MUNS_REOVD
ID MUNS_REOVD Reviewed; 721 AA.
AC P12419; A4ZY25; Q9PY81;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 22-JUL-2008, sequence version 2.
DT 07-OCT-2020, entry version 57.
DE RecName: Full=Protein mu-NS;
DE Short=MuNS;
GN Name=M3;
OS Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3).
OC Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC Reovirales; Reoviridae; Spinareovirinae; Orthoreovirus.
OX NCBI_TaxID=10886;
OH NCBI_TaxID=40674; Mammalia.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA], AND ALTERNATIVE INITIATION.
RX PubMed=2523177; DOI=10.1016/0042-6822(89)90154-2;
RA Wiener J.R., Bartlett J.A., Joklik W.K.;
RT "The sequences of reovirus serotype 3 genome segments M1 and M3 encoding
RT the minor protein mu 2 and the major nonstructural protein mu NS,
RT respectively.";
RL Virology 169:293-304(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA], AND ALTERNATIVE INITIATION.
RX PubMed=10544126; DOI=10.1006/viro.1999.9990;
RA McCutcheon A.M., Broering T.J., Nibert M.L.;
RT "Mammalian reovirus M3 gene sequences and conservation of coiled-coil
RT motifs near the carboxyl terminus of the microNS protein.";
RL Virology 264:16-24(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Infectious clone;
RX PubMed=18005692; DOI=10.1016/j.chom.2007.03.003;
RA Kobayashi T., Antar A.A., Boehme K.W., Danthi P., Eby E.A., Guglielmi K.M.,
RA Holm G.H., Johnson E.M., Maginnis M.S., Naik S., Skelton W.B., Wetzel J.D.,
RA Wilson G.J., Chappell J.D., Dermody T.S.;
RT "A plasmid-based reverse genetics system for animal double-stranded RNA
RT viruses.";
RL Cell Host Microbe 1:147-157(2007).
CC -!- FUNCTION: Non-structural protein with ssRNA-binding activity. Is
CC probably involved in the formation of viral inclusions, where the
CC assembly of cores and the replication of viral RNA are thought to
CC occur. Together with mu-2, recruits the other core proteins to these
CC inclusions (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with mu-2, sigma-NS, inner capsid proteins lambda-1
CC and sigma-2, and outer capsid protein lambda-2; in viral inclusions.
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=mu-NS;
CC IsoId=P12419-1; Sequence=Displayed;
CC Name=mu-NSC;
CC IsoId=P12419-2; Sequence=VSP_034862;
CC -!- PTM: The N-terminus is blocked.
CC -!- MISCELLANEOUS: [Isoform mu-NSC]: It is unsure whether Met-41 is the
CC initiator. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the orthoreovirus mu-NS protein family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M27262; AAA47260.1; -; Genomic_RNA.
DR EMBL; AF174384; AAF13171.1; -; Genomic_RNA.
DR EMBL; EF494440; ABP48918.1; -; Genomic_RNA.
DR PIR; B30179; MNXR3D.
DR SMR; P12419; -.
DR Proteomes; UP000006373; Genome.
DR Proteomes; UP000165799; Genome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
PE 3: Inferred from homology;
KW Alternative initiation; Coiled coil; Reference proteome; RNA-binding.
FT CHAIN 1..721
FT /note="Protein mu-NS"
FT /id="PRO_0000222748"
FT REGION 1..13
FT /note="Interaction with sigma-NS"
FT /evidence="ECO:0000250"
FT REGION 14..40
FT /note="Interaction with mu-2"
FT /evidence="ECO:0000250"
FT COILED 522..559
FT /evidence="ECO:0000255"
FT COILED 628..684
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..40
FT /note="Missing (in isoform mu-NSC)"
FT /evidence="ECO:0000305"
FT /id="VSP_034862"
FT CONFLICT 49
FT /note="T -> I (in Ref. 1; AAA47260)"
FT /evidence="ECO:0000305"
FT CONFLICT 127
FT /note="Missing (in Ref. 1; AAA47260)"
FT /evidence="ECO:0000305"
FT CONFLICT 180
FT /note="E -> K (in Ref. 3; ABP48918)"
FT /evidence="ECO:0000305"
FT CONFLICT 201..202
FT /note="LV -> QL (in Ref. 3; ABP48918)"
FT /evidence="ECO:0000305"
FT CONFLICT 201
FT /note="L -> H (in Ref. 1; AAA47260)"
FT /evidence="ECO:0000305"
FT CONFLICT 274
FT /note="D -> H (in Ref. 1; AAA47260)"
FT /evidence="ECO:0000305"
FT CONFLICT 302
FT /note="L -> R (in Ref. 1; AAA47260)"
FT /evidence="ECO:0000305"
FT CONFLICT 429
FT /note="T -> N (in Ref. 1; AAA47260)"
FT /evidence="ECO:0000305"
FT CONFLICT 587
FT /note="Missing (in Ref. 1; AAA47260)"
FT /evidence="ECO:0000305"
FT CONFLICT 705..707
FT /note="VDD -> ADG (in Ref. 3; ABP48918)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 721 AA; 80158 MW; 8E93017633E95940 CRC64;
MASFKGFSAN TVPVSKAKRD ISSLAATPGL RSQSFTPSVD MSQSREFLTK AIEQGSMSIP
YQHVNVPKVD RKVVSLVVRP FSSGAFSISG VISPAHAYLL ECLPQLEQAM AFVASPESFQ
ASDVAKRFAI KPGMSLQDAI TAFINFVSAM LKMTVTRQNF DVIVAEIERL ASTSVSVRTE
EAKVADEELM LFGLDHRGPQ LVDVSDAKGI MKAADIQTTH DVHLAPGVGN IDPEIYNEGR
FMFMQHKPLA ADQSYFTLET ADYFKIYPTY DEHDGRMADQ KQSGLILCTK DEVLAEQTIF
KLDAPDDKTV HLLDRDDDHV VARFTKVFIE DVAPGHHAAQ RSGQRSVLDD LYANTQVISI
TSAALKWVVK HGVSDGIVNR KNVKVCVGFD PLYTLSTHNG VSLCALLMDE KLSVLNSACR
MTLRSLMKTG RDVDAHRAFQ RVLSQGYTSL MCYYHPSRKL AYGEVLFLER SNDVTDGIKL
QLDASRQCHE CPVLQQKVVE LEKQIIMQKS IQSDPTPVAL QPLLSQLREL SSEVTRLQME
LSRAQSLNAQ LEADVKSAQS CSLDMYLRHH TCINGHAKED ELLDAVRVAP DVRREIMEKR
SEVRQGWCER ISKEAAAKCQ TVIDDLTLMN GKQAQEITEL RDSAEKYEKQ IAELVSTITQ
NQITYQQELQ ALVAKNVELD ALNQRQAKSL RITPSLLSAT PIDSVDDVAD LIDFSVPTDE
L
