ID   MUNS_REOVJ              Reviewed;         721 AA.
AC   Q9PY82;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=Protein mu-NS;
DE            Short=MuNS;
GN   Name=M3;
OS   Reovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Spinareovirinae; Orthoreovirus.
OX   NCBI_TaxID=10885;
OH   NCBI_TaxID=40674; Mammalia.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA], AND ALTERNATIVE INITIATION.
RX   PubMed=10544126; DOI=10.1006/viro.1999.9990;
RA   McCutcheon A.M., Broering T.J., Nibert M.L.;
RT   "Mammalian reovirus M3 gene sequences and conservation of coiled-coil
RT   motifs near the carboxyl terminus of the microNS protein.";
RL   Virology 264:16-24(1999).
CC   -!- FUNCTION: Non-structural protein with ssRNA-binding activity. Is
CC       probably involved in the formation of viral inclusions, where the
CC       assembly of cores and the replication of viral RNA are thought to
CC       occur. Together with mu-2, recruits the other core proteins to these
CC       inclusions (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with mu-2, sigma-NS, inner capsid proteins lambda-1
CC       and sigma-2, and outer capsid protein lambda-2; in viral inclusions.
CC       {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=mu-NS;
CC         IsoId=Q9PY82-1; Sequence=Displayed;
CC       Name=mu-NSC;
CC         IsoId=Q9PY82-2; Sequence=VSP_034869;
CC   -!- PTM: The N-terminus is blocked. {ECO:0000250}.
CC   -!- MISCELLANEOUS: [Isoform mu-NSC]: It is unsure whether Met-41 is the
CC       initiator. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the orthoreovirus mu-NS protein family.
CC       {ECO:0000305}.
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DR   EMBL; AF174383; AAF13170.1; -; mRNA.
DR   SMR; Q9PY82; -.
DR   PRIDE; Q9PY82; -.
DR   Proteomes; UP000006370; Genome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Alternative initiation; Coiled coil; RNA-binding.
FT   CHAIN           1..721
FT                   /note="Protein mu-NS"
FT                   /id="PRO_0000345002"
FT   REGION          1..13
FT                   /note="Interaction with sigma-NS"
FT                   /evidence="ECO:0000250"
FT   REGION          14..40
FT                   /note="Interaction with mu-2"
FT                   /evidence="ECO:0000250"
FT   REGION          17..37
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          523..556
FT                   /evidence="ECO:0000255"
FT   COILED          632..686
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         1..40
FT                   /note="Missing (in isoform mu-NSC)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_034869"
SQ   SEQUENCE   721 AA;  80505 MW;  0C08763E6E278153 CRC64;
     MASFKGFSAN TVPVSKTRKD TSSLTATPGL RAPSMSSPVD MAQSREFLTK AIEHGSMSIP
     YQHVNVPKVD RKVVSLVVRP FSAGAFSISG VISPAHAYLL ECLPQLEQAM AFVASPEAFQ
     ASDVAKRFTI KPGMSLQDAI TAFINFVSAM LKMTVTRQNF DVIIAEIERL ASSGVVNRTE
     EAKVADEELM LFGLDHRAPQ QIDVSEPVGI SRAVEIQTTN NVHLAPGLGN IDPEIYNEGR
     FMFMQHKPLA ADQSYFTTET ADYFKIYPTY DEHDGRMVDQ KQSGLILCTK DEVLAEQTIF
     KLDVPDDKTV HLLDRDDDHV VARFTRVFIE DVAPSHHAAQ RSNQRSLLDD LYANTQVVSV
     TPSALRWVIK HGVSDGIVNR KNVKICVGFD PLYTLATSNG LSLCSILMDE KLSVLNSACK
     MTLRSLLKTH RDLDLHRAFQ RVISQSYASL MCYYHPSRKL AYGELLFMSS QSDTVDGIKL
     QLDASRQCHE CPLLQQKIVE LEKHLIVQKS ASSDPTPVAL QPLLSQLREL SSEVTRLQMD
     LSRTQAINTR LEADVKSAQS CSLDMYLKHH TCINSHVKED ELMDAVRIAP DVRQELMLKR
     KATRQEWWER IARETSTTFQ SKIDELTLMN GKQAHEISEL RDSVTNYEKQ VAELVSTITQ
     NQTTYQQELQ ALVAKNIELD ALNQRQAKSV RITSSLLSAT PIDAVDGASD LIDFSVPADE
     L