116682"
FT TRANSMEM 459..479
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 705..725
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 333..354
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 384..415
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 394..411
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 605..612
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 844 AA; 94908 MW; D31C398350014766 CRC64;
MEVPSYFDPD YDPSSLRVVD LRNILTEYQI YYPSTAKKAQ LITLFSKLRR AKNGLISMTE
LQQKNVPPSS RSPRRRVAGV TNNVTARISS KRKINMVDEA NDTEISKTSQ FEDNVMGMLQ
DENVQVLNTN TITISEESEF HASKIAKIDS RNEEITHIPF ETQTELNAAV VNLDNSMESS
FSIVQNLTNK DSSVDTATYD FSAEVGNIVT PASKFLDYDQ SYLVNASVSG DPTPVKVLNT
TSPKSENPLN QSSFLSFLGE NLKPKFTSRS SSVYASPIKS SLNSLECNPS NLLSVRKNFQ
QSSDSYLKSN KSFDQLNNLV GLSTGNSENF TPENNSFSWT HPKKNSSSPL PQSQSSSIFV
EHLNQLYEAN ASIHRPVNPA FSTNFGLEAS NTSTPEKKKF DSQKPDDDSV NEISSDLGLS
TTGIDRVEEN ISLTKDRQPK RPYFSLGSFI SLIFSFTKVV NSLWLVLLVV PLLGFVGFWH
QEVQRVGFCG VPAEPYPSSL YYLQPGVLRS SIESAYSFAH SLGIEASCQP CPENAECGFN
RQLFCKEGLK ASFPLLADFG LKPYPRCIPN TVKVNKVEEM VQAFMSIIGK WYYKAPKEFA
TFESAKNLNG KSFVDNFKDR YYMYKQDIDN VVGLKDFKVY LKTTLNRLYN SKLTRKVLYY
LFSPLFTLEL WKLRVRGALS KFPTNCLRSV YSHTVSLMKY LTSAVISCWR IYLLIGILAA
ITGTVVWRIR VYAKKHVVKH GVSVCVSHCI AKLQKTKLKS LTDFSVNPRV EVVQLRSDCF
VSGVADDKGL FELVHLPLSI QLEIWEKVVS VLEGMVSVKV WDSERLAKNR AWEWIGVFSD
DIAL
