ID   MUKB_EDWI9              Reviewed;        1485 AA.
AC   C5BAC7;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Chromosome partition protein MukB {ECO:0000255|HAMAP-Rule:MF_01800};
DE   AltName: Full=Structural maintenance of chromosome-related protein {ECO:0000255|HAMAP-Rule:MF_01800};
GN   Name=mukB {ECO:0000255|HAMAP-Rule:MF_01800}; OrderedLocusNames=NT01EI_2437;
OS   Edwardsiella ictaluri (strain 93-146).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Hafniaceae; Edwardsiella.
OX   NCBI_TaxID=634503;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=93-146;
RA   Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C.,
RA   Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.;
RT   "Complete genome sequence of Edwardsiella ictaluri 93-146.";
RL   Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a central role in chromosome condensation, segregation
CC       and cell cycle progression. Functions as a homodimer, which is
CC       essential for chromosome partition. Involved in negative DNA
CC       supercoiling in vivo, and by this means organize and compact
CC       chromosomes. May achieve or facilitate chromosome segregation by
CC       condensation DNA from both sides of a centrally located replisome
CC       during cell division. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- SUBUNIT: Homodimerization via its hinge domain. Binds to DNA via its C-
CC       terminal region. Interacts, and probably forms a ternary complex, with
CC       MukE and MukF via its C-terminal region. The complex formation is
CC       stimulated by calcium or magnesium. Interacts with tubulin-related
CC       protein FtsZ. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid {ECO:0000255|HAMAP-
CC       Rule:MF_01800}. Note=Restricted to the nucleoid region.
CC       {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- DOMAIN: The hinge domain, which separates the large intramolecular
CC       coiled coil regions, allows the homodimerization, forming a V-shaped
CC       homodimer. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- SIMILARITY: Belongs to the SMC family. MukB subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01800}.
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DR   EMBL; CP001600; ACR69607.1; -; Genomic_DNA.
DR   RefSeq; WP_015871723.1; NC_012779.2.
DR   AlphaFoldDB; C5BAC7; -.
DR   SMR; C5BAC7; -.
DR   STRING; 67780.B6E78_04330; -.
DR   EnsemblBacteria; ACR69607; ACR69607; NT01EI_2437.
DR   GeneID; 7959169; -.
DR   KEGG; eic:NT01EI_2437; -.
DR   PATRIC; fig|634503.3.peg.2160; -.
DR   HOGENOM; CLU_004430_0_0_6; -.
DR   OMA; FIAVYQH; -.
DR   OrthoDB; 331846at2; -.
DR   Proteomes; UP000001485; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR   GO; GO:0009295; C:nucleoid; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.70.3500; -; 1.
DR   HAMAP; MF_01800; MukB; 1.
DR   InterPro; IPR012090; MukB.
DR   InterPro; IPR032520; MukB_hinge.
DR   InterPro; IPR042501; MukB_hinge_sf.
DR   InterPro; IPR007406; MukB_N_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF04310; MukB; 1.
DR   Pfam; PF16330; MukB_hinge; 1.
DR   PIRSF; PIRSF005246; MukB; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
PE   3: Inferred from homology;
KW   ATP-binding; Cell cycle; Cell division; Chromosome partition; Coiled coil;
KW   Cytoplasm; DNA condensation; DNA-binding; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..1485
FT                   /note="Chromosome partition protein MukB"
FT                   /id="PRO_1000215936"
FT   REGION          666..783
FT                   /note="Flexible hinge"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          311..480
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          519..665
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          832..1115
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          1209..1265
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   BINDING         34..41
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
SQ   SEQUENCE   1485 AA;  168776 MW;  F54F7BDAABB73AA8 CRC64;
     MIERGKFRSL TLVNWNGFFA RTFDLDELVT TLSGGNGAGK STTMAAFVTA LIPDLTLLHF
     RNTTEAGATS GSRDKGLHGK LRPGVCYAAL DVVNSRHQRV LVGVRLQQVA GRDRKVDIKP
     FAIQGLPTAY QPTQLLTETV DGRQARVLAL PELKERVEAI DGVQFKQFNS ITDYHALMFD
     LGVVPRRLRS AADRSKFYRL IEASLYGGIS SAITRSLRDY LLPENGGVRK AFQDMEAALR
     ENRMTLEAIR VTQSDRDLFK HLISEATAYV SADYMRHANE RRSHLDQALQ LRRELLGGRR
     QLLSEQYRHV EMARELEEQN GAEADLETDY QAASDHLNLV QTALRQREKI ERYQGDLEEL
     SYRLDEQSEV AAEAQEQYES CQERSEAAEA EVDELKSQLA DYQQALDVQQ TRAIQYQQAL
     QALARAQTLC TLPALSADDV ESWLEDFRAR EEEATELLLQ LEQKLSVANA AHSQFEEAYR
     LVARVVGEVS RSDAWQAGRA LLREWPALQH QAQRVAPIGA RLAELEQRLN AQQDAERLLQ
     ECVKRSGRDC DAQQLDALQA ELEAQIDTLT QQAADAGERS MALRQELEQI AGRVGTLSAR
     APAWIAAQEA LNALESQCGE TLEESRAVTD QMQQLLERER EYTVERDEIA ARKGALEREI
     ERLSQPGGAE DARLIALAER FGGVLLSEIY DDVTLDDAPY FSALYGPSRH AIVVPDLAAV
     RAQLAGLEEC PEDLYLIEGD PQSFDDSVFA VEELEKAVVV KIADRQWRYS RFPSLPLFGR
     AARESRLETL YTEREALAER YATLSFDVQK IQRLHHAFSR FIGSHLAVVF DNDPEQELRQ
     LQQRRGEIER EFTGQDAQTQ QQRQQLQQAR ELAGLLNRLV PQVGLLADET LPDRVDELRE
     ELEQARDAAR YLQQHGATLA ALEPVIGVLQ SDPQQHEQLQ QDYQQALQRQ RLTKQQAFAL
     TEVAQRRAHF SYSDAVGMLG ENADLNERLR QRLEHAEADR SRSREQLRQH QAQLAQYNQV
     LASLKSAFDA KRDMLQELQQ EMQDIGVVAD ASAEARARTR RDELHAALSE NRSRRNQLEK
     QITICEAEME SLIKRLRKAE RDYHLMRTQV TQAKAGWCAV MRLVRDNGVE RRLHRRELAY
     MDADELRSMS DKALGALRLA VADNEHLRDV LRLSEDPKRP ERKVQFYVAV YQHLRERIRQ
     DIIRSDDPID AIEQMEIELA RLTEELTARE QKLAISARSV ANIIRKTIQR EQNRIRMLNQ
     GLQSVAFGQV NSVRLNVNVR DSHAMLLNVL SEQQEQHQDL FNSNRLTFSE ALAKLYQRLN
     PQIDMGQRTP QTIGEELLDY RSYLEMEVEV NRGSDGWLRA ESGALSTGEA IGTGMSILVM
     VVQSWEEESR RLRGKDISPC RLLFLDEAAR LDAKSIATLF ELCERLEMQL IIAAPENISP
     EKGTTYKLVR KVFNHIEHVH VVGLRGFSAA AEEEHMAQPL EDTPA