MURC_YERPE
ID MURC_YERPE Reviewed; 491 AA.
AC Q8ZIE8; Q0WJB1;
DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=UDP-N-acetylmuramate--L-alanine ligase {ECO:0000255|HAMAP-Rule:MF_00046};
DE EC=6.3.2.8 {ECO:0000255|HAMAP-Rule:MF_00046};
DE AltName: Full=UDP-N-acetylmuramoyl-L-alanine synthetase {ECO:0000255|HAMAP-Rule:MF_00046};
GN Name=murC {ECO:0000255|HAMAP-Rule:MF_00046};
GN OrderedLocusNames=YPO0556, y3625, YP_3628;
OS Yersinia pestis.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=632;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CO-92 / Biovar Orientalis;
RX PubMed=11586360; DOI=10.1038/35097083;
RA Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA Stevens K., Whitehead S., Barrell B.G.;
RT "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL Nature 413:523-527(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KIM10+ / Biovar Mediaevalis;
RX PubMed=12142430; DOI=10.1128/jb.184.16.4601-4611.2002;
RA Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D.,
RA Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A.,
RA Nilles M.L., Matson J.S., Blattner F.R., Perry R.D.;
RT "Genome sequence of Yersinia pestis KIM.";
RL J. Bacteriol. 184:4601-4611(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=91001 / Biovar Mediaevalis;
RX PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA Wang J., Huang P., Yang R.;
RT "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT avirulent to humans.";
RL DNA Res. 11:179-197(2004).
CC -!- FUNCTION: Cell wall formation. {ECO:0000255|HAMAP-Rule:MF_00046}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-alanine + UDP-N-acetyl-alpha-D-muramate = ADP + H(+) +
CC phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine;
CC Xref=Rhea:RHEA:23372, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:57972, ChEBI:CHEBI:70757,
CC ChEBI:CHEBI:83898, ChEBI:CHEBI:456216; EC=6.3.2.8;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00046};
CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00046}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00046}.
CC -!- SIMILARITY: Belongs to the MurCDEF family. {ECO:0000255|HAMAP-
CC Rule:MF_00046}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM87173.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAS63776.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AL590842; CAL19235.1; -; Genomic_DNA.
DR EMBL; AE009952; AAM87173.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE017042; AAS63776.1; ALT_INIT; Genomic_DNA.
DR PIR; AI0068; AI0068.
DR RefSeq; WP_002216457.1; NZ_WHLN01000070.1.
DR RefSeq; YP_002345627.1; NC_003143.1.
DR PDB; 4HV4; X-ray; 2.25 A; A/B=1-491.
DR PDBsum; 4HV4; -.
DR AlphaFoldDB; Q8ZIE8; -.
DR SMR; Q8ZIE8; -.
DR IntAct; Q8ZIE8; 1.
DR STRING; 214092.YPO0556; -.
DR PaxDb; Q8ZIE8; -.
DR DNASU; 1148572; -.
DR EnsemblBacteria; AAM87173; AAM87173; y3625.
DR EnsemblBacteria; AAS63776; AAS63776; YP_3628.
DR GeneID; 66842890; -.
DR KEGG; ype:YPO0556; -.
DR KEGG; ypk:y3625; -.
DR KEGG; ypm:YP_3628; -.
DR PATRIC; fig|214092.21.peg.809; -.
DR eggNOG; COG0773; Bacteria.
DR HOGENOM; CLU_028104_2_2_6; -.
DR OMA; DITYQLR; -.
DR UniPathway; UPA00219; -.
DR Proteomes; UP000000815; Chromosome.
DR Proteomes; UP000001019; Chromosome.
DR Proteomes; UP000002490; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008763; F:UDP-N-acetylmuramate-L-alanine ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR Gene3D; 3.40.1190.10; -; 1.
DR Gene3D; 3.90.190.20; -; 1.
DR HAMAP; MF_00046; MurC; 1.
DR InterPro; IPR036565; Mur-like_cat_sf.
DR InterPro; IPR004101; Mur_ligase_C.
DR InterPro; IPR036615; Mur_ligase_C_dom_sf.
DR InterPro; IPR013221; Mur_ligase_cen.
DR InterPro; IPR000713; Mur_ligase_N.
DR InterPro; IPR005758; UDP-N-AcMur_Ala_ligase_MurC.
DR Pfam; PF01225; Mur_ligase; 1.
DR Pfam; PF02875; Mur_ligase_C; 1.
DR Pfam; PF08245; Mur_ligase_M; 1.
DR SUPFAM; SSF53244; SSF53244; 1.
DR SUPFAM; SSF53623; SSF53623; 1.
DR TIGRFAMs; TIGR01082; murC; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cell cycle; Cell division; Cell shape;
KW Cell wall biogenesis/degradation; Cytoplasm; Ligase; Nucleotide-binding;
KW Peptidoglycan synthesis; Reference proteome.
FT CHAIN 1..491
FT /note="UDP-N-acetylmuramate--L-alanine ligase"
FT /id="PRO_0000182190"
FT BINDING 126..132
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00046"
FT HELIX 16..18
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 21..25
FT /evidence="ECO:0007829|PDB:4HV4"
FT TURN 26..28
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 32..41
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 45..49
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 55..62
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 66..70
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 73..76
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 80..84
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 93..100
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 105..107
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 108..116
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 119..125
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 127..129
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 130..143
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 149..156
FT /evidence="ECO:0007829|PDB:4HV4"
FT TURN 157..160
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 161..164
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 167..174
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 178..184
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 188..192
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 206..218
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 226..230
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 234..239
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 240..242
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 247..253
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 257..266
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 269..275
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 282..288
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 291..307
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 311..320
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 327..336
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 337..340
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 343..351
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 356..369
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 373..379
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 384..389
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 391..398
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 401..407
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 422..430
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 438..440
FT /evidence="ECO:0007829|PDB:4HV4"
FT TURN 443..445
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 446..453
FT /evidence="ECO:0007829|PDB:4HV4"
FT STRAND 458..463
FT /evidence="ECO:0007829|PDB:4HV4"
FT HELIX 468..477
FT /evidence="ECO:0007829|PDB:4HV4"
FT TURN 478..480
FT /evidence="ECO:0007829|PDB:4HV4"
SQ SEQUENCE 491 AA; 53654 MW; 852C270C93370759 CRC64;
MNTQQLAKLR TIVPEMRRVR HIHFVGIGGA GMGGIAEVLA NEGYQISGSD LAPNSVTQHL
TALGAQIYFH HRPENVLDAS VVVVSTAISA DNPEIVAARE ARIPVIRRAE MLAELMRYRH
GIAVAGTHGK TTTTAMLSSI YAEAGLDPTF VNGGLVKAAG THARLGSSRY LIAEADESDA
SFLHLQPMVA IVTNIEADHM DTYQGDFENL KQTFINFLHN LPFYGRAVMC IDDPVVRELL
PRVGRHITTY GFSDDADVQI ASYRQEGPQG HFTLRRQDKP LIEVTLNAPG RHNALNAAAA
VAVATEEGIE DEDILRALVG FQGTGRRFDF LGNFPLAPVN GKEGSAMLVD DYGHHPTEVD
ATIKAARAGW PDKRIVMLFQ PHRYTRTRDL YDDFANVLSQ VDVLLMLDVY AAGEPPIPGA
DSRALCRTIR NRGKLDPILV PDSESAPEML AQILNGEDLI LVQGAGNIGK IARKLAEHKL
QPQLKDEEHH G
