MURI_MYCS2
ID MURI_MYCS2 Reviewed; 277 AA.
AC A0R1X0; I7GEC4;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Glutamate racemase {ECO:0000255|HAMAP-Rule:MF_00258};
DE EC=5.1.1.3 {ECO:0000255|HAMAP-Rule:MF_00258};
GN Name=murI {ECO:0000255|HAMAP-Rule:MF_00258};
GN OrderedLocusNames=MSMEG_4903, MSMEI_4776;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
CC -!- FUNCTION: Provides the (R)-glutamate required for cell wall
CC biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00258}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-glutamate = D-glutamate; Xref=Rhea:RHEA:12813,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:29986; EC=5.1.1.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00258};
CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00258}.
CC -!- SIMILARITY: Belongs to the aspartate/glutamate racemases family.
CC {ECO:0000255|HAMAP-Rule:MF_00258}.
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DR EMBL; CP000480; ABK72762.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP41224.1; -; Genomic_DNA.
DR RefSeq; WP_011730179.1; NZ_SIJM01000024.1.
DR RefSeq; YP_889158.1; NC_008596.1.
DR PDB; 5IJW; X-ray; 1.76 A; A/B=1-277.
DR PDBsum; 5IJW; -.
DR AlphaFoldDB; A0R1X0; -.
DR SMR; A0R1X0; -.
DR STRING; 246196.MSMEI_4776; -.
DR EnsemblBacteria; ABK72762; ABK72762; MSMEG_4903.
DR EnsemblBacteria; AFP41224; AFP41224; MSMEI_4776.
DR GeneID; 66736207; -.
DR KEGG; msg:MSMEI_4776; -.
DR KEGG; msm:MSMEG_4903; -.
DR PATRIC; fig|246196.19.peg.4784; -.
DR eggNOG; COG0796; Bacteria.
DR OMA; MPWGPRT; -.
DR OrthoDB; 1718671at2; -.
DR BRENDA; 5.1.1.3; 3512.
DR UniPathway; UPA00219; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0008881; F:glutamate racemase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR HAMAP; MF_00258; Glu_racemase; 1.
DR InterPro; IPR015942; Asp/Glu/hydantoin_racemase.
DR InterPro; IPR001920; Asp/Glu_race.
DR InterPro; IPR018187; Asp/Glu_racemase_AS_1.
DR InterPro; IPR033134; Asp/Glu_racemase_AS_2.
DR InterPro; IPR004391; Glu_race.
DR PANTHER; PTHR21198:SF2; PTHR21198:SF2; 1.
DR Pfam; PF01177; Asp_Glu_race; 1.
DR SUPFAM; SSF53681; SSF53681; 2.
DR TIGRFAMs; TIGR00067; glut_race; 1.
DR PROSITE; PS00923; ASP_GLU_RACEMASE_1; 1.
DR PROSITE; PS00924; ASP_GLU_RACEMASE_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell shape; Cell wall biogenesis/degradation; Isomerase;
KW Peptidoglycan synthesis; Reference proteome.
FT CHAIN 1..277
FT /note="Glutamate racemase"
FT /id="PRO_1000047586"
FT ACT_SITE 75
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00258"
FT ACT_SITE 186
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00258"
FT BINDING 12..13
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00258"
FT BINDING 44..45
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00258"
FT BINDING 76..77
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00258"
FT BINDING 187..188
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00258"
FT STRAND 8..15
FT /evidence="ECO:0007829|PDB:5IJW"
FT HELIX 18..27
FT /evidence="ECO:0007829|PDB:5IJW"
FT STRAND 33..37
FT /evidence="ECO:0007829|PDB:5IJW"
FT TURN 39..41
FT /evidence="ECO:0007829|PDB:5IJW"
FT HELIX 49..65
FT /evidence="ECO:0007829|PDB:5IJW"
FT STRAND 69..73
FT /evidence="ECO:0007829|PDB:5IJW"
FT HELIX 76..82
FT /evidence="ECO:0007829|PDB:5IJW"
FT TURN 83..85
FT /evidence="ECO:0007829|PDB:5IJW"
FT HELIX 86..90
FT /evidence="ECO:0007829|PDB:5IJW"
FT STRAND 95..99
FT /evidence="ECO:0007829|PDB:5IJW"
FT HELIX 100..109
FT /evidence="ECO:0007829|PDB:5IJW"
FT STRAND 111..119
FT /evidence="ECO:0007829|PDB:5IJW"
FT HELIX 121..125
FT /evidence="ECO:0007829|PDB:5IJW"
FT HELIX 128..132
FT /evidence="ECO:0007829|PDB:5IJW"
FT HELIX 133..135
FT /evidence="ECO:0007829|PDB:5IJW"
FT STRAND 140..145
FT /evidence="ECO:0007829|PDB:5IJW"
FT HELIX 149..154
FT /evidence="ECO:0007829|PDB:5IJW"
FT HELIX 161..176
FT /evidence="ECO:0007829|PDB:5IJW"
FT STRAND 180..186
FT /evidence="ECO:0007829|PDB:5IJW"
FT HELIX 189..192
FT /evidence="ECO:0007829|PDB:5IJW"
FT HELIX 193..200
FT /evidence="ECO:0007829|PDB:5IJW"
FT STRAND 205..208
FT /evidence="ECO:0007829|PDB:5IJW"
FT HELIX 209..223
FT /evidence="ECO:0007829|PDB:5IJW"
FT STRAND 240..246
FT /evidence="ECO:0007829|PDB:5IJW"
FT HELIX 248..259
FT /evidence="ECO:0007829|PDB:5IJW"
FT TURN 263..265
FT /evidence="ECO:0007829|PDB:5IJW"
SQ SEQUENCE 277 AA; 29283 MW; 6E4BD345B82C8F62 CRC64;
MSDRLAPIGI FDSGVGGLTV ARAIIDQLPD EDIVYVGDTG NGPYGPLTIP QIRAHSLAIG
DDLVSRGVKA LVIACNTASS ACLRDARERY SPVPVVEVIL PAVRRAVAAT RNGRIGVIGT
QATIASGAYQ DAFAAARDTE VFTVACPRFV DFVERGVTSG RQVLGLAEGY LEPLQLAEVD
TLVLGCTHYP MLSGLIQLAM GDNVTLVSSA EETAKDLLRV LTELDLLRPH PDDPSVTAVR
RFEATGDPEA FTALAARFLG PTLDGVRPVR RHAGAGR
