MURJ_BURCJ
ID MURJ_BURCJ Reviewed; 516 AA.
AC B4E9G3;
DT 18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=Lipid II flippase MurJ {ECO:0000305};
GN Name=murJ {ECO:0000255|HAMAP-Rule:MF_02078, ECO:0000303|PubMed:24688094};
GN Synonyms=mviN {ECO:0000303|PubMed:24688094};
GN ORFNames=BCAL2764 {ECO:0000312|EMBL:CAR53064.1};
OS Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 /
OS NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315)).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX NCBI_TaxID=216591;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610;
RX PubMed=18931103; DOI=10.1128/jb.01230-08;
RA Holden M.T., Seth-Smith H.M., Crossman L.C., Sebaihia M., Bentley S.D.,
RA Cerdeno-Tarraga A.M., Thomson N.R., Bason N., Quail M.A., Sharp S.,
RA Cherevach I., Churcher C., Goodhead I., Hauser H., Holroyd N., Mungall K.,
RA Scott P., Walker D., White B., Rose H., Iversen P., Mil-Homens D.,
RA Rocha E.P., Fialho A.M., Baldwin A., Dowson C., Barrell B.G., Govan J.R.,
RA Vandamme P., Hart C.A., Mahenthiralingam E., Parkhill J.;
RT "The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of
RT cystic fibrosis patients.";
RL J. Bacteriol. 191:261-277(2009).
RN [2]
RP FUNCTION, PATHWAY, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF ARG-18; ARG-24;
RP ASP-39; ARG-52 AND ARG-274.
RX PubMed=24688094; DOI=10.1093/glycob/cwu025;
RA Mohamed Y.F., Valvano M.A.;
RT "A Burkholderia cenocepacia MurJ (MviN) homolog is essential for cell wall
RT peptidoglycan synthesis and bacterial viability.";
RL Glycobiology 24:564-576(2014).
CC -!- FUNCTION: Involved in peptidoglycan biosynthesis. Transports lipid-
CC linked peptidoglycan precursors from the inner to the outer leaflet of
CC the cytoplasmic membrane. {ECO:0000255|HAMAP-Rule:MF_02078,
CC ECO:0000305|PubMed:24688094}.
CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_02078, ECO:0000269|PubMed:24688094}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_02078}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_02078}.
CC -!- DISRUPTION PHENOTYPE: Essential for growth, it cannot be deleted. Under
CC non-permissive conditions, conditional mutant shows growth arrest,
CC dramatic morphological changes and cell lysis. Depletion causes
CC accumulation of peptidoglycan precursors in the cytoplasm.
CC {ECO:0000269|PubMed:24688094}.
CC -!- SIMILARITY: Belongs to the MurJ/MviN family. {ECO:0000255|HAMAP-
CC Rule:MF_02078, ECO:0000305}.
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DR EMBL; AM747720; CAR53064.1; -; Genomic_DNA.
DR RefSeq; WP_006482209.1; NC_011000.1.
DR AlphaFoldDB; B4E9G3; -.
DR SMR; B4E9G3; -.
DR STRING; 216591.BCAL2764; -.
DR EnsemblBacteria; CAR53064; CAR53064; BCAL2764.
DR GeneID; 56559136; -.
DR KEGG; bcj:BCAL2764; -.
DR eggNOG; COG0728; Bacteria.
DR HOGENOM; CLU_006797_5_3_4; -.
DR OMA; IFFVAFK; -.
DR OrthoDB; 749401at2; -.
DR BioCyc; BCEN216591:G1G1V-3062-MON; -.
DR UniPathway; UPA00219; -.
DR Proteomes; UP000001035; Chromosome 1.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0015648; F:lipid-linked peptidoglycan transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR CDD; cd13123; MATE_MurJ_like; 1.
DR HAMAP; MF_02078; MurJ_MviN; 1.
DR InterPro; IPR004268; MurJ.
DR Pfam; PF03023; MurJ; 1.
DR PIRSF; PIRSF002869; MviN; 1.
DR PRINTS; PR01806; VIRFACTRMVIN.
DR TIGRFAMs; TIGR01695; murJ_mviN; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Cell shape;
KW Cell wall biogenesis/degradation; Membrane; Peptidoglycan synthesis;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..516
FT /note="Lipid II flippase MurJ"
FT /id="PRO_0000438821"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 275..295
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 358..378
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 390..409
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 448..468
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 483..503
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT MUTAGEN 18
FT /note="R->A: Lack of activity."
FT /evidence="ECO:0000269|PubMed:24688094"
FT MUTAGEN 24
FT /note="R->A: Lack of activity."
FT /evidence="ECO:0000269|PubMed:24688094"
FT MUTAGEN 39
FT /note="D->A: No change in activity."
FT /evidence="ECO:0000269|PubMed:24688094"
FT MUTAGEN 52
FT /note="R->A: No change in activity."
FT /evidence="ECO:0000269|PubMed:24688094"
FT MUTAGEN 274
FT /note="R->A: Lack of activity."
FT /evidence="ECO:0000269|PubMed:24688094"
SQ SEQUENCE 516 AA; 55652 MW; 8820621EFD3FAAEF CRC64;
MNLFRALLTV SGFTLLSRVT GLARETLIAR AFGASQYTDA FYVAFRIPNL LRRLSAEGAF
SQAFVPILAE FKNQQGHDAT KALVDAMSTV LAWALAVLSV VGIAGASWVV FAVASGLHSD
GQAFPLAVTM TRIMFPYIVF ISLTTLASGV LNTYKSFSLP AFAPVLLNVA FIAAAVFVAP
HLKVPVYALA WAVIVGGVLQ FLVQLPGLKK VDMVPLIGLN PLRALRHPGV KRVLAKMVPA
TFAVSVAQLS LIINTNIASR LGQGAVSWIN YADRLMEFPT ALLGVALGTI LLPSLSKAHV
DADSHEYSAL LDWGLRVTFL LAAPSALALF FFATPLTATL FNYGKFDAHT VTMVARALAT
YGIGLVGIIL IKILAPGFYA KQDIKTPVKI AIGVLIVTQL SNYVFVPLIG HAGLTLSIGV
GACLNSLLLF LGLRKRGIYQ PSPGWLRFFV QLVGAALVLA GLMHWCAINF DWTGMRAQPL
DRIALMAACL VLFAALYFGM LWVMGFKYAY FRRRAK
