ID   A12_VACCA               Reviewed;         187 AA.
AC   O57224;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   23-FEB-2022, entry version 52.
DE   RecName: Full=25 kDa core protein A12L;
DE   Contains:
DE     RecName: Full=17 kDa core protein A12L;
DE              Short=17K;
GN   OrderedLocusNames=MVA123L, ACAM3000_MVA_123; ORFNames=A12L;
OS   Vaccinia virus (strain Ankara) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=126794;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9601507; DOI=10.1006/viro.1998.9123;
RA   Antoine G., Scheiflinger F., Dorner F., Falkner F.G.;
RT   "The complete genomic sequence of the modified vaccinia Ankara strain:
RT   comparison with other orthopoxviruses.";
RL   Virology 244:365-396(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate Acambis 3000;
RA   Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J.,
RA   Khristova M., Wohlhueter R.M.;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the virion core that undergoes proteolytic
CC       processing during the immature virion (IV) to mature virion (MV)
CC       transition. Essential for the formation of a structurally normal core
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [25 kDa core protein A12L]: Virion {ECO:0000250}.
CC       Note=Localizes to the virion core. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [17 kDa core protein A12L]: Virion {ECO:0000250}.
CC       Note=Localizes to the virion core. {ECO:0000250}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC   -!- PTM: The 25-kDa precursor is cleaved to a mature protein of 17 kDa
CC       during virion maturation. Further proteolytic processing is supposed to
CC       occur since five more A12L-derived products have been observed (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae A12 family. {ECO:0000305}.
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DR   EMBL; U94848; AAB96463.1; -; Genomic_DNA.
DR   EMBL; AY603355; AAT10521.1; -; Genomic_DNA.
DR   PIR; T37399; T37399.
DR   SMR; O57224; -.
DR   PRIDE; O57224; -.
DR   Proteomes; UP000159908; Genome.
DR   Proteomes; UP000172909; Genome.
DR   InterPro; IPR006744; Poxvirus_A12.
DR   Pfam; PF04651; Pox_A12; 1.
PE   2: Evidence at transcript level;
KW   Late protein; Virion.
FT   CHAIN           1..187
FT                   /note="25 kDa core protein A12L"
FT                   /id="PRO_0000099235"
FT   CHAIN           1..56
FT                   /note="17 kDa core protein A12L"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000413913"
FT   REGION          66..99
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          147..173
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        159..173
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            56..57
FT                   /note="Cleavage; by I7 protease"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   187 AA;  20002 MW;  51050C749A397465 CRC64;
     MADKKNLAVR SSYDDYIETV NKITPQLKNL LAQIGGDAAV KGGNNNLNSQ TDVTAGACDT
     KCITCKPKSK SSSSSTSTSK GSKNTSGAPR RRTTVTTTSY NAMDGQIVQA VTNAGKIVYG
     TVRDGQLEVR GMVGEINHDL LGIDSVNAGK KKPSKKMPTN KKINMSSGMR RQEQINPDDC
     CLDMGMY