ID   MUKB_AGGAC              Reviewed;        1496 AA.
AC   Q93IE8;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Chromosome partition protein MukB {ECO:0000255|HAMAP-Rule:MF_01800};
DE   AltName: Full=Structural maintenance of chromosome-related protein {ECO:0000255|HAMAP-Rule:MF_01800};
GN   Name=mukB {ECO:0000255|HAMAP-Rule:MF_01800};
OS   Aggregatibacter actinomycetemcomitans (Actinobacillus
OS   actinomycetemcomitans) (Haemophilus actinomycetemcomitans).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Aggregatibacter.
OX   NCBI_TaxID=714;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=14660695; DOI=10.1093/molbev/msh023;
RA   Cobbe N., Heck M.M.S.;
RT   "The evolution of SMC proteins: phylogenetic analysis and structural
RT   implications.";
RL   Mol. Biol. Evol. 21:332-347(2004).
CC   -!- FUNCTION: Plays a central role in chromosome condensation, segregation
CC       and cell cycle progression. Functions as a homodimer, which is
CC       essential for chromosome partition. Involved in negative DNA
CC       supercoiling in vivo, and by this means organize and compact
CC       chromosomes. May achieve or facilitate chromosome segregation by
CC       condensation DNA from both sides of a centrally located replisome
CC       during cell division. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- SUBUNIT: Homodimerization via its hinge domain. Binds to DNA via its C-
CC       terminal region. Interacts, and probably forms a ternary complex, with
CC       MukE and MukF via its C-terminal region. The complex formation is
CC       stimulated by calcium or magnesium. Interacts with tubulin-related
CC       protein FtsZ. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid {ECO:0000255|HAMAP-
CC       Rule:MF_01800}. Note=Restricted to the nucleoid region.
CC       {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- DOMAIN: The hinge domain, which separates the large intramolecular
CC       coiled coil regions, allows the homodimerization, forming a V-shaped
CC       homodimer. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- SIMILARITY: Belongs to the SMC family. MukB subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ417690; CAD10420.1; -; Genomic_DNA.
DR   RefSeq; WP_025298387.1; NZ_CP065604.1.
DR   AlphaFoldDB; Q93IE8; -.
DR   SMR; Q93IE8; -.
DR   STRING; 714.ACT75_00190; -.
DR   eggNOG; COG3096; Bacteria.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR   GO; GO:0009295; C:nucleoid; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.70.3500; -; 1.
DR   HAMAP; MF_01800; MukB; 1.
DR   InterPro; IPR012090; MukB.
DR   InterPro; IPR032520; MukB_hinge.
DR   InterPro; IPR042501; MukB_hinge_sf.
DR   InterPro; IPR007406; MukB_N_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF04310; MukB; 1.
DR   Pfam; PF16330; MukB_hinge; 1.
DR   PIRSF; PIRSF005246; MukB; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
PE   3: Inferred from homology;
KW   ATP-binding; Cell cycle; Cell division; Chromosome partition; Coiled coil;
KW   Cytoplasm; DNA condensation; DNA-binding; Nucleotide-binding.
FT   CHAIN           1..1496
FT                   /note="Chromosome partition protein MukB"
FT                   /id="PRO_0000068213"
FT   REGION          693..810
FT                   /note="Flexible hinge"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          332..496
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          537..608
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          665..692
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          806..832
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          862..1066
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          1101..1139
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          1235..1290
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   BINDING         61..68
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
SQ   SEQUENCE   1496 AA;  171557 MW;  057E83CA7DE9094C CRC64;
     MTEELSLEND VMYTTAEAAP VIFSQNSGVE RGKFRSLTLI NWNGFFARTF DLDELVTTLS
     GGNGAGKSTT MAGFVTALIP DLTLLHFRNT TEAGATSGSR DKGLHGKLRP GVCYAALDTI
     NSRHQRIIVG VRLQQVAGRD KKVDLKTFSI QGVELSVNPT ALFTETLNER QARVLTLNEL
     KDKVEQSGAQ FKQYHSITDY HGMMFDLGII PKRLRSSSDR SKFYKLIEAS LYGGISSAIT
     RSLRDYLLPE NLGVKKAFQD MESALRENRM TLEAIKVTQA DRDLFKHLIT ESTNYVAADY
     MRNANERRGN IESALNFRQE WYKAKSEQDL SQHRLVDLSR EAAELAENEK TLEVDHQSAL
     DHLNLVLNAL RHQEKISRYQ ENVGELTERL EEQKMVVETA NEQLEESQAQ FEQTEQEVDN
     LRSQLADYQQ ALDAQQTRAL QYQQAIQALE KAKTLCGLAD LAVKNVDVYH EEFEAQAEVL
     TDKVLELEQK MSISEAAKTQ FDKAYQLVCK IVGDVPRSAA WESAKELLRE YPTQKLQAQQ
     TPQLRAKLHE LEQRLNQQQS AVRLLNDFNQ RANLSLETAD ELEEFYGEQE ALIEDLSAEL
     SDLVVQRSTF RQKRENLTAL YEENARKAPA WLTAQAALER LQDQSGEQFA DSQGVMNFMQ
     AQLVKEREFT MERDQLELQR QQLDEQISRL SQPDGSEDAR LNVLAERFGG VLLSELYDDV
     PIEDAPYFSA LYGPARHAIV VRDLNAVKEQ LANLEDCPED LYLIEGDPAA FDDSVLSAQE
     LELGVVVQVS DRELRYSKFP EIPLFGRAAR EKHLEELQAK REEVAEHYAQ RAFDVQKCQR
     LHEHFSQFVG LHLALAFQDN PEQVMARTNQ QRNEIERELN QFLTGEQQIR IRLDDAKERM
     QLLNKLIPQL PLLADDSLTD RIEECREQLD LAEQDELFIR QYGVTLSQLE PIANTLQSDP
     ENYEHLKADY EQAIQLQKQV QQKVFALADV VQRKAHFNYA ESVQTETSEL NEQLRARLEQ
     MQQQRETQRE QLRQVQAQYA QYNQVLIQLQ SSFNSKNQML QELMQEIGEL GVRADEGAEE
     RAKIRRDELY QQLSTNRQCR SYIEKQLTLI ESEAENLTRR IRKAERDYKT QRELVTAAKM
     SWCVVLRLSR NSDVEKRLNR RELAYLSADE LRSMSDKALG ALRTAVADNE YLRDALRLSE
     DSRKPENKVR FFIAVYQHLR ERIRQDILKT DDPIDAIEQM EIELSRLTEE LTGREQKLAI
     SSESVANIMR KTIQREQNRI RMLNQGLQNI AFGQVKSVRL VVNIRDTHAM LLDALSGNQS
     DYQDLFTDNR MTFSEAIAKL YQRLNPHIDV GQRTAQTIGE ELLDYRNYLD LEVEVYRGAD
     GWLRAESGAL STGEAIGTGM SILLMVVQSW EEESRRIRGK DIVPCRLLFL DEAARLDAKS
     ISTLFELCER LDMQLLIAAP ENISPEKGTT YKLVRKISGN HEHVHVVGLR GFGTTE