ID   A12_VACCC               Reviewed;         192 AA.
AC   P20989;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   23-FEB-2022, entry version 67.
DE   RecName: Full=25 kDa core protein A12L;
DE   Contains:
DE     RecName: Full=17 kDa core protein A12L;
DE              Short=17K;
GN   ORFNames=A12L;
OS   Vaccinia virus (strain Copenhagen) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10249;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "The complete DNA sequence of vaccinia virus.";
RL   Virology 179:247-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "Appendix to 'The complete DNA sequence of vaccinia virus'.";
RL   Virology 179:517-563(1990).
CC   -!- FUNCTION: Component of the virion core that undergoes proteolytic
CC       processing during the immature virion (IV) to mature virion (MV)
CC       transition. Essential for the formation of a structurally normal core
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [25 kDa core protein A12L]: Virion {ECO:0000250}.
CC       Note=Localizes to the virion core. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [17 kDa core protein A12L]: Virion {ECO:0000250}.
CC       Note=Localizes to the virion core. {ECO:0000250}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC   -!- PTM: The 25-kDa precursor is cleaved to a mature protein of 17 kDa
CC       during virion maturation. Further proteolytic processing is supposed to
CC       occur since five more A12L-derived products have been observed (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae A12 family. {ECO:0000305}.
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DR   EMBL; M35027; AAA48134.1; -; Genomic_DNA.
DR   PIR; E42518; E42518.
DR   SMR; P20989; -.
DR   DIP; DIP-2185N; -.
DR   IntAct; P20989; 1.
DR   MINT; P20989; -.
DR   Proteomes; UP000008269; Genome.
DR   InterPro; IPR006744; Poxvirus_A12.
DR   Pfam; PF04651; Pox_A12; 1.
PE   2: Evidence at transcript level;
KW   Late protein; Reference proteome; Virion.
FT   CHAIN           1..192
FT                   /note="25 kDa core protein A12L"
FT                   /id="PRO_0000099236"
FT   CHAIN           1..56
FT                   /note="17 kDa core protein A12L"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000413914"
FT   REGION          69..105
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          152..178
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        164..178
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            56..57
FT                   /note="Cleavage; by I7 protease"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   192 AA;  20519 MW;  C5966FD4B1217A86 CRC64;
     MADKKNLAVR SSYDDYIETV NKITPQLKNL LAQIGGDAAV KGGNNNLNSQ TDVTAGACDT
     KSKSSKCITC KPKSKSSSSS TSTSKGSKNT SGAPRRRTTV TTTSYNAMDG QIVQAVTNAG
     KIVYGTVRDG QLEVRGMVGE INHDLLGIDS VNAGKKKPSK KMPTNKKINM SSGMRRQEQI
     NPDDCCLDMG MY