ID   MUNS_REOVL              Reviewed;         721 AA.
AC   Q9PY83;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 43.
DE   RecName: Full=Protein mu-NS;
DE            Short=MuNS;
GN   Name=M3;
OS   Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Spinareovirinae; Orthoreovirus.
OX   NCBI_TaxID=10884;
OH   NCBI_TaxID=40674; Mammalia.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA], AND ALTERNATIVE INITIATION.
RX   PubMed=10544126; DOI=10.1006/viro.1999.9990;
RA   McCutcheon A.M., Broering T.J., Nibert M.L.;
RT   "Mammalian reovirus M3 gene sequences and conservation of coiled-coil
RT   motifs near the carboxyl terminus of the microNS protein.";
RL   Virology 264:16-24(1999).
RN   [2]
RP   INTERACTION WITH PROTEIN SIGMA-NS AND PROTEIN MU-2.
RX   PubMed=12663763; DOI=10.1128/jvi.77.8.4566-4576.2003;
RA   Miller C.L., Broering T.J., Parker J.S.L., Arnold M.M., Nibert M.L.;
RT   "Reovirus sigma NS protein localizes to inclusions through an association
RT   requiring the mu NS amino terminus.";
RL   J. Virol. 77:4566-4576(2003).
RN   [3]
RP   FUNCTION, AND INTERACTION WITH PROTEIN LAMBDA-1; PROTEIN LAMBDA-2 AND
RP   PROTEIN SIGMA-2.
RX   PubMed=14747553; DOI=10.1128/jvi.78.4.1882-1892.2004;
RA   Broering T.J., Kim J., Miller C.L., Piggott C.D., Dinoso J.B., Nibert M.L.,
RA   Parker J.S.L.;
RT   "Reovirus nonstructural protein mu NS recruits viral core surface proteins
RT   and entering core particles to factory-like inclusions.";
RL   J. Virol. 78:1882-1892(2004).
CC   -!- FUNCTION: Non-structural protein with ssRNA-binding activity. Is
CC       probably involved in the formation of viral inclusions, where the
CC       assembly of cores and the replication of viral RNA are thought to
CC       occur. Together with mu-2, recruits the other core proteins to these
CC       inclusions. {ECO:0000269|PubMed:14747553}.
CC   -!- SUBUNIT: Interacts with mu-2, sigma-NS, inner capsid proteins lambda-1
CC       and sigma-2, and outer capsid protein lambda-2; in viral inclusions.
CC       {ECO:0000269|PubMed:12663763, ECO:0000269|PubMed:14747553}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=mu-NS;
CC         IsoId=Q9PY83-1; Sequence=Displayed;
CC       Name=mu-NSC;
CC         IsoId=Q9PY83-2; Sequence=VSP_034870;
CC   -!- PTM: The N-terminus is blocked. {ECO:0000250}.
CC   -!- MISCELLANEOUS: [Isoform mu-NSC]: It is unsure whether Met-41 is the
CC       initiator. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the orthoreovirus mu-NS protein family.
CC       {ECO:0000305}.
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DR   EMBL; AF174382; AAF13169.1; -; mRNA.
DR   SMR; Q9PY83; -.
DR   PRIDE; Q9PY83; -.
DR   Proteomes; UP000007253; Genome.
DR   GO; GO:0039714; C:cytoplasmic viral factory; IDA:UniProtKB.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Alternative initiation; Coiled coil; Reference proteome; RNA-binding.
FT   CHAIN           1..721
FT                   /note="Protein mu-NS"
FT                   /id="PRO_0000345003"
FT   REGION          1..13
FT                   /note="Interaction with sigma-NS"
FT   REGION          14..40
FT                   /note="Interaction with mu-2"
FT   COILED          523..560
FT                   /evidence="ECO:0000255"
FT   COILED          628..686
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         1..40
FT                   /note="Missing (in isoform mu-NSC)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_034870"
SQ   SEQUENCE   721 AA;  80166 MW;  C84FED8A68ED53C9 CRC64;
     MASFKGFSVN TVPVSKAKRD ISSLAATPGI RSQPFTPSVD MSQSREFLTK AIEQGSMSIP
     YQHVNVPKVD RKVVSLVVRP FSSGAFSISG VISPAHAYLL DCLPQLEQAM AFVASPESFQ
     ASDVAKRFAI KPGMSLQDAI TAFINFVSAM LKMTVTRQNF DVIVAEIERL ASTSVSVRTE
     EAKVADEELM LFGLDHRGPQ QLDISNAKGI MKAADIQATH DVHLAPGVGN IDPEIYNEGR
     FMFMQHKPLA ADQSYFTLET ADYFKIYPTY DEHDSRMADQ KQSGLILCTK DEVLAEQTIF
     KLDAPDDKTV HLLDRDDDHV VARFTKVFIE DVAPGHHAAQ RSGQRSVLDD LYANTQVVSI
     TSAALKWVVK HGVSDGIVNR KNVKVCVGFD PLYTLSTHNG VSLCALLMDE KLSVLNSACR
     MTLRSLMKTG RDADAHRAFQ RVLSQGYASL MCYYHPSRKL AYGEVLFLER SSDMVDGIKL
     QLDASRQCHE CPVLQQKVVE LEKQIIMQKS IQSDPTPMAL QPLLSQLREL SSEVTRLQME
     LSRTQSLNAQ LEADAKSAQA CSLDMYLRHH TCINGHTKED ELLDAVRVAP DVRKEIMEKR
     GEVRRGWCER ISKEAAAKCQ TVIDDLTQMN GKQAREITEL RESAENYEKQ IAELVGTITQ
     NQMTYQQELQ ALVAKNVELD TMNQRQAKSL RITPSLLSAT PIDSVDGAAD LIDFSVPTDE
     L