MUM2_YEAST
ID MUM2_YEAST Reviewed; 366 AA.
AC P38236; D6VQ56;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Protein MUM2;
DE AltName: Full=Muddled meiosis protein 2;
GN Name=MUM2; OrderedLocusNames=YBR057C; ORFNames=YBR0514;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7597852; DOI=10.1002/yea.320110511;
RA Aljinovic G., Pohl T.M.;
RT "Sequence and analysis of 24 kb on chromosome II of Saccharomyces
RT cerevisiae.";
RL Yeast 11:475-479(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=12586695; DOI=10.1093/genetics/163.1.47;
RA Enyenihi A.H., Saunders W.S.;
RT "Large-scale functional genomic analysis of sporulation and meiosis in
RT Saccharomyces cerevisiae.";
RL Genetics 163:47-54(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP IDENTIFICATION IN THE MIS COMPLEX, AND FUNCTION.
RX PubMed=22685417; DOI=10.1371/journal.pgen.1002732;
RA Agarwala S.D., Blitzblau H.G., Hochwagen A., Fink G.R.;
RT "RNA methylation by the MIS complex regulates a cell fate decision in
RT yeast.";
RL PLoS Genet. 8:E1002732-E1002732(2012).
RN [7]
RP FUNCTION, IDENTIFICATION IN THE MIS COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=24269006; DOI=10.1016/j.cell.2013.10.047;
RA Schwartz S., Agarwala S.D., Mumbach M.R., Jovanovic M., Mertins P.,
RA Shishkin A., Tabach Y., Mikkelsen T.S., Satija R., Ruvkun G., Carr S.A.,
RA Lander E.S., Fink G.R., Regev A.;
RT "High-resolution mapping reveals a conserved, widespread, dynamic mRNA
RT methylation program in yeast meiosis.";
RL Cell 155:1409-1421(2013).
CC -!- FUNCTION: Component of the MIS complex, a complex that mediates N6-
CC methyladenosine (m6A) methylation on some mRNAs during meiosis and is
CC required for sporulation. {ECO:0000269|PubMed:22685417,
CC ECO:0000269|PubMed:24269006}.
CC -!- SUBUNIT: Component of the MIS complex, composed of SLZ1, MUM2 and IME4.
CC {ECO:0000269|PubMed:22685417, ECO:0000269|PubMed:24269006}.
CC -!- INTERACTION:
CC P38236; P41833: IME4; NbExp=3; IntAct=EBI-11619, EBI-9210;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24269006}. Nucleus,
CC nucleolus {ECO:0000269|PubMed:24269006}. Note=Localizes in the
CC nucleolus during the period of m6A mRNA accumulation.
CC -!- DISRUPTION PHENOTYPE: Mutant is sporulation defective and fails to
CC perform premeiotic DNA synthesis. {ECO:0000269|PubMed:12586695}.
CC -!- MISCELLANEOUS: Present with 3400 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the fl(2)d family. {ECO:0000305}.
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DR EMBL; Z35926; CAA85000.1; -; Genomic_DNA.
DR EMBL; Z46260; CAA86401.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07176.1; -; Genomic_DNA.
DR PIR; S45915; S45915.
DR RefSeq; NP_009613.1; NM_001178405.1.
DR AlphaFoldDB; P38236; -.
DR SMR; P38236; -.
DR BioGRID; 32760; 328.
DR ComplexPortal; CPX-3211; MIS complex.
DR DIP; DIP-1299N; -.
DR IntAct; P38236; 18.
DR MINT; P38236; -.
DR STRING; 4932.YBR057C; -.
DR iPTMnet; P38236; -.
DR MaxQB; P38236; -.
DR PaxDb; P38236; -.
DR PRIDE; P38236; -.
DR EnsemblFungi; YBR057C_mRNA; YBR057C; YBR057C.
DR GeneID; 852348; -.
DR KEGG; sce:YBR057C; -.
DR SGD; S000000261; MUM2.
DR VEuPathDB; FungiDB:YBR057C; -.
DR eggNOG; ENOG502S20W; Eukaryota.
DR HOGENOM; CLU_067405_1_0_1; -.
DR InParanoid; P38236; -.
DR OMA; NANIWRD; -.
DR BioCyc; YEAST:G3O-29028-MON; -.
DR PRO; PR:P38236; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38236; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR GO; GO:0036396; C:RNA N6-methyladenosine methyltransferase complex; IDA:UniProtKB.
DR GO; GO:0051321; P:meiotic cell cycle; IMP:SGD.
DR GO; GO:0080009; P:mRNA methylation; IMP:SGD.
DR GO; GO:2000221; P:negative regulation of pseudohyphal growth; IMP:SGD.
DR GO; GO:0006279; P:premeiotic DNA replication; IMP:SGD.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Cytoplasm; Meiosis; Nucleus; Reference proteome; Sporulation.
FT CHAIN 1..366
FT /note="Protein MUM2"
FT /id="PRO_0000096647"
FT REGION 28..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 60..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 366 AA; 41433 MW; E57C853BFE8E5E83 CRC64;
MNYMAYDYDP QHSLETSFNN LAFHPHQQSQ QQALYESGER NDARPGLMNT LGQASKMNNS
MLPQGSSASP LTGQHSLNST TNFNMPPSMN TYNYQNVPQA SMRNTLNHNN IMNGATANDY
WLDPMNNMTN NKDTNGNPND SMSSMSNMTA KTSINSTAFK NSFVPFNHVT ALSMNNVNSN
EMNSNKDDRM EALEVELQIK ESQIESLENE IQRLKKIFNE GLNYKQNEHK YEKENCHIPQ
TFELPASLEV IFRKLSSSLH AKEKELAETK ENLESILTAL ALNPTNSVTK YGRYDAESIA
HKMVVRLENL TNENKEMAKM LAYGRSKETQ IELQLAKKEN LELREKIASL EAHLASKESS
KEDVAN
