ID   A115_TOBAC              Reviewed;         307 AA.
AC   P40691;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Auxin-induced protein PCNT115;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. White Burley;
RA   van der Zaal E.J.;
RL   Submitted (AUG-1990) to the EMBL/GenBank/DDBJ databases.
CC   -!- INDUCTION: By auxin.
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family. Aldo/keto
CC       reductase 2 subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X56267; CAA39708.1; -; mRNA.
DR   PIR; S16390; S16390.
DR   AlphaFoldDB; P40691; -.
DR   SMR; P40691; -.
DR   PRIDE; P40691; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004033; F:aldo-keto reductase (NADP) activity; IBA:GO_Central.
DR   GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.100; -; 1.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   SUPFAM; SSF51430; SSF51430; 1.
PE   2: Evidence at transcript level;
KW   Auxin signaling pathway; NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..307
FT                   /note="Auxin-induced protein PCNT115"
FT                   /id="PRO_0000070380"
FT   ACT_SITE        64
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         136
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         215..225
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   SITE            90
FT                   /note="Lowers pKa of active site Tyr"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   307 AA;  33857 MW;  C4417DA852613360 CRC64;
     MAKEGTKVPR IKLGSQGLEV SAQGLGCMGM SAFYGPPKPE PDMIQLIHHA INSGITLLDT
     SDVYGPHTNE ILLGKALKGG TRERVVLATK FGIVLGDEKK AEGKRAVHGD PAYVRAACEA
     SLKRLDIDCI DLYYQHRVDT RVPIEITVGE LKKLVEEGKL KYIGLSEASA STIRRAHAVH
     PITAVQLEWS LWSRDVEEEI IPTCRELGIG IVAYSPLGRG FLSSGPKLLE DMSNEDYRKY
     LPRFQAENLE NNKNLYERIC EMAVRKGCTP SQLALAWVHH QGNDVCPIPG TTKIENLNQN
     MKPCPSS