MURC_ZYMMO
ID MURC_ZYMMO Reviewed; 476 AA.
AC Q9RNM7; Q5NPA4;
DT 01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=UDP-N-acetylmuramate--L-alanine ligase {ECO:0000255|HAMAP-Rule:MF_00046};
DE EC=6.3.2.8 {ECO:0000255|HAMAP-Rule:MF_00046};
DE AltName: Full=UDP-N-acetylmuramoyl-L-alanine synthetase {ECO:0000255|HAMAP-Rule:MF_00046};
GN Name=murC {ECO:0000255|HAMAP-Rule:MF_00046}; OrderedLocusNames=ZMO0832;
OS Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC Zymomonadaceae; Zymomonas.
OX NCBI_TaxID=264203;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 31821 / ZM4 / CP4;
RA Um H.W., Kang H.S.;
RL Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 31821 / ZM4 / CP4;
RX PubMed=15592456; DOI=10.1038/nbt1045;
RA Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H.,
RA Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J.,
RA Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y., Kang H.L., Lee S.Y., Lee K.J.,
RA Kang H.S.;
RT "The genome sequence of the ethanologenic bacterium Zymomonas mobilis
RT ZM4.";
RL Nat. Biotechnol. 23:63-68(2005).
CC -!- FUNCTION: Cell wall formation. {ECO:0000255|HAMAP-Rule:MF_00046}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-alanine + UDP-N-acetyl-alpha-D-muramate = ADP + H(+) +
CC phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine;
CC Xref=Rhea:RHEA:23372, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:57972, ChEBI:CHEBI:70757,
CC ChEBI:CHEBI:83898, ChEBI:CHEBI:456216; EC=6.3.2.8;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00046};
CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00046}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00046}.
CC -!- SIMILARITY: Belongs to the MurCDEF family. {ECO:0000255|HAMAP-
CC Rule:MF_00046}.
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DR EMBL; AF179611; AAD53935.1; -; Genomic_DNA.
DR EMBL; AE008692; AAV89456.1; -; Genomic_DNA.
DR RefSeq; WP_011240702.1; NZ_CP035711.1.
DR AlphaFoldDB; Q9RNM7; -.
DR SMR; Q9RNM7; -.
DR STRING; 264203.ZMO0832; -.
DR EnsemblBacteria; AAV89456; AAV89456; ZMO0832.
DR GeneID; 58026640; -.
DR KEGG; zmo:ZMO0832; -.
DR eggNOG; COG0773; Bacteria.
DR HOGENOM; CLU_028104_2_2_5; -.
DR OMA; DITYQLR; -.
DR OrthoDB; 307881at2; -.
DR UniPathway; UPA00219; -.
DR Proteomes; UP000001173; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008763; F:UDP-N-acetylmuramate-L-alanine ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR Gene3D; 3.40.1190.10; -; 1.
DR Gene3D; 3.90.190.20; -; 1.
DR HAMAP; MF_00046; MurC; 1.
DR InterPro; IPR036565; Mur-like_cat_sf.
DR InterPro; IPR004101; Mur_ligase_C.
DR InterPro; IPR036615; Mur_ligase_C_dom_sf.
DR InterPro; IPR013221; Mur_ligase_cen.
DR InterPro; IPR000713; Mur_ligase_N.
DR InterPro; IPR005758; UDP-N-AcMur_Ala_ligase_MurC.
DR Pfam; PF01225; Mur_ligase; 1.
DR Pfam; PF02875; Mur_ligase_C; 1.
DR Pfam; PF08245; Mur_ligase_M; 1.
DR SUPFAM; SSF53244; SSF53244; 1.
DR SUPFAM; SSF53623; SSF53623; 1.
DR TIGRFAMs; TIGR01082; murC; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell cycle; Cell division; Cell shape;
KW Cell wall biogenesis/degradation; Cytoplasm; Ligase; Nucleotide-binding;
KW Peptidoglycan synthesis; Reference proteome.
FT CHAIN 1..476
FT /note="UDP-N-acetylmuramate--L-alanine ligase"
FT /id="PRO_0000182192"
FT BINDING 115..121
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00046"
FT CONFLICT 331
FT /note="G -> C (in Ref. 1; AAD53935)"
FT /evidence="ECO:0000305"
FT CONFLICT 373
FT /note="S -> L (in Ref. 1; AAD53935)"
FT /evidence="ECO:0000305"
FT CONFLICT 451
FT /note="L -> V (in Ref. 1; AAD53935)"
FT /evidence="ECO:0000305"
FT CONFLICT 462..476
FT /note="GLAADITSIREVHHL -> VLRQILPQSERFITYDDCYIFQYLK (in
FT Ref. 1; AAD53935)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 476 AA; 51023 MW; 29112C60EF8F69B0 CRC64;
MKGVGTDIGR IHFVGIGGIG MSGIAEVMHN LGYQVQGSDI SEGYTVDALR QMGIKVLIGH
HAENVKDAAV VVVSSAIHRG NPEVEAALEN RIPVVRRAEM LAELMRLKST VAVAGTHGKT
TTTSMVAALL DAGGIDPTVI NGGIINSYGS NARLGDSDWM VVEADESDGS FLRLDGTLAI
VTNIDPEHLD HYGSFEKVQD AFVEFVSNVP FYGAAFLCID HPVVQSILPR IRDRRLITYG
FSAQADIRAV DITPISGGNR FTAVIRGRDG DIRRIENIFL PMPGRHNIQN ALSAIGVALE
FSIPDAAIRD GFARFGGVKR RFSRVGEIKI GDGSVLVIDD YGHHPVEIKA VLSAARESAQ
QRVIAVVQPH RFSRLHDLMT EFQSAFNDAD MVFVAPVYAA GEQPIAGVDS QALVSGLKQH
GHRSAQAVDS PRSLAIALAD IIKADDIIIC LGAGDITKWA AGLAADITSI REVHHL
