MURJ_SALTY
ID MURJ_SALTY Reviewed; 524 AA.
AC P37169;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Probable lipid II flippase MurJ {ECO:0000255|HAMAP-Rule:MF_02078};
GN Name=murJ {ECO:0000255|HAMAP-Rule:MF_02078}; Synonyms=mviB, mviN, mviS;
GN OrderedLocusNames=STM1170;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2 / KK1004;
RA van Slooten J.-C., Okada T., Kutsukake K., Pechere J.-C., Harayama S.;
RL Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RX PubMed=8200538; DOI=10.1016/0378-1119(94)90603-3;
RA Kutsukake K., Okada T., Yokoseki T., Iino T.;
RT "Sequence analysis of the flgA gene and its adjacent region in Salmonella
RT typhimurium, and identification of another flagellar gene, flgN.";
RL Gene 143:49-54(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [4]
RP PROPOSED FUNCTION IN MOUSE VIRULENCE.
RC STRAIN=LT2 / SL488;
RX PubMed=2680969; DOI=10.1128/iai.57.11.3276-3280.1989;
RA Carsiotis M., Stocker B.A., Weinstein D.L., O'Brien A.D.;
RT "A Salmonella typhimurium virulence gene linked to flg.";
RL Infect. Immun. 57:3276-3280(1989).
RN [5]
RP SHOWS THAT FLGM IS THE REAL MOUSE VIRULENCE GENE IN THIS REGION.
RC STRAIN=SL3201;
RX PubMed=8288531; DOI=10.1128/jb.176.2.368-377.1994;
RA Schmitt C.K., Darnell S.C., Tesh V.L., Stocker B.A.D., O'Brien A.D.;
RT "Mutation of flgM attenuates virulence of Salmonella typhimurium, and
RT mutation of fliA represses the attenuated phenotype.";
RL J. Bacteriol. 176:368-377(1994).
CC -!- FUNCTION: Involved in peptidoglycan biosynthesis. Transports lipid-
CC linked peptidoglycan precursors from the inner to the outer leaflet of
CC the cytoplasmic membrane. {ECO:0000255|HAMAP-Rule:MF_02078}.
CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_02078}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_02078}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_02078}.
CC -!- SIMILARITY: Belongs to the MurJ/MviN family. {ECO:0000255|HAMAP-
CC Rule:MF_02078}.
CC -!- CAUTION: Was originally (PubMed:2680969) thought to be involved in
CC mouse virulence. It was later shown that the mutation responsible for
CC the virulence phenotype maps to nearby genes (PubMed:8288531).
CC {ECO:0000305|PubMed:2680969, ECO:0000305|PubMed:8288531}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z26133; CAA81134.1; -; Genomic_DNA.
DR EMBL; D25292; BAA04980.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL20100.1; -; Genomic_DNA.
DR PIR; S40271; S40271.
DR RefSeq; NP_460141.1; NC_003197.2.
DR RefSeq; WP_001155214.1; NC_003197.2.
DR AlphaFoldDB; P37169; -.
DR SMR; P37169; -.
DR STRING; 99287.STM1170; -.
DR TCDB; 2.A.66.4.1; the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.
DR PaxDb; P37169; -.
DR PRIDE; P37169; -.
DR EnsemblBacteria; AAL20100; AAL20100; STM1170.
DR GeneID; 1252688; -.
DR KEGG; stm:STM1170; -.
DR PATRIC; fig|99287.12.peg.1238; -.
DR HOGENOM; CLU_006797_5_3_6; -.
DR OMA; IFFVAFK; -.
DR PhylomeDB; P37169; -.
DR BioCyc; SENT99287:STM1170-MON; -.
DR UniPathway; UPA00219; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015648; F:lipid-linked peptidoglycan transporter activity; IBA:GO_Central.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0034204; P:lipid translocation; IBA:GO_Central.
DR GO; GO:0015836; P:lipid-linked peptidoglycan transport; IBA:GO_Central.
DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IBA:GO_Central.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR CDD; cd13123; MATE_MurJ_like; 1.
DR HAMAP; MF_02078; MurJ_MviN; 1.
DR InterPro; IPR004268; MurJ.
DR Pfam; PF03023; MurJ; 1.
DR PIRSF; PIRSF002869; MviN; 1.
DR PRINTS; PR01806; VIRFACTRMVIN.
DR TIGRFAMs; TIGR01695; murJ_mviN; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Cell shape;
KW Cell wall biogenesis/degradation; Membrane; Peptidoglycan synthesis;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..524
FT /note="Probable lipid II flippase MurJ"
FT /id="PRO_0000182014"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 146..166
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 250..270
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 284..304
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 322..342
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 367..387
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 396..416
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 420..440
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 456..476
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
FT TRANSMEM 494..514
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02078"
SQ SEQUENCE 524 AA; 57315 MW; 105BB31AF805E048 CRC64;
MQEFYARVWN TKEMNLLKSL AAVSSMTMFS RVLGFARDAI VARIFGAGMA TDAFFVAFKL
PNLLRRIFAE GAFSQAFVPI LAEYKSKQGE EATRIFVAYV SGLLTLALAV VTVAGMLAAP
WVIMVTAPGF ADTADKFALT TQLLRITFPY ILLISLASLV GAILNTWNRF SIPAFAPTFL
NISMIGFALF AAPYFNPPVL ALAWAVTVGG VLQLVYQLPY LKKIGMLVLP RINFRDTGAM
RVVKQMGPAI LGVSVSQISL IINTIFASFL ASGSVSWMYY ADRLMEFPSG VLGVALGTIL
LPSLSKSFAS GNHDEYCRLM DWGLRLCFLL ALPSAVALGI LAKPLTVSLF QYGKFTAFDA
AMTQRALIAY SVGLIGLIVV KVLAPGFYSR QDIKTPVKIA IVTLIMTQLM NLAFIGPLKH
AGLSLSIGLA ACLNASLLYW QLRKQNIFTP QPGWMWFLMR LIISVLVMAA VLFGVLHIMP
EWSQGSMLWR LLRLMAVVIA GIAAYFAALA VLGFKVKEFV RRTA
