MUKB_VIBC3
ID MUKB_VIBC3 Reviewed; 1491 AA.
AC A5F7H8; C3M1B2;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Chromosome partition protein MukB {ECO:0000255|HAMAP-Rule:MF_01800};
DE AltName: Full=Structural maintenance of chromosome-related protein {ECO:0000255|HAMAP-Rule:MF_01800};
GN Name=mukB {ECO:0000255|HAMAP-Rule:MF_01800};
GN OrderedLocusNames=VC0395_A1317, VC395_1831;
OS Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS O395).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=345073;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA Heidelberg J.;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA Wang W., Wang J., Qian W., Li D., Wang L.;
RT "A recalibrated molecular clock and independent origins for the cholera
RT pandemic clones.";
RL PLoS ONE 3:E4053-E4053(2008).
CC -!- FUNCTION: Plays a central role in chromosome condensation, segregation
CC and cell cycle progression. Functions as a homodimer, which is
CC essential for chromosome partition. Involved in negative DNA
CC supercoiling in vivo, and by this means organize and compact
CC chromosomes. May achieve or facilitate chromosome segregation by
CC condensation DNA from both sides of a centrally located replisome
CC during cell division. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC -!- SUBUNIT: Homodimerization via its hinge domain. Binds to DNA via its C-
CC terminal region. Interacts, and probably forms a ternary complex, with
CC MukE and MukF via its C-terminal region. The complex formation is
CC stimulated by calcium or magnesium. Interacts with tubulin-related
CC protein FtsZ. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid {ECO:0000255|HAMAP-
CC Rule:MF_01800}. Note=Restricted to the nucleoid region.
CC {ECO:0000255|HAMAP-Rule:MF_01800}.
CC -!- DOMAIN: The hinge domain, which separates the large intramolecular
CC coiled coil regions, allows the homodimerization, forming a V-shaped
CC homodimer. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC -!- SIMILARITY: Belongs to the SMC family. MukB subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01800}.
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DR EMBL; CP000627; ABQ20826.1; -; Genomic_DNA.
DR EMBL; CP001235; ACP09828.1; -; Genomic_DNA.
DR RefSeq; WP_000572778.1; NZ_JAACZH010000016.1.
DR AlphaFoldDB; A5F7H8; -.
DR SMR; A5F7H8; -.
DR STRING; 345073.VC395_1831; -.
DR EnsemblBacteria; ABQ20826; ABQ20826; VC0395_A1317.
DR KEGG; vco:VC0395_A1317; -.
DR KEGG; vcr:VC395_1831; -.
DR PATRIC; fig|345073.21.peg.1774; -.
DR eggNOG; COG3096; Bacteria.
DR HOGENOM; CLU_004430_0_0_6; -.
DR OMA; FIAVYQH; -.
DR Proteomes; UP000000249; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR GO; GO:0009295; C:nucleoid; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.70.3500; -; 1.
DR HAMAP; MF_01800; MukB; 1.
DR InterPro; IPR012090; MukB.
DR InterPro; IPR032520; MukB_hinge.
DR InterPro; IPR042501; MukB_hinge_sf.
DR InterPro; IPR007406; MukB_N_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF04310; MukB; 1.
DR Pfam; PF16330; MukB_hinge; 1.
DR PIRSF; PIRSF005246; MukB; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
PE 3: Inferred from homology;
KW ATP-binding; Cell cycle; Cell division; Chromosome partition; Coiled coil;
KW Cytoplasm; DNA condensation; DNA-binding; Nucleotide-binding.
FT CHAIN 1..1491
FT /note="Chromosome partition protein MukB"
FT /id="PRO_1000073658"
FT REGION 667..784
FT /note="Flexible hinge"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT REGION 1059..1080
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 302..418
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT COILED 488..600
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT COILED 638..666
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT COILED 781..806
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT COILED 836..1109
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT COILED 1210..1239
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT BINDING 34..41
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
SQ SEQUENCE 1491 AA; 169898 MW; 51D4EBD8EAF4DD14 CRC64;
MIERGKYQSL TMINWNGFFA RTFDIDNLVT TLSGGNGAGK STTMAAFITA LIPDQSLLHF
RNTTEAGSSQ ASRDKGLYGK LQAGACYAAL DVVNSRNQRL LFAVKLQQVA GRDKKVDIKP
FLIQGLPSHV KPTDVLVETV SDKHARVRQI NEVKDAVGQI EGAHFKSFPS IVDYHAQMFE
FGVIPKKLRN SSDRSKFYRL IEASLYGGIS SAITRSLRDY LLPQNGGVKK AFQDMESALR
ENRMTLEAIK TTQADRDLFK HLITESTNYV AADYMRHAND RRNKVGQTLV LRGELFSSRE
TLIEQNSLLN RVHEELELLV EQESALEQDY QGASDHLQLV QNALRQQEKI ERYQEDLEEL
NFRLEEQMMV VEEANERVMM AEERATISEE EVDSLKSQLA DYQQALDVQQ TRALQYQQAV
QALDKARRLL DKPELTAESA QALATQLKAE QETRTSELLA LKHKLDMSSA AAQQFNHAFD
LVKRVLGEVA RSEASKQAQQ VIRQAREAQN VLQNEAQWQA QQRDLERQLE QQRSVRELAT
QYHKQHMVAL DDAATVELER ERHSALLEEL ETEQENCREQ RGQLRHQEQE LQTQIARFES
IAPAWIKAND ALETLREQSG AELADSQSVM AHMQQVLELE KAQSMAKDKL AERRAKLDSE
IERLASPGGS NDPRLKGLAD TLGGVLLSEI YDDITIDDAP YFSAMYGPAR HAIVVSDLSG
IKEKLVELDD CPEDLYLIEG DVDAFDDSSF NAEELEGAVC VQLNQRQMRY SRFPAIPLFG
RAAREQRLEL LREERDDVVE QHAKASFDSQ KLQRLYASFN QFVAMHLQVA FDADPEQALA
NARDKRNQLL RSISEFEAQE QQLRSQLQAS KQALAALDKL APQMGLLDEE TLEARYQELE
EKLQQLSEAK AFIAAHGRTI SELEKVAAVL DADPEQFDAL EQQYQQADQA LQQLKAQIFA
LSDLLERRHH FAYSDSVDLL NQSSELSEQL KAKLVQAESE RTRSREELKQ AQAQLSQYNQ
LLASLKSSHQ AKLETVQEFK QELQEFGVHA DEGAIERAQR RRDELQERLH TSRSRKSEYE
RTITSTELEM KALVKRMKKV EKDYQDLRTF VVNAKAGWCS VLRLARQNDV ERRLHKRELA
YLSADELRSM SDKSLGALRL AVANNEDLRD ALRQSEDNSR PERKVLFYIA VYQHLRERIR
QDIIRTDDPV EAIEEMEVEL ARLTEELTQR EQRLAISSDS VASIIRKTIQ REQNRIRMLN
QGLSNISFGQ VNGVRLNVKV RESHEILLAG LSEQQAQHKD LFESARYTFS EAMAKLFQRV
NPHIDMGQRS PQVLGEELLD YRNYLELSVE VNRGSDGWLQ AESGALSTGE AIGTGQSILL
MVVQSWEEES RRLRSKDIVP CRLLFLDEAA RLDAKSIATL FELCERLDMQ LLIAAPENIS
PEKGTTYKLV RKVFKDHEHV HVVGLRGFAQ TEKPKTAEQK FAEELAGELT E
