MUKE_ECOLI
ID MUKE_ECOLI Reviewed; 234 AA.
AC P22524;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 24-NOV-2009, sequence version 5.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Chromosome partition protein MukE;
DE AltName: Full=Protein KicA;
GN Name=mukE; Synonyms=kicA, ycbA; OrderedLocusNames=b0923, JW0906;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=7513784; DOI=10.1007/bf00280310;
RA Feng J., Yamanaka K., Niki H., Ogura T., Hiraga S.;
RT "New killing system controlled by two genes located immediately upstream of
RT the mukB gene in Escherichia coli.";
RL Mol. Gen. Genet. 243:136-147(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP PROTEIN SEQUENCE OF 14-31; 68-78 AND 109-132, AND INTERACTION WITH MUKB.
RX PubMed=20921377; DOI=10.1073/pnas.1008678107;
RA Li Y., Stewart N.K., Berger A.J., Vos S., Schoeffler A.J., Berger J.M.,
RA Chait B.T., Oakley M.G.;
RT "Escherichia coli condensin MukB stimulates topoisomerase IV activity by a
RT direct physical interaction.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:18832-18837(2010).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 83-234.
RX PubMed=1989883; DOI=10.1002/j.1460-2075.1991.tb07935.x;
RA Niki H., Jaffe A., Imamura R., Ogura T., Hiraga S.;
RT "The new gene mukB codes for a 177 kd protein with coiled-coil domains
RT involved in chromosome partitioning of E. coli.";
RL EMBO J. 10:183-193(1991).
RN [7]
RP FUNCTION.
RX PubMed=8602138; DOI=10.1007/bf02174381;
RA Yamanaka K., Ogura T., Niki H., Hiraga S.;
RT "Identification of two new genes, mukE and mukF, involved in chromosome
RT partitioning in Escherichia coli.";
RL Mol. Gen. Genet. 250:241-251(1996).
RN [8]
RP CHARACTERIZATION, AND INTERACTION WITH MUKB AND MUKF.
RX PubMed=10545099; DOI=10.1093/emboj/18.21.5873;
RA Yamazoe M., Onogi T., Sunako Y., Niki H., Yamanaka K., Ichimura T.,
RA Hiraga S.;
RT "Complex formation of MukB, MukE and MukF proteins involved in chromosome
RT partitioning in Escherichia coli.";
RL EMBO J. 18:5873-5884(1999).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS), AND SUBUNIT.
RX PubMed=19135891; DOI=10.1016/j.cell.2008.10.050;
RA Woo J.-S., Lim J.-H., Shin H.-C., Suh M.-K., Ku B., Lee K.-H., Joo K.,
RA Robinson H., Lee J., Park S.-Y., Ha N.-C., Oh B.-H.;
RT "Structural studies of a bacterial condensin complex reveal ATP-dependent
RT disruption of intersubunit interactions.";
RL Cell 136:85-96(2009).
CC -!- FUNCTION: Involved in chromosome condensation, segregation and cell
CC cycle progression. May participate in facilitating chromosome
CC segregation by condensation DNA from both sides of a centrally located
CC replisome during cell division. Probably acts via its interaction with
CC MukB and MukF. {ECO:0000269|PubMed:8602138}.
CC -!- SUBUNIT: Forms a hexameric complex with MukF, (MukF-(MukE)2)2, which
CC forms a ternary complex with MukB. The complex formation is stimulated
CC by calcium or magnesium. {ECO:0000269|PubMed:19135891}.
CC -!- INTERACTION:
CC P22524; P60293: mukF; NbExp=6; IntAct=EBI-554672, EBI-554679;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid. Note=Restricted to the
CC nucleoid region.
CC -!- SIMILARITY: Belongs to the MukE family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA05458.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAA35669.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAA40775.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; D26440; BAA05458.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC74009.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA35669.2; ALT_INIT; Genomic_DNA.
DR EMBL; X57550; CAA40775.1; ALT_FRAME; Genomic_DNA.
DR PIR; B64832; B64832.
DR RefSeq; NP_415443.2; NC_000913.3.
DR RefSeq; WP_001295347.1; NZ_STEB01000006.1.
DR PDB; 3EUH; X-ray; 2.90 A; C/D/E/F=1-234.
DR PDB; 3RPU; X-ray; 3.60 A; D/E/G/H/Y/Z=10-234.
DR PDBsum; 3EUH; -.
DR PDBsum; 3RPU; -.
DR AlphaFoldDB; P22524; -.
DR SMR; P22524; -.
DR BioGRID; 4261869; 77.
DR BioGRID; 849924; 2.
DR ComplexPortal; CPX-1090; MukBEF condensin complex.
DR DIP; DIP-10274N; -.
DR IntAct; P22524; 11.
DR MINT; P22524; -.
DR STRING; 511145.b0923; -.
DR jPOST; P22524; -.
DR PaxDb; P22524; -.
DR PRIDE; P22524; -.
DR EnsemblBacteria; AAC74009; AAC74009; b0923.
DR EnsemblBacteria; BAA35669; BAA35669; BAA35669.
DR GeneID; 66670801; -.
DR GeneID; 945550; -.
DR KEGG; ecj:JW0906; -.
DR KEGG; eco:b0923; -.
DR PATRIC; fig|1411691.4.peg.1353; -.
DR EchoBASE; EB1232; -.
DR eggNOG; COG3095; Bacteria.
DR HOGENOM; CLU_1146408_0_0_6; -.
DR OMA; FLMDYQE; -.
DR PhylomeDB; P22524; -.
DR BioCyc; EcoCyc:EG11252-MON; -.
DR EvolutionaryTrace; P22524; -.
DR PRO; PR:P22524; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0000796; C:condensin complex; IPI:ComplexPortal.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR GO; GO:0009295; C:nucleoid; IEA:UniProtKB-SubCell.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0030261; P:chromosome condensation; IC:ComplexPortal.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.2250; -; 1.
DR Gene3D; 1.10.10.2260; -; 1.
DR HAMAP; MF_01802; MukE; 1.
DR InterPro; IPR042037; MukE_C.
DR InterPro; IPR042038; MukE_N.
DR InterPro; IPR007385; Scp_MukE.
DR Pfam; PF04288; MukE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell division; Chromosome partition; Cytoplasm;
KW Direct protein sequencing; DNA condensation; Reference proteome.
FT CHAIN 1..234
FT /note="Chromosome partition protein MukE"
FT /id="PRO_0000206792"
FT REGION 207..234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 12..19
FT /evidence="ECO:0007829|PDB:3EUH"
FT HELIX 23..31
FT /evidence="ECO:0007829|PDB:3EUH"
FT HELIX 41..59
FT /evidence="ECO:0007829|PDB:3EUH"
FT TURN 60..62
FT /evidence="ECO:0007829|PDB:3EUH"
FT STRAND 63..67
FT /evidence="ECO:0007829|PDB:3EUH"
FT STRAND 73..77
FT /evidence="ECO:0007829|PDB:3EUH"
FT STRAND 85..87
FT /evidence="ECO:0007829|PDB:3EUH"
FT HELIX 90..104
FT /evidence="ECO:0007829|PDB:3EUH"
FT HELIX 106..111
FT /evidence="ECO:0007829|PDB:3EUH"
FT STRAND 114..116
FT /evidence="ECO:0007829|PDB:3EUH"
FT HELIX 117..127
FT /evidence="ECO:0007829|PDB:3EUH"
FT HELIX 130..137
FT /evidence="ECO:0007829|PDB:3EUH"
FT STRAND 138..140
FT /evidence="ECO:0007829|PDB:3EUH"
FT HELIX 145..164
FT /evidence="ECO:0007829|PDB:3EUH"
FT STRAND 167..170
FT /evidence="ECO:0007829|PDB:3EUH"
FT STRAND 172..180
FT /evidence="ECO:0007829|PDB:3EUH"
FT HELIX 182..188
FT /evidence="ECO:0007829|PDB:3EUH"
FT HELIX 189..192
FT /evidence="ECO:0007829|PDB:3EUH"
FT STRAND 193..195
FT /evidence="ECO:0007829|PDB:3EUH"
FT HELIX 197..206
FT /evidence="ECO:0007829|PDB:3EUH"
FT STRAND 209..212
FT /evidence="ECO:0007829|PDB:3EUH"
SQ SEQUENCE 234 AA; 26974 MW; 1E771144C58207DD CRC64;
MSSTNIEQVM PVKLAQALAN PLFPALDSAL RSGRHIGLDE LDNHAFLMDF QEYLEEFYAR
YNVELIRAPE GFFYLRPRST TLIPRSVLSE LDMMVGKILC YLYLSPERLA NEGIFTQQEL
YDELLTLADE AKLLKLVNNR STGSDVDRQK LQEKVRSSLN RLRRLGMVWF MGHDSSKFRI
TESVFRFGAD VRAGDDPREA QRRLIRDGEA MPIENHLQLN DETEENQPDS GEEE
