ID   MUKB_ENT38              Reviewed;        1482 AA.
AC   A4W8U3;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Chromosome partition protein MukB {ECO:0000255|HAMAP-Rule:MF_01800};
DE   AltName: Full=Structural maintenance of chromosome-related protein {ECO:0000255|HAMAP-Rule:MF_01800};
GN   Name=mukB {ECO:0000255|HAMAP-Rule:MF_01800}; OrderedLocusNames=Ent638_1443;
OS   Enterobacter sp. (strain 638).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Enterobacter.
OX   NCBI_TaxID=399742;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=638;
RX   PubMed=20485560; DOI=10.1371/journal.pgen.1000943;
RA   Taghavi S., van der Lelie D., Hoffman A., Zhang Y.B., Walla M.D.,
RA   Vangronsveld J., Newman L., Monchy S.;
RT   "Genome sequence of the plant growth promoting endophytic bacterium
RT   Enterobacter sp. 638.";
RL   PLoS Genet. 6:E1000943-E1000943(2010).
CC   -!- FUNCTION: Plays a central role in chromosome condensation, segregation
CC       and cell cycle progression. Functions as a homodimer, which is
CC       essential for chromosome partition. Involved in negative DNA
CC       supercoiling in vivo, and by this means organize and compact
CC       chromosomes. May achieve or facilitate chromosome segregation by
CC       condensation DNA from both sides of a centrally located replisome
CC       during cell division. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- SUBUNIT: Homodimerization via its hinge domain. Binds to DNA via its C-
CC       terminal region. Interacts, and probably forms a ternary complex, with
CC       MukE and MukF via its C-terminal region. The complex formation is
CC       stimulated by calcium or magnesium. Interacts with tubulin-related
CC       protein FtsZ. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid {ECO:0000255|HAMAP-
CC       Rule:MF_01800}. Note=Restricted to the nucleoid region.
CC       {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- DOMAIN: The hinge domain, which separates the large intramolecular
CC       coiled coil regions, allows the homodimerization, forming a V-shaped
CC       homodimer. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- SIMILARITY: Belongs to the SMC family. MukB subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01800}.
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DR   EMBL; CP000653; ABP60123.1; -; Genomic_DNA.
DR   RefSeq; WP_012016840.1; NC_009436.1.
DR   AlphaFoldDB; A4W8U3; -.
DR   SMR; A4W8U3; -.
DR   STRING; 399742.Ent638_1443; -.
DR   PRIDE; A4W8U3; -.
DR   EnsemblBacteria; ABP60123; ABP60123; Ent638_1443.
DR   KEGG; ent:Ent638_1443; -.
DR   eggNOG; COG3096; Bacteria.
DR   HOGENOM; CLU_004430_0_0_6; -.
DR   OMA; FIAVYQH; -.
DR   OrthoDB; 331846at2; -.
DR   Proteomes; UP000000230; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR   GO; GO:0009295; C:nucleoid; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.70.3500; -; 1.
DR   HAMAP; MF_01800; MukB; 1.
DR   InterPro; IPR012090; MukB.
DR   InterPro; IPR032520; MukB_hinge.
DR   InterPro; IPR042501; MukB_hinge_sf.
DR   InterPro; IPR007406; MukB_N_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF04310; MukB; 1.
DR   Pfam; PF16330; MukB_hinge; 1.
DR   PIRSF; PIRSF005246; MukB; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
PE   3: Inferred from homology;
KW   ATP-binding; Cell cycle; Cell division; Chromosome partition; Coiled coil;
KW   Cytoplasm; DNA condensation; DNA-binding; Nucleotide-binding.
FT   CHAIN           1..1482
FT                   /note="Chromosome partition protein MukB"
FT                   /id="PRO_1000069906"
FT   REGION          666..783
FT                   /note="Flexible hinge"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          326..416
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          509..603
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          780..805
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          835..923
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          979..1116
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          1210..1265
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   BINDING         34..41
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
SQ   SEQUENCE   1482 AA;  169184 MW;  3846AEFBF5468CFF CRC64;
     MIERGKFRSL TLINWNGFFA RTFDLDELVT TLSGGNGAGK STTMAAFVTA LIPDLTLLHF
     RNTTEAGATS GSRDKGLHGK LKAGVCYSVL DVINSRHQRV VVGVRLQQVA GRDRKVDIKP
     FAIQGLPTSV QPTAMLTETL NDRQARVLSL QELKEKLEEI EGVQFKQFNS ITDYHSLMFD
     LGIVARRLRS ASDRSKYYRL IEASLYGGIS SAITRSLRDY LLPENSGVRK AFQDMEAALR
     ENRMTLEAIR VTQSDRDLFK HLISEATNYV AADYMRHANE RRIHLDKALE YRRELFTSRK
     QLVAEQYKHV EMARELGEQN GAEGDLEADY QAASDHLNLV QTALRQQEKI ERYESDLDEL
     QIRLEEQNEV VAEATEMQEE NEARAEAAEL EVDELKNQLA DYQQALDVQQ TRAIQYTQAL
     QALQRAKELC HLPDLTPESA DEWLETFQAK QQEATDKLLS LDQKMSVAQT AHSQFEQAYQ
     LVTAINGPLA RSDAWEVARE LLRDGVNQRH LAEQVQPLRM RLNELEQRLR EQQEAERLLA
     EFCKRYGKRV DIDDLEALHQ ELEARIASLS DSVSNAGEQR MSLRQELEQL QSRTQKLLQR
     APVWLAAQSS LNQLSEQCGE EFESSQDVTE YLQQLLERER EAIVERDEVG ARKRAIDEEI
     ERLSQPGGAE DSRLNALAER FGGVLLSEIY DDVSLDDAPY FSALYGPSRN AIVVPDLSLI
     ADQLEGLEDC PEDLYLIEGD PQSFDDSVFS VDELEKAVVV KIADRQWRYS RFPTLPLFGR
     AARENRIESL HAERETLSER FATLSFDVQK TQRLHQSFSR FIGSHVAVAF EPDPEAEIRK
     LNTRRGELER AISLHENDNQ QSRVQFEQAK EGVAALNRIL PRLNLLADET LADRVDEIQE
     RLDEAQEAAR FVQQHGNQLA KLEPVLSVLQ SDPEQFEQLK EDYAYSQQVQ RDARQQAFAL
     AEVVQRRAHF GYSDSAEMLS GNSDLNEKLR HRLEQAETER SRTRDALRSH AAQLSQYGQV
     LASLKSSFDT KKELLTDLQK ELQDIGVRAD SGAEERARIR RDELHSQLSN NRSRRNQLEK
     ALTLCEAEMD NLTRRLRKLE RDYHEMREQV VTAKAGWCAV MRLVKDSGVE RRLHRRELAY
     LSGDELRSMS DKALGALRPA VADNEHLRDV LRMSEDPKRP ERKIQFFVAV YQHLRERIRQ
     DIIRTDDPVE AIEQMEIELG RLTEELTSRE QTLAISSRSV ANIIRKTIQR EQNRIRMLNQ
     GLQSVSFGQV NSVRLNVNVL EAHATLLDVL SEQHEQHQDL FNSNRLTFSE ALAKLYQRLN
     PQIDMGQRTP QTIGEELLDY RNYLEMEVEV NRGSDGWLRA ESGALSTGEA IGTGMSILVM
     VVQSWEDESR RLRGKDISPC RLLFLDEAAR LDARSIATLF ELCDRLGMQL IIAAPENISP
     EKGTTYKLVR KVFQNTEHVH VVGLRGFAPQ PPESLPEAAD AS