ID   MUKB_KLEP3              Reviewed;        1482 AA.
AC   B5XY73;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Chromosome partition protein MukB {ECO:0000255|HAMAP-Rule:MF_01800};
DE   AltName: Full=Structural maintenance of chromosome-related protein {ECO:0000255|HAMAP-Rule:MF_01800};
GN   Name=mukB {ECO:0000255|HAMAP-Rule:MF_01800}; OrderedLocusNames=KPK_3608;
OS   Klebsiella pneumoniae (strain 342).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=507522;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=342;
RX   PubMed=18654632; DOI=10.1371/journal.pgen.1000141;
RA   Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H.,
RA   Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y.,
RA   Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W.,
RA   Methe B.A.;
RT   "Complete genome sequence of the N2-fixing broad host range endophyte
RT   Klebsiella pneumoniae 342 and virulence predictions verified in mice.";
RL   PLoS Genet. 4:E1000141-E1000141(2008).
CC   -!- FUNCTION: Plays a central role in chromosome condensation, segregation
CC       and cell cycle progression. Functions as a homodimer, which is
CC       essential for chromosome partition. Involved in negative DNA
CC       supercoiling in vivo, and by this means organize and compact
CC       chromosomes. May achieve or facilitate chromosome segregation by
CC       condensation DNA from both sides of a centrally located replisome
CC       during cell division. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- SUBUNIT: Homodimerization via its hinge domain. Binds to DNA via its C-
CC       terminal region. Interacts, and probably forms a ternary complex, with
CC       MukE and MukF via its C-terminal region. The complex formation is
CC       stimulated by calcium or magnesium. Interacts with tubulin-related
CC       protein FtsZ. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid {ECO:0000255|HAMAP-
CC       Rule:MF_01800}. Note=Restricted to the nucleoid region.
CC       {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- DOMAIN: The hinge domain, which separates the large intramolecular
CC       coiled coil regions, allows the homodimerization, forming a V-shaped
CC       homodimer. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- SIMILARITY: Belongs to the SMC family. MukB subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01800}.
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DR   EMBL; CP000964; ACI07726.1; -; Genomic_DNA.
DR   AlphaFoldDB; B5XY73; -.
DR   SMR; B5XY73; -.
DR   EnsemblBacteria; ACI07726; ACI07726; KPK_3608.
DR   KEGG; kpe:KPK_3608; -.
DR   HOGENOM; CLU_004430_0_0_6; -.
DR   OMA; FIAVYQH; -.
DR   OrthoDB; 331846at2; -.
DR   Proteomes; UP000001734; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR   GO; GO:0009295; C:nucleoid; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.70.3500; -; 1.
DR   HAMAP; MF_01800; MukB; 1.
DR   InterPro; IPR012090; MukB.
DR   InterPro; IPR032520; MukB_hinge.
DR   InterPro; IPR042501; MukB_hinge_sf.
DR   InterPro; IPR007406; MukB_N_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF04310; MukB; 1.
DR   Pfam; PF16330; MukB_hinge; 1.
DR   PIRSF; PIRSF005246; MukB; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
PE   3: Inferred from homology;
KW   ATP-binding; Cell cycle; Cell division; Chromosome partition; Coiled coil;
KW   Cytoplasm; DNA condensation; DNA-binding; Nucleotide-binding.
FT   CHAIN           1..1482
FT                   /note="Chromosome partition protein MukB"
FT                   /id="PRO_1000187480"
FT   REGION          666..783
FT                   /note="Flexible hinge"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   REGION          1056..1075
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          326..472
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          507..602
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          832..1110
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          1209..1265
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   BINDING         34..41
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
SQ   SEQUENCE   1482 AA;  169754 MW;  B72AB94C80C2979F CRC64;
     MIERGKFRSL TLVNWNGFFA RTFDLDELVT TLSGGNGAGK STTMAAFVTA LIPDLTLLHF
     RNTTEAGATS GSRDKGLHGK LRAGVCYSVL DVINSRHQRV VVGVRLQQVA GRDRKVDIKP
     FAIQGLPTSI LPTQLLTETL NDRQARVVSL NELKDKLEAM EGVQFKQFNS ITEYHSLMFD
     LGVVARRLRS ASDRSKYYRL IEASLYGGIS STITRSLRDY LLPENSGVRK AFQDMEAALR
     ENRMTLEAIR VTQSDRDLFK HLISEATNYV AADYMRHANE RRIHLDKALE YRRDLFTSRS
     QLAAEQYKHV EMARELQEHN GAEGDLEADY QAASDHLNLV QTALRQQEKI ERYEADLDEL
     QIRLEEQNEV VAEAADQQEE NEARAEAAEL EVDELKNQLA DYQQALDVQQ TRAIQYNQAL
     QALERAKTLC HLPDLTPESA DEWLETFQAK EQEATEKMLS LEQKMSVAQT AHSQFEQAYQ
     LVAAINGPLA RNEAWDVARE LLRDGVNQRH QAEQAQGLRS RLNELEQRLR EQQDAERQLA
     EFCKRQGKRY DIDDLETLHQ ELEARIASLA DSVSNAQEQR MTLRQELEQL QSRTQTLMRR
     APIWLAAQNS LNQLCEQSGE QFESGQEVTE YLQQLLERER EAIVERDEVG ARKRAIDEEI
     ERLSQPGGSE DPRLNALAER FGGVLLSEIY DDVSLEDAPY FSALYGPSRH AIVVPDLSQV
     AEQLEGLEDC PEDLYLIEGD PQSFDDSVFS VDELEKAVVV KIADRQWRYS RFPTLPLFGR
     AARENRIETL HAERESLSER FATLSFDVQK TQRLHQAFSR FIGSHLAVAF EDDPEEEIRK
     LNSRRGELER ALNAHESDNQ QNRVQYEQAK EGVSALNRLL PRLNLLADDT LADRVDEIQE
     RLDEAQEAVR FIQQHGNQLA KLEPIVSVLQ SDPEQFEQLK EDYAYAQQTQ RDARQQAFAL
     AEVVQRRAHF SYSDSAEMLS GNSDLNEKLR QRLQQAESER SRAREAMRSH VAQLNQYNQV
     LASLKSSYDT KKELLNDLYK ELQDIGVRAD AGAEERARQR RDELHTQLSN NRSRRNQLEK
     ALTFCEAEMD NLTRKLRKLE RDYCEMREQV VSAKAGWCAV MRLVKDNGVE RRLHRRELAY
     LSADELRSMS DKALGALRLA VADNEHLRDV LRISEDPKRP ERKIQFFVAV YQHLRERIRQ
     DIIRTDDPVE AIEQMEIELS RLTEELTNRE QKLAISSRSV ANIIRKTIQR EQNRIRMLNQ
     GLQSVSFGQV NSVRLNVNVR ETHSTLLDVL SEQHEQHQDL FNSNRLTFSE ALAKLYQRLN
     PQIDMGQRTP QTIGEELLDY RNYLEMEVEV NRGSDGWLRA ESGALSTGEA IGTGMSILVM
     VVQSWEDESR RLRGKDISPC RLLFLDEAAR LDARSIATLF ELCERLEMQL IIAAPENISP
     EKGTTYKLVR KVFNNHEHVH VVGLRGFAAP VPEALPGTAD AS