ID   MUKB_VIBVY              Reviewed;        1487 AA.
AC   Q7MJ64;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Chromosome partition protein MukB {ECO:0000255|HAMAP-Rule:MF_01800};
DE   AltName: Full=Structural maintenance of chromosome-related protein {ECO:0000255|HAMAP-Rule:MF_01800};
GN   Name=mukB {ECO:0000255|HAMAP-Rule:MF_01800}; OrderedLocusNames=VV2298;
OS   Vibrio vulnificus (strain YJ016).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=196600;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YJ016;
RX   PubMed=14656965; DOI=10.1101/gr.1295503;
RA   Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L.,
RA   Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P.,
RA   Lee C.-T., Hor L.-I., Tsai S.-F.;
RT   "Comparative genome analysis of Vibrio vulnificus, a marine pathogen.";
RL   Genome Res. 13:2577-2587(2003).
CC   -!- FUNCTION: Plays a central role in chromosome condensation, segregation
CC       and cell cycle progression. Functions as a homodimer, which is
CC       essential for chromosome partition. Involved in negative DNA
CC       supercoiling in vivo, and by this means organize and compact
CC       chromosomes. May achieve or facilitate chromosome segregation by
CC       condensation DNA from both sides of a centrally located replisome
CC       during cell division. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- SUBUNIT: Homodimerization via its hinge domain. Binds to DNA via its C-
CC       terminal region. Interacts, and probably forms a ternary complex, with
CC       MukE and MukF via its C-terminal region. The complex formation is
CC       stimulated by calcium or magnesium. Interacts with tubulin-related
CC       protein FtsZ. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid {ECO:0000255|HAMAP-
CC       Rule:MF_01800}. Note=Restricted to the nucleoid region.
CC       {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- DOMAIN: The hinge domain, which separates the large intramolecular
CC       coiled coil regions, allows the homodimerization, forming a V-shaped
CC       homodimer. {ECO:0000255|HAMAP-Rule:MF_01800}.
CC   -!- SIMILARITY: Belongs to the SMC family. MukB subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01800}.
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DR   EMBL; BA000037; BAC95062.1; -; Genomic_DNA.
DR   RefSeq; WP_011150798.1; NC_005139.1.
DR   AlphaFoldDB; Q7MJ64; -.
DR   SMR; Q7MJ64; -.
DR   STRING; 672.VV93_v1c20100; -.
DR   EnsemblBacteria; BAC95062; BAC95062; BAC95062.
DR   KEGG; vvy:VV2298; -.
DR   PATRIC; fig|196600.6.peg.2309; -.
DR   eggNOG; COG3096; Bacteria.
DR   HOGENOM; CLU_004430_0_0_6; -.
DR   OMA; FIAVYQH; -.
DR   OrthoDB; 331846at2; -.
DR   Proteomes; UP000002675; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR   GO; GO:0009295; C:nucleoid; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.70.3500; -; 1.
DR   HAMAP; MF_01800; MukB; 1.
DR   InterPro; IPR012090; MukB.
DR   InterPro; IPR032520; MukB_hinge.
DR   InterPro; IPR042501; MukB_hinge_sf.
DR   InterPro; IPR007406; MukB_N_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF04310; MukB; 1.
DR   Pfam; PF16330; MukB_hinge; 1.
DR   PIRSF; PIRSF005246; MukB; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell cycle; Cell division; Chromosome partition; Coiled coil;
KW   Cytoplasm; DNA condensation; DNA-binding; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..1487
FT                   /note="Chromosome partition protein MukB"
FT                   /id="PRO_0000068232"
FT   REGION          667..784
FT                   /note="Flexible hinge"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          297..426
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          460..666
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          781..806
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          836..1111
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   COILED          1210..1266
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
FT   BINDING         34..41
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01800"
SQ   SEQUENCE   1487 AA;  169075 MW;  B097B6CCCC43ABED CRC64;
     MIERGKYQSL TMVNWNGFFA RTFDIDGLVT TLSGGNGAGK STTMAAFITA LIPDQSLLHF
     RNTTEAGSSQ ASRDKGLYGK LQPGACYAAL DVVNSRNQRL LFAVKLQQVA GRDKKVDIKP
     FVIQGLPSHV KPTDVLIESV SATQARVRQI NEVKESVAQY EGVQFKSFSS IVDYHAQMFE
     FGVIPKKLRN SSDRSKFYRL IEASLYGGIS SAITRSLRDY LLPQNGGVKK AFQDMESALR
     ENRMTLEAIK TTQADRDLFK HLITESTNYV AADYMRHANE RHKKLEQSLS LRSELFSSRE
     TLIEQNRLLN QVQQELEMLV ESESALEQDY QGASDHLQLV QNALRQQEKI ERYQEDLEEL
     NERLEEQSMV VEEAQERVLM AEEQSTVAEN EVDSLKTQLA DYQQALDVQQ TRALQYQQAV
     QALEKAKVLL SDSTLTAESA QALVAQLTQS EAEQTQALLA LKHKLDMSSA AAQQFENALK
     LVHSIAGQVE RAEASRYAKA AIQQARSAQQ VVQNENQWRA QHRDLERSLN QQNQAQALVA
     EYQKAHQVTL DDEVMFEQER ERHHAQLDSL EIALEENREL RSEQRRQEQD LQSDITQLQA
     IAPKWIAAND ALEKLREQSG AELADSQSVM SQMQQVLEQE KQLSQAKDKL AERRSQLESE
     IERLASPGGS NDPRLKGLAD TLGGVLLSEI YDDITIDDAP YFSAMYGPAR HAIVVSDLSG
     IEEKLVELDD CPEDLYIIEG DVDAFDDSSI KAEELEGAVC VRLNDRQMRY SRFPVIPLFG
     RAAREQRLEL LRSEREEVVE KHAKAAFDAQ KMQRLFQAFN QFVAEHIQVA FAADPEQALV
     IARDKRNQLT RTLAELEAKE QQMRSQIQNS KQALTMLDKL APMMAVISDD TIGERFAELE
     EKIAQLADAK QFLNAHAKAV EQLESQLAVL DADPEQFDAL EAQYQSADSQ LQALKKQIFA
     LSDLVERRHY FAYADSVDLL SKSSELSEQL KAKLVEAERT RSRYRDELKQ QQEQMNQYNQ
     VLASLKSSYQ AKLETVQEFK QELAEFGVSA DEGALERAIR RRDELHERLH TSRSRKSEYE
     RTLTSTELGM KELAKRLKKV QKEYVELRTF VVAAKAGWCS VLRLARENDV ERRLHKRELA
     YLSAGELRSM SDKSLGALRL AVANNDDLRD ALRLSEDNAR PERKVLFYIA VYQHLRERIR
     QDIIHTDDPV EAIEEMEVEL ARLTEELTMR ENRLAISSES VASIIKKTIQ REQNRIRMLN
     QGLSNISFGQ VKGVRLNVKV RESHEVLLNG LATQQEQHKD LFETARYTFS EAMAKLFQRV
     NPHIDMGQRS PQVLGEELLD YRNYLELSIE VNRGSDGWLQ AESGALSTGE AIGTGQSILL
     MVVQSWEEES RRLRSKDIVP CRLLFLDEAA RLDAKSISTL FELCDRLDMQ LLIAAPENIS
     PEKGTTYKLV RKVFKDHEHV HVVGLRGFGQ EQKPKSEAQQ MIEEFEA