MUP3_YEAST
ID MUP3_YEAST Reviewed; 546 AA.
AC P38734; D3DKT2;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Low-affinity methionine permease;
GN Name=MUP3; OrderedLocusNames=YHL036W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP CHARACTERIZATION.
RX PubMed=8893857; DOI=10.1006/jmbi.1996.0529;
RA Isnard A.D., Thomas D., Surdin-Kerjan Y.;
RT "The study of methionine uptake in Saccharomyces cerevisiae reveals a new
RT family of amino acid permeases.";
RL J. Mol. Biol. 262:473-484(1996).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Very low affinity permease for methionine.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: To yeast high affinity methionine permease (MUP1).
CC {ECO:0000305}.
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DR EMBL; U11583; AAB65048.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06649.1; -; Genomic_DNA.
DR PIR; S48932; S48932.
DR RefSeq; NP_011827.1; NM_001179116.1.
DR AlphaFoldDB; P38734; -.
DR SMR; P38734; -.
DR BioGRID; 36386; 54.
DR DIP; DIP-5572N; -.
DR IntAct; P38734; 1.
DR STRING; 4932.YHL036W; -.
DR TCDB; 2.A.3.8.16; the amino acid-polyamine-organocation (apc) family.
DR PaxDb; P38734; -.
DR PRIDE; P38734; -.
DR EnsemblFungi; YHL036W_mRNA; YHL036W; YHL036W.
DR GeneID; 856349; -.
DR KEGG; sce:YHL036W; -.
DR SGD; S000001028; MUP3.
DR VEuPathDB; FungiDB:YHL036W; -.
DR eggNOG; KOG1287; Eukaryota.
DR HOGENOM; CLU_013661_4_0_1; -.
DR InParanoid; P38734; -.
DR OMA; SPNVHYV; -.
DR BioCyc; YEAST:G3O-31055-MON; -.
DR Reactome; R-SCE-352230; Amino acid transport across the plasma membrane.
DR PRO; PR:P38734; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38734; protein.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015191; F:L-methionine transmembrane transporter activity; IMP:SGD.
DR GO; GO:1903692; P:methionine import across plasma membrane; IMP:SGD.
DR InterPro; IPR002293; AA/rel_permease1.
DR Pfam; PF13520; AA_permease_2; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..546
FT /note="Low-affinity methionine permease"
FT /id="PRO_0000096649"
FT TOPO_DOM 1..70
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 92..98
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..148
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 170..188
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 210..213
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 235..254
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 276..297
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 319
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 341..346
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 347..367
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 368..393
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 394..414
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 415..423
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 424..444
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 445..459
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 460..480
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 481..494
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 495..515
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 516..546
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 546 AA; 60620 MW; 9C9767A181FDDCAE CRC64;
MEPLLFNSGK ANPSQDVFID VEVGDITTKY GSTNTGSFSS MDTVEAQAIK AETARFMEVP
QGRHLGVFST VVLFVSRIMG SGIFAVPSVI LLNTGGNKLI YFAIWVFSAA IAFAGLYLFL
EFGSWIPKSG GRKNFLERSF ERPRLLISVV FSCYSVLTGY ALTGSIVFGK YVLSAFGVTD
DSWSKYVSIS FIIFAVLIHG VSVRHGVFIQ NALGGLKLIM IVLMCFAGLY TLFFYKSTGQ
VAWDLPVTQV EKDSLLSVSS IATAFISSFF CFSGWDTVHT VTSEIKNPVK TLKVSGPLSL
IICFVCYTMM NVAYLKVLTY EEIVSAGPLV GSVLFTKLFG PRVGGKFIAF SIAISAASNI
LVVIYSISRV NQEIFKEGYL PFSIHMSKNW PFDAPLPSIS LCGFITIAWI LILPKEGESF
NYLVSMDGYG NQFFLLLVAI GLFIWRFKHK NEVPEIRAST FGVLAIITLS LYMLMAPFFA
DPSLNRVGFL PPYQIMSLLV IVACFFFWLV KFVLLPKFFH YKLLPKITYL HDGLIVTEWV
KKPCLC
