MUPS1_ARATH
ID MUPS1_ARATH Reviewed; 377 AA.
AC F4KDF5; C9X3W2; Q9FJM6;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2016, sequence version 2.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=Protein MULTIPOLAR SPINDLE 1 {ECO:0000303|PubMed:19500302};
DE AltName: Full=Protein PUTATIVE RECOMBINATION INITIATION DEFECTS 2 {ECO:0000303|PubMed:19763177};
DE Short=AtPRD2 {ECO:0000303|PubMed:19763177};
GN Name=MPS1 {ECO:0000303|PubMed:19500302};
GN Synonyms=PRD2 {ECO:0000303|PubMed:19763177};
GN OrderedLocusNames=At5g57880 {ECO:0000312|Araport:AT5G57880};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia, and cv. Wassilewskija; TISSUE=Flower bud;
RX PubMed=19763177; DOI=10.1371/journal.pgen.1000654;
RA De Muyt A., Pereira L., Vezon D., Chelysheva L., Gendrot G., Chambon A.,
RA Laine-Choinard S., Pelletier G., Mercier R., Nogue F., Grelon M.;
RT "A high throughput genetic screen identifies new early meiotic
RT recombination functions in Arabidopsis thaliana.";
RL PLoS Genet. 5:E1000654-E1000654(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT features of the regions of 1,367,185 bp covered by 19 physically assigned
RT P1 and TAC clones.";
RL DNA Res. 5:203-216(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=19500302; DOI=10.1111/j.1365-313x.2009.03929.x;
RA Jiang H., Wang F.-F., Wu Y.-T., Zhou X., Huang X.-Y., Zhu J., Gao J.-F.,
RA Dong R.-B., Cao K.-M., Yang Z.-N.;
RT "MULTIPOLAR SPINDLE 1 (MPS1), a novel coiled-coil protein of Arabidopsis
RT thaliana, is required for meiotic spindle organization.";
RL Plant J. 59:1001-1010(2009).
CC -!- FUNCTION: Involved in meiotic spindle organization in meiocytes thus
CC regulating chromosome segregation (PubMed:19500302). Required for
CC formation of meiotic DNA double-strand breaks (DSBs) during early
CC recombination processes (PubMed:19763177).
CC {ECO:0000269|PubMed:19500302, ECO:0000269|PubMed:19763177}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768}.
CC Cytoplasm, cytoskeleton, spindle {ECO:0000305|PubMed:19500302}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, leaves, inflorescences
CC and seedlings. Strongly expressed in meiocytes.
CC {ECO:0000269|PubMed:19500302}.
CC -!- DEVELOPMENTAL STAGE: In anthers, predominantly detected in meiocytes
CC and tapetal cells from stage 5 to early stage 7, with highest levels at
CC stage 6, the time of male meiosis. In ovules, present in female
CC meiocytes and embryo sacs. {ECO:0000269|PubMed:19500302}.
CC -!- DISRUPTION PHENOTYPE: Defective in early recombination processes
CC leading to the absence of meiotic DNA double-strand break (DSB)
CC formation (PubMed:19763177). Reduced silique elongation associated with
CC fertility defects involving both male and female gametophyte abortion
CC due to aberrant meiotic products (PubMed:19763177, PubMed:19500302).
CC Produced multiple uneven spores aborted in later stages during anther
CC development, due to abnormal chromosome segregation and unequal bipolar
CC or multipolar spindles in meiocytes (PubMed:19500302).
CC {ECO:0000269|PubMed:19500302, ECO:0000269|PubMed:19763177}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08858.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; FN356233; CAX83745.1; -; mRNA.
DR EMBL; AB013396; BAB08858.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED96966.2; -; Genomic_DNA.
DR RefSeq; NP_001318822.1; NM_001345277.1.
DR AlphaFoldDB; F4KDF5; -.
DR SMR; F4KDF5; -.
DR STRING; 3702.AT5G57880.1; -.
DR PaxDb; F4KDF5; -.
DR PRIDE; F4KDF5; -.
DR ProteomicsDB; 238904; -.
DR EnsemblPlants; AT5G57880.1; AT5G57880.1; AT5G57880.
DR GeneID; 835898; -.
DR Gramene; AT5G57880.1; AT5G57880.1; AT5G57880.
DR KEGG; ath:AT5G57880; -.
DR Araport; AT5G57880; -.
DR TAIR; locus:2174363; AT5G57880.
DR eggNOG; ENOG502QVCM; Eukaryota.
DR HOGENOM; CLU_044141_0_0_1; -.
DR InParanoid; F4KDF5; -.
DR OMA; SNMFIMI; -.
DR OrthoDB; 965665at2759; -.
DR PRO; PR:F4KDF5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4KDF5; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0051026; P:chiasma assembly; IMP:TAIR.
DR GO; GO:0007059; P:chromosome segregation; IMP:TAIR.
DR GO; GO:0009553; P:embryo sac development; IMP:TAIR.
DR GO; GO:0007140; P:male meiotic nuclear division; IMP:TAIR.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IMP:TAIR.
DR GO; GO:0000212; P:meiotic spindle organization; IMP:TAIR.
DR GO; GO:0048236; P:plant-type sporogenesis; IMP:TAIR.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR InterPro; IPR037500; Msp1.
DR PANTHER; PTHR35768; PTHR35768; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Cytoskeleton; Developmental protein; DNA recombination; Meiosis;
KW Nucleus; Reference proteome.
FT CHAIN 1..377
FT /note="Protein MULTIPOLAR SPINDLE 1"
FT /evidence="ECO:0000255"
FT /id="PRO_0000438412"
FT MOTIF 117..124
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT CONFLICT 31
FT /note="Q -> H (in Ref. 1; CAX83745)"
FT /evidence="ECO:0000305"
FT CONFLICT 221
FT /note="V -> F (in Ref. 1; CAX83745)"
FT /evidence="ECO:0000305"
FT CONFLICT 305
FT /note="A -> AE (in Ref. 1; CAX83745)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 377 AA; 43661 MW; 6B50594A96D19E8D CRC64;
MSSSVAEANH TEKEESLRLA IAVSLLRSKF QNHQSSSSTS RCYVSSESDA LRWKQKAKER
KKEIIRLQED LKDAESSFHR DLFPANASCK CYFFDNLGVF SGRRIGEASE SRFNDVLRRR
FLRLARRRSR RKLTRSSQRL QPSEPDYEEE AEHLRISIDF LLELSEADSN DSNFSNWSHQ
AVDFIFASLK KLISMGRNLE SVEESISFMI TQLITRMCTP VKGNEVKQLE TSVGFYVQHL
IRKLGSEPFI GQRAIFAISQ RISILAENLL FMDPFDESFP EMDECMFILI QLIEFLICDY
LLPWANEAFD NVMFEEWIAS VVHARKAVKA LEERNGLYLL YMDRVTGELA KRVGQITSFR
EVEPAILDKI LAYQEIE
